BRENDA - Enzyme Database

Isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS-3

Chang, K.H.; Liang, P.H.; Beck, W.; Scholten, J.D.; Dunaway-Mariano, D.; Biochemistry 31, 5605-5610 (1992)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.23
expression in Escherichia coli
Pseudomonas sp.
3.8.1.7
expression in Escherichia coli
Pseudomonas sp.
6.2.1.33
expression in Escherichia coli
Pseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.23
0.0045
-
4-hydroxybenzoyl-CoA
-
Pseudomonas sp.
3.1.2.23
0.19
-
4-chlorobenzoyl-CoA
-
Pseudomonas sp.
3.1.2.23
0.2
-
benzoyl-CoA
-
Pseudomonas sp.
3.8.1.7
0.015
-
4-chlorobenzoyl-CoA
-
Pseudomonas sp.
6.2.1.33
0.009
-
4-Chlorobenzoate
-
Pseudomonas sp.
6.2.1.33
0.08
-
CoA
-
Pseudomonas sp.
6.2.1.33
0.1
-
MgATP2-
-
Pseudomonas sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.33
Co2+
activates
Pseudomonas sp.
6.2.1.33
Mg2+
required
Pseudomonas sp.
6.2.1.33
Mn2+
activates
Pseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.23
16000
-
4 * 16000, SDS-PAGE
Pseudomonas sp.
3.1.2.23
16107
-
4 * 16107, calculation from nucleotide sequence
Pseudomonas sp.
3.1.2.23
66000
-
gel filtration
Pseudomonas sp.
3.8.1.7
29847
-
4 * 29847, calculation from nucleotide sequence
Pseudomonas sp.
3.8.1.7
31000
-
4 * 31000, SDS-PAGE
Pseudomonas sp.
3.8.1.7
122000
-
gel filtration
Pseudomonas sp.
6.2.1.33
57000
-
2 * 57000, SDS-PAGE
Pseudomonas sp.
6.2.1.33
114000
-
gel filtration
Pseudomonas sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.23
Pseudomonas sp.
-
-
-
3.1.2.23
Pseudomonas sp. CBS-3
-
-
-
3.8.1.7
Pseudomonas sp.
-
-
-
3.8.1.7
Pseudomonas sp. CBS-3
-
-
-
6.2.1.33
Pseudomonas sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.23
-
Pseudomonas sp.
3.8.1.7
-
Pseudomonas sp.
6.2.1.33
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.23
additional information
-
-
Pseudomonas sp.
3.8.1.7
additional information
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.23
4-Chlorobenzoyl-CoA + H2O
-
780
Pseudomonas sp.
4-Chlorobenzoate + CoA
-
780
Pseudomonas sp.
-
3.1.2.23
4-Chlorobenzoyl-CoA + H2O
-
780
Pseudomonas sp. CBS-3
4-Chlorobenzoate + CoA
-
780
Pseudomonas sp. CBS-3
-
3.1.2.23
4-Hydroxybenzoyl-CoA + H2O
-
780
Pseudomonas sp.
4-Hydroxybenzoate + CoA
-
780
Pseudomonas sp.
-
3.1.2.23
4-Hydroxybenzoyl-CoA + H2O
-
780
Pseudomonas sp. CBS-3
4-Hydroxybenzoate + CoA
-
780
Pseudomonas sp. CBS-3
-
3.1.2.23
Benzoyl-CoA + H2O
-
780
Pseudomonas sp.
Benzoate + CoA
-
780
Pseudomonas sp.
-
3.1.2.23
Benzoyl-CoA + H2O
-
780
Pseudomonas sp. CBS-3
Benzoate + CoA
-
780
Pseudomonas sp. CBS-3
-
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
780
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
-
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
780
Pseudomonas sp. CBS-3
4-hydroxybenzoyl-CoA + chloride
-
-
-
-
6.2.1.33
4-Bromobenzoate + CoA + ATP
-
780
Pseudomonas sp.
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
4-Chlorobenzoate + CoA + ATP
-
780
Pseudomonas sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
4-Chlorobenzoate + CoA + ATP
-
780
Pseudomonas sp. CBS-3
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
4-Iodobenzoate + CoA + ATP
-
780
Pseudomonas sp.
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
4-Methylbenzoate + CoA + ATP
-
780
Pseudomonas sp.
4-Methylbenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
Benzoate + CoA + ATP
-
780
Pseudomonas sp.
Benzoyl-CoA + AMP + diphosphate
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.23
tetramer
4 * 16000, SDS-PAGE; 4 * 16107, calculation from nucleotide sequence
Pseudomonas sp.
3.8.1.7
tetramer
4 * 29847, calculation from nucleotide sequence; 4 * 31000, SDS-PAGE
Pseudomonas sp.
6.2.1.33
dimer
2 * 57000, SDS-PAGE
Pseudomonas sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.23
0.123
-
4-Chlorobenzoate
-
Pseudomonas sp.
3.1.2.23
0.26
-
benzoyl-CoA
-
Pseudomonas sp.
3.1.2.23
6.8
-
4-hydroxybenzoyl-CoA
-
Pseudomonas sp.
3.8.1.7
0.3
-
4-chlorobenzoyl-CoA
-
Pseudomonas sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.23
expression in Escherichia coli
Pseudomonas sp.
3.8.1.7
expression in Escherichia coli
Pseudomonas sp.
6.2.1.33
expression in Escherichia coli
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.23
0.0045
-
4-hydroxybenzoyl-CoA
-
Pseudomonas sp.
3.1.2.23
0.19
-
4-chlorobenzoyl-CoA
-
Pseudomonas sp.
3.1.2.23
0.2
-
benzoyl-CoA
-
Pseudomonas sp.
3.8.1.7
0.015
-
4-chlorobenzoyl-CoA
-
Pseudomonas sp.
6.2.1.33
0.009
-
4-Chlorobenzoate
-
Pseudomonas sp.
6.2.1.33
0.08
-
CoA
-
Pseudomonas sp.
6.2.1.33
0.1
-
MgATP2-
-
Pseudomonas sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.33
Co2+
activates
Pseudomonas sp.
6.2.1.33
Mg2+
required
Pseudomonas sp.
6.2.1.33
Mn2+
activates
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.23
16000
-
4 * 16000, SDS-PAGE
Pseudomonas sp.
3.1.2.23
16107
-
4 * 16107, calculation from nucleotide sequence
Pseudomonas sp.
3.1.2.23
66000
-
gel filtration
Pseudomonas sp.
3.8.1.7
29847
-
4 * 29847, calculation from nucleotide sequence
Pseudomonas sp.
3.8.1.7
31000
-
4 * 31000, SDS-PAGE
Pseudomonas sp.
3.8.1.7
122000
-
gel filtration
Pseudomonas sp.
6.2.1.33
57000
-
2 * 57000, SDS-PAGE
Pseudomonas sp.
6.2.1.33
114000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.23
-
Pseudomonas sp.
3.8.1.7
-
Pseudomonas sp.
6.2.1.33
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.23
additional information
-
-
Pseudomonas sp.
3.8.1.7
additional information
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.23
4-Chlorobenzoyl-CoA + H2O
-
780
Pseudomonas sp.
4-Chlorobenzoate + CoA
-
780
Pseudomonas sp.
-
3.1.2.23
4-Chlorobenzoyl-CoA + H2O
-
780
Pseudomonas sp. CBS-3
4-Chlorobenzoate + CoA
-
780
Pseudomonas sp. CBS-3
-
3.1.2.23
4-Hydroxybenzoyl-CoA + H2O
-
780
Pseudomonas sp.
4-Hydroxybenzoate + CoA
-
780
Pseudomonas sp.
-
3.1.2.23
4-Hydroxybenzoyl-CoA + H2O
-
780
Pseudomonas sp. CBS-3
4-Hydroxybenzoate + CoA
-
780
Pseudomonas sp. CBS-3
-
3.1.2.23
Benzoyl-CoA + H2O
-
780
Pseudomonas sp.
Benzoate + CoA
-
780
Pseudomonas sp.
-
3.1.2.23
Benzoyl-CoA + H2O
-
780
Pseudomonas sp. CBS-3
Benzoate + CoA
-
780
Pseudomonas sp. CBS-3
-
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
780
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
-
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
780
Pseudomonas sp. CBS-3
4-hydroxybenzoyl-CoA + chloride
-
-
-
-
6.2.1.33
4-Bromobenzoate + CoA + ATP
-
780
Pseudomonas sp.
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
4-Chlorobenzoate + CoA + ATP
-
780
Pseudomonas sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
4-Chlorobenzoate + CoA + ATP
-
780
Pseudomonas sp. CBS-3
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
4-Iodobenzoate + CoA + ATP
-
780
Pseudomonas sp.
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
4-Methylbenzoate + CoA + ATP
-
780
Pseudomonas sp.
4-Methylbenzoyl-CoA + AMP + diphosphate
-
-
-
-
6.2.1.33
Benzoate + CoA + ATP
-
780
Pseudomonas sp.
Benzoyl-CoA + AMP + diphosphate
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.23
tetramer
4 * 16000, SDS-PAGE; 4 * 16107, calculation from nucleotide sequence
Pseudomonas sp.
3.8.1.7
tetramer
4 * 29847, calculation from nucleotide sequence; 4 * 31000, SDS-PAGE
Pseudomonas sp.
6.2.1.33
dimer
2 * 57000, SDS-PAGE
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.23
0.123
-
4-Chlorobenzoate
-
Pseudomonas sp.
3.1.2.23
0.26
-
benzoyl-CoA
-
Pseudomonas sp.
3.1.2.23
6.8
-
4-hydroxybenzoyl-CoA
-
Pseudomonas sp.
3.8.1.7
0.3
-
4-chlorobenzoyl-CoA
-
Pseudomonas sp.