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Literature summary extracted from

  • Danson, M.J.; Black, S.C.; Woodland, D.L.; Wood, P.A.
    Citric acid cycle enzymes of the archaebacteria: citrate synthase and succinate thiokinase (1985), FEBS Lett., 179, 120-124.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 2-oxoglutarate
-
 Haloarcula vallismortis
2.3.3.16 2-oxoglutarate no inhibition Halobacterium salinarum
2.3.3.16 2-oxoglutarate
-
 Haloferax volcanii
2.3.3.16 2-oxoglutarate
-
 Methanosarcina barkeri
2.3.3.16 2-oxoglutarate
-
 Natronobacterium gregoryi
2.3.3.16 2-oxoglutarate
-
 Natronococcus occultus
2.3.3.16 2-oxoglutarate no inhibition Natronomonas pharaonis
2.3.3.16 2-oxoglutarate
-
 Sulfolobus acidocaldarius
2.3.3.16 2-oxoglutarate
-
 Thermoplasma acidophilum
2.3.3.16 ATP
-
 Haloarcula vallismortis
2.3.3.16 ATP
-
 Halobacterium salinarum
2.3.3.16 ATP
-
 Haloferax volcanii
2.3.3.16 ATP
-
 Methanosarcina barkeri
2.3.3.16 ATP
-
 Natronobacterium gregoryi
2.3.3.16 ATP
-
 Natronococcus occultus
2.3.3.16 ATP
-
 Natronomonas pharaonis
2.3.3.16 ATP
-
 Sulfolobus acidocaldarius
2.3.3.16 ATP
-
 Thermoplasma acidophilum
2.3.3.16 NADH
-
 Halobacterium salinarum
2.3.3.16 NADH
-
 Haloferax volcanii
2.3.3.16 NADH
-
 Methanosarcina barkeri
2.3.3.16 NADH
-
 Natronobacterium gregoryi
2.3.3.16 NADH
-
 Natronomonas pharaonis
2.3.3.16 NADH
-
 Sulfolobus acidocaldarius
2.3.3.16 NADH
-
 Thermoplasma acidophilum
6.2.1.5 2-oxoglutarate
-
 Escherichia coli
6.2.1.5 2-oxoglutarate no inhibition Halobacterium salinarum
6.2.1.5 2-oxoglutarate
-
 Haloferax volcanii
6.2.1.5 2-oxoglutarate
-
 Natronobacterium gregoryi
6.2.1.5 2-oxoglutarate no inhibition Natronomonas pharaonis
6.2.1.5 2-oxoglutarate
-
 Priestia megaterium
6.2.1.5 2-oxoglutarate
-
 Sulfolobus acidocaldarius
6.2.1.5 2-oxoglutarate no inhibition Sus scrofa
6.2.1.5 2-oxoglutarate
-
 Thermoplasma acidophilum
6.2.1.5 ATP
-
 Escherichia coli
6.2.1.5 ATP
-
 Halobacterium salinarum
6.2.1.5 ATP
-
 Haloferax volcanii
6.2.1.5 ATP
-
 Natronobacterium gregoryi
6.2.1.5 ATP
-
 Natronomonas pharaonis
6.2.1.5 ATP
-
 Priestia megaterium
6.2.1.5 ATP
-
 Sulfolobus acidocaldarius
6.2.1.5 ATP
-
 Sus scrofa
6.2.1.5 ATP
-
 Thermoplasma acidophilum
6.2.1.5 NADH
-
 Escherichia coli
6.2.1.5 NADH
-
 Halobacterium salinarum
6.2.1.5 NADH
-
 Haloferax volcanii
6.2.1.5 NADH
-
 Natronobacterium gregoryi
6.2.1.5 NADH
-
 Natronomonas pharaonis
6.2.1.5 NADH
-
 Priestia megaterium
6.2.1.5 NADH
-
 Sulfolobus acidocaldarius
6.2.1.5 NADH
-
 Sus scrofa
6.2.1.5 NADH
-
 Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.005
-
 oxaloacetate
-
 Thermoplasma acidophilum
2.3.3.16 0.006
-
 acetyl-CoA
-
 Thermoplasma acidophilum
2.3.3.16 0.007
-
 acetyl-CoA
-
 Methanosarcina barkeri
2.3.3.16 0.01
-
 acetyl-CoA
-
 Sulfolobus acidocaldarius
2.3.3.16 0.018
-
 oxaloacetate
-
 Methanosarcina barkeri
2.3.3.16 0.02
-
 oxaloacetate
-
 Sulfolobus acidocaldarius
2.3.3.16 0.038
-
 oxaloacetate
-
 Haloferax volcanii
2.3.3.16 0.058
-
 oxaloacetate
-
 Halobacterium salinarum
2.3.3.16 0.063
-
 oxaloacetate
-
 Natronobacterium gregoryi
2.3.3.16 0.075
-
 acetyl-CoA
-
 Haloferax volcanii
2.3.3.16 0.11
-
 acetyl-CoA
-
 Natronobacterium gregoryi
2.3.3.16 0.21
-
 oxaloacetate
-
 Natronomonas pharaonis
2.3.3.16 0.211
-
 acetyl-CoA
-
 Halobacterium salinarum
2.3.3.16 0.45
-
 acetyl-CoA
-
 Natronomonas pharaonis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 83000
-
 gel filtration Sulfolobus acidocaldarius
2.3.3.16 85000
-
 gel filtration Thermoplasma acidophilum
2.3.3.16 105000
-
 gel filtration Haloferax volcanii
2.3.3.16 105000
-
 gel filtration Natronococcus occultus
2.3.3.16 106000
-
 gel filtration Natronomonas pharaonis
2.3.3.16 111000
-
 gel filtration Halobacterium salinarum
2.3.3.16 112000
-
 gel filtration Haloarcula vallismortis
2.3.3.16 112000
-
 gel filtration Natronobacterium gregoryi
6.2.1.5 68000
-
 gel filtration Thermoplasma acidophilum
6.2.1.5 75000
-
 gel filtration Sus scrofa
6.2.1.5 75000
-
 gel filtration Priestia megaterium
6.2.1.5 136000
-
 gel filtration Escherichia coli
6.2.1.5 170000
-
 gel filtration Haloferax volcanii
6.2.1.5 171000
-
 gel filtration Natronobacterium gregoryi
6.2.1.5 174000
-
 gel filtration Haloarcula vallismortis
6.2.1.5 174000
-
 gel filtration Natronomonas pharaonis
6.2.1.5 184000
-
 gel filtration Natronococcus occultus

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Haloarcula vallismortis
-
-
-
2.3.3.16 Halobacterium salinarum
-
-
-
2.3.3.16 Haloferax volcanii
-
-
-
2.3.3.16 Methanosarcina barkeri
-
-
-
2.3.3.16 Natronobacterium gregoryi
-
-
-
2.3.3.16 Natronococcus occultus
-
-
-
2.3.3.16 Natronomonas pharaonis
-
-
-
2.3.3.16 Sulfolobus acidocaldarius
-
-
-
2.3.3.16 Thermoplasma acidophilum
-
-
-
6.2.1.5 Escherichia coli
-
-
-
6.2.1.5 Haloarcula vallismortis
-
-
-
6.2.1.5 Halobacterium salinarum
-
-
-
6.2.1.5 Haloferax volcanii
-
-
-
6.2.1.5 Natronobacterium gregoryi
-
-
-
6.2.1.5 Natronococcus occultus
-
-
-
6.2.1.5 Natronomonas pharaonis
-
-
-
6.2.1.5 Priestia megaterium
-
-
-
6.2.1.5 Sulfolobus acidocaldarius
-
 strain DSM639
-
6.2.1.5 Sulfolobus acidocaldarius DSM 639
-
 strain DSM639
-
6.2.1.5 Sus scrofa
-
-
-
6.2.1.5 Thermoplasma acidophilum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.5 heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 0.002
-
-
 Methanosarcina barkeri
2.3.3.16 0.014
-
-
 Natronobacterium gregoryi
2.3.3.16 0.026
-
-
 Natronomonas pharaonis
2.3.3.16 0.051
-
-
 Halobacterium salinarum
2.3.3.16 0.061
-
-
 Haloferax volcanii
2.3.3.16 0.172
-
-
 Thermoplasma acidophilum
2.3.3.16 0.28
-
-
 Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Methanosarcina barkeri citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Halobacterium salinarum citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Haloarcula vallismortis citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Haloferax volcanii citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Natronomonas pharaonis citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Natronobacterium gregoryi citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Natronococcus occultus citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Sulfolobus acidocaldarius citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Thermoplasma acidophilum citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Sulfolobus acidocaldarius DSM 639 citrate + CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Escherichia coli ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Sus scrofa ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Priestia megaterium ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Halobacterium salinarum ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Haloarcula vallismortis ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Haloferax volcanii ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Natronomonas pharaonis ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Natronobacterium gregoryi ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Natronococcus occultus ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Sulfolobus acidocaldarius ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Thermoplasma acidophilum ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA no activity with GTP/GDP Sulfolobus acidocaldarius DSM 639 ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Escherichia coli GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Sus scrofa GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Priestia megaterium GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Halobacterium salinarum GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Haloarcula vallismortis GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Haloferax volcanii GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Natronomonas pharaonis GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Natronobacterium gregoryi GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Natronococcus occultus GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Sulfolobus acidocaldarius GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Thermoplasma acidophilum GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 GTP + succinate + CoA no activity with GDP/GTP Sulfolobus acidocaldarius DSM 639 GDP + phosphate + succinyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.5 dimer
-
 Sus scrofa
6.2.1.5 dimer
-
 Priestia megaterium
6.2.1.5 tetramer
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 30
-
-
 Natronomonas pharaonis
2.3.3.16 30
-
 enzyme assay at Methanosarcina barkeri
2.3.3.16 30
-
 enzyme assay at Halobacterium salinarum
2.3.3.16 30
-
 enzyme assay at Haloferax volcanii
2.3.3.16 30
-
 enzyme assay at Natronobacterium gregoryi
2.3.3.16 55
-
 enzyme assay at Sulfolobus acidocaldarius
2.3.3.16 55
-
 enzyme assay at Thermoplasma acidophilum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 0.1
-
 NADH below Methanosarcina barkeri
2.3.3.16 0.3
-
 ATP below Methanosarcina barkeri
2.3.3.16 0.3
-
 2-oxoglutarate below Methanosarcina barkeri
2.3.3.16 0.9
-
 ATP
-
 Sulfolobus acidocaldarius
2.3.3.16 1.9
-
 ATP
-
 Haloferax volcanii
2.3.3.16 2.2
-
 ATP
-
 Thermoplasma acidophilum
2.3.3.16 2.2
-
 2-oxoglutarate
-
 Sulfolobus acidocaldarius
2.3.3.16 3.2
-
 NADH
-
 Natronobacterium gregoryi
2.3.3.16 4.2
-
 ATP
-
 Natronobacterium gregoryi
2.3.3.16 4.6
-
 NADH
-
 Sulfolobus acidocaldarius
2.3.3.16 5.2
-
 NADH
-
 Natronomonas pharaonis
2.3.3.16 5.4
-
 NADH
-
 Thermoplasma acidophilum
2.3.3.16 5.4
-
 2-oxoglutarate
-
 Thermoplasma acidophilum
2.3.3.16 5.6
-
 ATP
-
 Halobacterium salinarum
2.3.3.16 10
-
 NADH above Halobacterium salinarum
2.3.3.16 10
-
 NADH above Haloferax volcanii
2.3.3.16 20
-
 2-oxoglutarate above Haloferax volcanii
2.3.3.16 20
-
 2-oxoglutarate above Natronobacterium gregoryi
2.3.3.16 30
-
 ATP above Natronomonas pharaonis