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Literature summary extracted from

  • Loeffler, F.; Mueller, R.
    Characterization of 4-chlorobenzoyl-coenzyme A ligase from the 4-chlorobenzoate degrading strain Pseudomonas sp. CBS3 (1992), DECHEMA Biotechnol. Conf. (Pt. A. Microbial Principles in Bioprocesses: Cell Culture Technology - Downstream Processing and Recovery), 5, 25-28.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.33 Ag+
-
 Pseudomonas sp.
6.2.1.33 Cu2+
-
 Pseudomonas sp.
6.2.1.33 Fe2+
-
 Pseudomonas sp.
6.2.1.33 Fe3+
-
 Pseudomonas sp.
6.2.1.33 SDS
-
 Pseudomonas sp.
6.2.1.33 Zn2+
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.33 0.05 0.065 4-Chlorobenzoate
-
 Pseudomonas sp.
6.2.1.33 0.11 0.19 CoA
-
 Pseudomonas sp.
6.2.1.33 2.4 3.5 ATP
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.33 4-Chlorobenzoate + CoA + ATP Pseudomonas sp. induced by growth with 4-chlorobenzoate as sole carbon source ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.33 Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.33 4-Bromobenzoate + CoA + ATP at 105% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Bromobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Chlorobenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Chlorobenzoate + CoA + ATP induced by growth with 4-chlorobenzoate as sole carbon source Pseudomonas sp. ?
-
 ?
6.2.1.33 4-Fluorobenzoate + CoA + ATP at 36% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Fluorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Iodobenzoate + CoA + ATP at 90% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Iodobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Methoxybenzoate + CoA + ATP at 9% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Methoxybenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Methylbenzoate + CoA + ATP at 22% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Methylbenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Nitrobenzoate + CoA + ATP at 7% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Nitrobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 Benzoate + CoA + ATP at 5% of the activity with 4-chlorobenzoate Pseudomonas sp. Benzoyl-CoA + AMP + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.33 35
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.33 8.4
-
-
 Pseudomonas sp.