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Literature summary extracted from

  • Xuan, G.; Xiao, Q.; Wang, J.; Lin, H.
    Cloning and expression of the flavin reductase LuxG from Photobacterium leiognathi YL and its improvement for NADH detection (2020), Photochem. Photobiol. Sci., 19, 274-280 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.1.42 analysis Photobacterium leiognathi Lux G might contribute to the development of a NADH detection kit Photobacterium leiognathi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.42 gene luxG, phylogenetic analysis, cloning and recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Photobacterium leiognathi

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.42 additional information establishment of a coupled pure enzyme bioluminescent system and usage for NADH detection Photobacterium leiognathi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.42 FMN + NADH + H+ Photobacterium leiognathi
-
 FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+ Photobacterium leiognathi YL
-
 FMNH2 + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.42 Photobacterium leiognathi P29237
-
-
1.5.1.42 Photobacterium leiognathi YL P29237
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.42 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Photobacterium leiognathi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.42 FMN + NADH + H+
-
 Photobacterium leiognathi FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+
-
 Photobacterium leiognathi YL FMNH2 + NAD+
-
 ?
1.5.1.42 additional information establishment of a coupled pure enzyme bioluminescent system and usage for quantitative detection, method evaluation Photobacterium leiognathi ?
-
-
1.5.1.42 additional information establishment of a coupled pure enzyme bioluminescent system and usage for quantitative detection, method evaluation Photobacterium leiognathi YL ?
-
-

Subunits

EC Number Subunits Comment Organism
1.5.1.42 ? x * 26000, recombinant His-tagged enzyme, SDS-PAGE Photobacterium leiognathi

Synonyms

EC Number Synonyms Comment Organism
1.5.1.42 flavin reductase
-
 Photobacterium leiognathi
1.5.1.42 LuxG
-
 Photobacterium leiognathi
1.5.1.42 NADH:flavin oxidoreductase
-
 Photobacterium leiognathi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.42 40
-
 recombinant His-tagged enzyme Photobacterium leiognathi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.42 8
-
 recombinant His-tagged enzyme Photobacterium leiognathi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.42 NADH
-
 Photobacterium leiognathi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.1.42 Photobacterium leiognathi recombinant His-tagged enzyme, isoelectric focusing
-
 6

General Information

EC Number General Information Comment Organism
1.5.1.42 evolution phylogenetic analysis demonstrats that Photobacterium leiognathi strain YL LuxG has a rather distant evolutionary relationship with Frase I of Aliivibrio fischeri and Frp of Vibrio harveyi, but a close evolutionary relationship with Fre from Escherichia coli, which are all enzymes related to oxidoreductases. Changes in the functionally conserved sites contribute to the functional divergence of LuxG and Fre. Bioinformatics analysis of LuxG sequences in bacteria, overview. Structure comparisons Photobacterium leiognathi
1.5.1.42 physiological function LuxG supplies reduced flavin mononucleotide (FMN) for bacterial luminescence by catalyzing the oxidation of nicotinamide adenine dinucleotide hydrogen (NADH) Photobacterium leiognathi