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Literature summary extracted from

  • Wider, E.A.; Tigier, H.A.
    Porphyrin biosynthesis in soybean callus tissue system. VIII. Isolation, purification and general properties of succinyl CoA synthetase (1971), Enzymologia, 41, 217-231.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.2.1.5 8-hydroxyquinoline 5 mM, enhances activity Glycine max
6.2.1.5 glutathione activates Glycine max
6.2.1.5 NaCN 10 mM , enhances activity Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.5 5,5'-dithiobis(2-nitrobenzoate)
-
 Glycine max
6.2.1.5 acetate
-
 Glycine max
6.2.1.5 ADP
-
 Glycine max
6.2.1.5 AMP
-
 Glycine max
6.2.1.5 ATP above 10 mM Glycine max
6.2.1.5 Butyrate
-
 Glycine max
6.2.1.5 Ca2+ 10 mM, 75% inhibition Glycine max
6.2.1.5 Co2+ up to 1 mM complete inhibition Glycine max
6.2.1.5 Cu2+ up to 10 mM complete inhibition Glycine max
6.2.1.5 cystine
-
 Glycine max
6.2.1.5 EDTA
-
 Glycine max
6.2.1.5 GSSG
-
 Glycine max
6.2.1.5 hemin
-
 Glycine max
6.2.1.5 Hg2+ up to 1 mM complete inhibition Glycine max
6.2.1.5 iodoacetamide
-
 Glycine max
6.2.1.5 Iodosobenzoate
-
 Glycine max
6.2.1.5 malonate
-
 Glycine max
6.2.1.5 NEM
-
 Glycine max
6.2.1.5 Pb2+ up to 10 mM complete inhibibition Glycine max
6.2.1.5 PCMB
-
 Glycine max
6.2.1.5 propionate
-
 Glycine max
6.2.1.5 protoporphyrin IX
-
 Glycine max
6.2.1.5 sodium arsenite
-
 Glycine max
6.2.1.5 succinate above 0.2 M Glycine max
6.2.1.5 Zn2+ up to 1 mM complete inhibition Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.5 1.6
-
 ATP soluble enzyme Glycine max
6.2.1.5 14
-
 succinate soluble enzyme Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.5 Mg2+ Km: 1.5 mM, soluble enzyme Glycine max
6.2.1.5 Mg2+ absolute requirement for either Mg2+ or Mn2+, optimal concentration of Mg2+ is 5 mM Glycine max
6.2.1.5 Mn2+ absolute requirement for either Mg2+ or Mn2+, Km: 3.5 mM Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.5 160000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.5 additional information Glycine max enzyme is involved in the initial step of porphyrin biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.5 Glycine max
-
 soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.5
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.5 callus
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.5 additional information
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
6.2.1.5 stable for several months in the frozen state Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.5 ATP + succinate + CoA specific for ATP Glycine max ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA specific for succinate Glycine max ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 additional information enzyme is involved in the initial step of porphyrin biosynthesis Glycine max ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.5 38
-
-
 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.5 7.9 8.3
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.5 7.1 9 pH 7.1: about 75% of maximal activity, pH 9.0: about 70% of maximal activity Glycine max