BRENDA - Enzyme Database

Porphyrin biosynthesis in soybean callus tissue system. VIII. Isolation, purification and general properties of succinyl CoA synthetase

Wider, E.A.; Tigier, H.A.; Enzymologia 41, 217-231 (1971)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.2.1.5
8-hydroxyquinoline
5 mM, enhances activity
Glycine max
6.2.1.5
glutathione
activates
Glycine max
6.2.1.5
NaCN
10 mM , enhances activity
Glycine max
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.5
5,5'-dithiobis(2-nitrobenzoate)
-
Glycine max
6.2.1.5
acetate
-
Glycine max
6.2.1.5
ADP
-
Glycine max
6.2.1.5
AMP
-
Glycine max
6.2.1.5
ATP
above 10 mM
Glycine max
6.2.1.5
Butyrate
-
Glycine max
6.2.1.5
Ca2+
10 mM, 75% inhibition
Glycine max
6.2.1.5
Co2+
up to 1 mM complete inhibition
Glycine max
6.2.1.5
Cu2+
up to 10 mM complete inhibition
Glycine max
6.2.1.5
cystine
-
Glycine max
6.2.1.5
EDTA
-
Glycine max
6.2.1.5
GSSG
-
Glycine max
6.2.1.5
hemin
-
Glycine max
6.2.1.5
Hg2+
up to 1 mM complete inhibition
Glycine max
6.2.1.5
iodoacetamide
-
Glycine max
6.2.1.5
Iodosobenzoate
-
Glycine max
6.2.1.5
malonate
-
Glycine max
6.2.1.5
NEM
-
Glycine max
6.2.1.5
Pb2+
up to 10 mM complete inhibibition
Glycine max
6.2.1.5
PCMB
-
Glycine max
6.2.1.5
propionate
-
Glycine max
6.2.1.5
protoporphyrin IX
-
Glycine max
6.2.1.5
sodium arsenite
-
Glycine max
6.2.1.5
succinate
above 0.2 M
Glycine max
6.2.1.5
Zn2+
up to 1 mM complete inhibition
Glycine max
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.5
1.6
-
ATP
soluble enzyme
Glycine max
6.2.1.5
14
-
succinate
soluble enzyme
Glycine max
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.5
Mg2+
absolute requirement for either Mg2+ or Mn2+, optimal concentration of Mg2+ is 5 mM; Km: 1.5 mM, soluble enzyme
Glycine max
6.2.1.5
Mn2+
absolute requirement for either Mg2+ or Mn2+, Km: 3.5 mM
Glycine max
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.2.1.5
160000
-
gel filtration
Glycine max
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.5
additional information
Glycine max
enzyme is involved in the initial step of porphyrin biosynthesis
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.2.1.5
Glycine max
-
soybean
-
Purification (Commentary)
EC Number
Commentary
Organism
6.2.1.5
-
Glycine max
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.2.1.5
callus
-
Glycine max
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.2.1.5
additional information
-
-
Glycine max
Storage Stability
EC Number
Storage Stability
Organism
6.2.1.5
stable for several months in the frozen state
Glycine max
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.5
ATP + succinate + CoA
specific for ATP
805
Glycine max
ADP + phosphate + succinyl-CoA
-
-
-
-
6.2.1.5
ATP + succinate + CoA
specific for succinate
805
Glycine max
ADP + phosphate + succinyl-CoA
-
-
-
-
6.2.1.5
additional information
enzyme is involved in the initial step of porphyrin biosynthesis
805
Glycine max
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.2.1.5
38
-
-
Glycine max
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.5
7.9
8.3
-
Glycine max
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.2.1.5
7.1
9
pH 7.1: about 75% of maximal activity, pH 9.0: about 70% of maximal activity
Glycine max
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.2.1.5
8-hydroxyquinoline
5 mM, enhances activity
Glycine max
6.2.1.5
glutathione
activates
Glycine max
6.2.1.5
NaCN
10 mM , enhances activity
Glycine max
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.5
5,5'-dithiobis(2-nitrobenzoate)
-
Glycine max
6.2.1.5
acetate
-
Glycine max
6.2.1.5
ADP
-
Glycine max
6.2.1.5
AMP
-
Glycine max
6.2.1.5
ATP
above 10 mM
Glycine max
6.2.1.5
Butyrate
-
Glycine max
6.2.1.5
Ca2+
10 mM, 75% inhibition
Glycine max
6.2.1.5
Co2+
up to 1 mM complete inhibition
Glycine max
6.2.1.5
Cu2+
up to 10 mM complete inhibition
Glycine max
6.2.1.5
cystine
-
Glycine max
6.2.1.5
EDTA
-
Glycine max
6.2.1.5
GSSG
-
Glycine max
6.2.1.5
hemin
-
Glycine max
6.2.1.5
Hg2+
up to 1 mM complete inhibition
Glycine max
6.2.1.5
iodoacetamide
-
Glycine max
6.2.1.5
Iodosobenzoate
-
Glycine max
6.2.1.5
malonate
-
Glycine max
6.2.1.5
NEM
-
Glycine max
6.2.1.5
Pb2+
up to 10 mM complete inhibibition
Glycine max
6.2.1.5
PCMB
-
Glycine max
6.2.1.5
propionate
-
Glycine max
6.2.1.5
protoporphyrin IX
-
Glycine max
6.2.1.5
sodium arsenite
-
Glycine max
6.2.1.5
succinate
above 0.2 M
Glycine max
6.2.1.5
Zn2+
up to 1 mM complete inhibition
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.5
1.6
-
ATP
soluble enzyme
Glycine max
6.2.1.5
14
-
succinate
soluble enzyme
Glycine max
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.5
Mg2+
absolute requirement for either Mg2+ or Mn2+, optimal concentration of Mg2+ is 5 mM; Km: 1.5 mM, soluble enzyme
Glycine max
6.2.1.5
Mn2+
absolute requirement for either Mg2+ or Mn2+, Km: 3.5 mM
Glycine max
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.2.1.5
160000
-
gel filtration
Glycine max
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.5
additional information
Glycine max
enzyme is involved in the initial step of porphyrin biosynthesis
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.5
-
Glycine max
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.2.1.5
callus
-
Glycine max
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.2.1.5
additional information
-
-
Glycine max
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.2.1.5
stable for several months in the frozen state
Glycine max
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.5
ATP + succinate + CoA
specific for ATP
805
Glycine max
ADP + phosphate + succinyl-CoA
-
-
-
-
6.2.1.5
ATP + succinate + CoA
specific for succinate
805
Glycine max
ADP + phosphate + succinyl-CoA
-
-
-
-
6.2.1.5
additional information
enzyme is involved in the initial step of porphyrin biosynthesis
805
Glycine max
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.2.1.5
38
-
-
Glycine max
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.5
7.9
8.3
-
Glycine max
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.2.1.5
7.1
9
pH 7.1: about 75% of maximal activity, pH 9.0: about 70% of maximal activity
Glycine max