Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chang, K.H.; Xiang, H.; Dunaway-Mariano, D.
    Acyl-adenylate motif of the acyl-adenylate/thioester-forming enzyme superfamily: a site-directed mutagenesis study with the Pseudomonas sp. strain CBS3 4-chlorobenzoate:coenzyme A ligase (1997), Biochemistry, 36, 15650-15659.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.33 E306Q mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.
6.2.1.33 G163I mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.
6.2.1.33 G166I mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.
6.2.1.33 K169M mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.
6.2.1.33 P168A mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.33 0.014
-
 4-Chlorobenzoate wild-type Pseudomonas sp.
6.2.1.33 0.02
-
 4-Chlorobenzoate mutant K169M Pseudomonas sp.
6.2.1.33 0.062
-
 CoA mutant G163I Pseudomonas sp.
6.2.1.33 0.073
-
 4-Chlorobenzoate mutant G166I Pseudomonas sp.
6.2.1.33 0.076
-
 CoA mutant K169M Pseudomonas sp.
6.2.1.33 0.085
-
 CoA wild-type Pseudomonas sp.
6.2.1.33 0.105
-
 4-Chlorobenzoate mutant G163I Pseudomonas sp.
6.2.1.33 0.108
-
 CoA mutant G166I Pseudomonas sp.
6.2.1.33 0.168
-
 CoA mutant E306Q Pseudomonas sp.
6.2.1.33 0.253
-
 4-Chlorobenzoate mutant E306Q Pseudomonas sp.
6.2.1.33 0.308
-
 ATP wild-type Pseudomonas sp.
6.2.1.33 0.321
-
 ATP mutant E306Q Pseudomonas sp.
6.2.1.33 0.83
-
 ATP mutant K169M Pseudomonas sp.
6.2.1.33 0.981
-
 ATP mutant G166I Pseudomonas sp.
6.2.1.33 1.314
-
 ATP mutant G163I Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.33 Pseudomonas sp.
-
-
-
6.2.1.33 Pseudomonas sp.
-
 wild-type and mutant enzymes G163I, G166I, P168A, K169M and E306Q
-
6.2.1.33 Pseudomonas sp. CBS3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.33
-
 Pseudomonas sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
6.2.1.33 4-Chlorobenzoate + CoA + ATP = 4-chlorobenzoyl-CoA + AMP + diphosphate two-step reaction consisting of the adenylation of 4-chlorobenzoate with adenosine 5'-triphosphate followed by acyl transfer from 4-chlorobenzoyl adenosine 5'-monophosphate diester Pseudomonas sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.33 4-Chlorobenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Chlorobenzoate + CoA + ATP
-
 Pseudomonas sp. CBS3 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.33 0.036
-
 4-Chlorobenzoate mutant G163I Pseudomonas sp.
6.2.1.33 0.039
-
 ATP mutant G163I Pseudomonas sp.
6.2.1.33 0.04
-
 CoA mutant G163I Pseudomonas sp.
6.2.1.33 0.7
-
 4-Chlorobenzoate mutant E306Q Pseudomonas sp.
6.2.1.33 0.8
-
 ATP mutant E306Q Pseudomonas sp.
6.2.1.33 0.9
-
 ATP mutant E306Q Pseudomonas sp.
6.2.1.33 2.3
-
 4-Chlorobenzoate mutant G166I Pseudomonas sp.
6.2.1.33 2.7
-
 CoA mutant G166I Pseudomonas sp.
6.2.1.33 3.3
-
 ATP mutant G166I Pseudomonas sp.
6.2.1.33 9.4
-
 ATP mutant K169M Pseudomonas sp.
6.2.1.33 9.8
-
 4-Chlorobenzoate mutant K169M Pseudomonas sp.
6.2.1.33 16
-
 ATP mutant K169M Pseudomonas sp.
6.2.1.33 34
-
 4-Chlorobenzoate wild-type Pseudomonas sp.
6.2.1.33 39
-
 CoA wild-type Pseudomonas sp.
6.2.1.33 54
-
 ATP wild-type Pseudomonas sp.