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Literature summary extracted from

  • Chang, K.H.; Liang, P.H.; Beck, W.; Scholten, J.D.; Dunaway-Mariano, D.
    Isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS-3 (1992), Biochemistry, 31, 5605-5610.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.23 expression in Escherichia coli Pseudomonas sp.
3.8.1.7 expression in Escherichia coli Pseudomonas sp.
6.2.1.33 expression in Escherichia coli Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.23 0.0045
-
 4-hydroxybenzoyl-CoA
-
 Pseudomonas sp.
3.1.2.23 0.19
-
 4-chlorobenzoyl-CoA
-
 Pseudomonas sp.
3.1.2.23 0.2
-
 benzoyl-CoA
-
 Pseudomonas sp.
3.8.1.7 0.015
-
 4-chlorobenzoyl-CoA
-
 Pseudomonas sp.
6.2.1.33 0.009
-
 4-Chlorobenzoate
-
 Pseudomonas sp.
6.2.1.33 0.08
-
 CoA
-
 Pseudomonas sp.
6.2.1.33 0.1
-
 ATP
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.33 Co2+ activates Pseudomonas sp.
6.2.1.33 Mg2+ required Pseudomonas sp.
6.2.1.33 Mn2+ activates Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.23 16000
-
 4 * 16000, SDS-PAGE Pseudomonas sp.
3.1.2.23 16107
-
 4 * 16107, calculation from nucleotide sequence Pseudomonas sp.
3.1.2.23 66000
-
 gel filtration Pseudomonas sp.
3.8.1.7 29847
-
 4 * 29847, calculation from nucleotide sequence Pseudomonas sp.
3.8.1.7 31000
-
 4 * 31000, SDS-PAGE Pseudomonas sp.
3.8.1.7 122000
-
 gel filtration Pseudomonas sp.
6.2.1.33 57000
-
 2 * 57000, SDS-PAGE Pseudomonas sp.
6.2.1.33 114000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.23 Pseudomonas sp.
-
-
-
3.1.2.23 Pseudomonas sp. CBS-3
-
-
-
3.8.1.7 Pseudomonas sp.
-
-
-
3.8.1.7 Pseudomonas sp. CBS-3
-
-
-
6.2.1.33 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.23
-
 Pseudomonas sp.
3.8.1.7
-
 Pseudomonas sp.
6.2.1.33
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.23 additional information
-
-
 Pseudomonas sp.
3.8.1.7 additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.23 4-Chlorobenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-Chlorobenzoate + CoA
-
 ?
3.1.2.23 4-Chlorobenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 4-Chlorobenzoate + CoA
-
 ?
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.23 Benzoyl-CoA + H2O
-
 Pseudomonas sp. Benzoate + CoA
-
 ?
3.1.2.23 Benzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 Benzoate + CoA
-
 ?
3.8.1.7 4-chlorobenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-hydroxybenzoyl-CoA + chloride
-
 ?
3.8.1.7 4-chlorobenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 4-hydroxybenzoyl-CoA + chloride
-
 ?
6.2.1.33 4-Bromobenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Bromobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Chlorobenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Chlorobenzoate + CoA + ATP
-
 Pseudomonas sp. CBS-3 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Iodobenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Iodobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Methylbenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Methylbenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 ATP + 4-chlorobenzoate + CoA ATP in form of MgATP2- Pseudomonas sp. AMP + diphosphate + 4-chlorobenzoyl-CoA
-
 ?
6.2.1.33 Benzoate + CoA + ATP
-
 Pseudomonas sp. Benzoyl-CoA + AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.23 tetramer 4 * 16107, calculation from nucleotide sequence Pseudomonas sp.
3.1.2.23 tetramer 4 * 16000, SDS-PAGE Pseudomonas sp.
3.8.1.7 tetramer 4 * 29847, calculation from nucleotide sequence Pseudomonas sp.
3.8.1.7 tetramer 4 * 31000, SDS-PAGE Pseudomonas sp.
6.2.1.33 dimer 2 * 57000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.23 0.123
-
 4-Chlorobenzoate
-
 Pseudomonas sp.
3.1.2.23 0.26
-
 benzoyl-CoA
-
 Pseudomonas sp.
3.1.2.23 6.8
-
 4-hydroxybenzoyl-CoA
-
 Pseudomonas sp.
3.8.1.7 0.3
-
 4-chlorobenzoyl-CoA
-
 Pseudomonas sp.