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Literature summary extracted from

  • Di Matteo, A.; Federici, L.; Masulli, M.; Carletti, E.; Santorelli, D.; Cassidy, J.; Paradisi, F.; Di Ilio, C.; Allocati, N.
    Structural characterization of the Xi class glutathione transferase from the haloalkaliphilic archaeon Natrialba magadii (2019), Front. Microbiol., 10, 9 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.7 gene nmagghr, a single gene, synthesis of a cDNA from the total RNA extracted from Natrialba magadii strain ATCC 43099, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli Natrialba magadii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.5.7 purified enzyme in apoform, mixing of 0.5 ml of 6.6 mg/ml protein in 50 mM Tris-HCl, pH 7.5, and 3.5 M NaCl, with 0.5 ml of reservoir solution containing 25% w/v PEG 3350, 0.1 M Tris-HCl, pH 8.5, and 0.2 M lithium sulfate, at 21°C, X-ray diffraction structure determination and analysis at 2.61 A resolution, molecular replacement using the structure of monomeric putative GST from Corynebacterium glutamicum (PDB ID 3M1G) as a search model, model building Natrialba magadii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.7 77000
-
 recombinant N-terminally His6-tagged enzyme, gel filtration Natrialba magadii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii ATCC 43099
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii NBRC 102185
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii DSM 3394
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii CCM 3739
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii NCIMB 2190
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii JCM 8861
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii CIP 104546
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii MS3
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii IAM 13178
-
 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii ATCC 43099
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii NBRC 102185
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii DSM 3394
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii CCM 3739
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii NCIMB 2190
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii JCM 8861
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii CIP 104546
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii MS3
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Natrialba magadii IAM 13178
-
 glutathione disulfide + hydroquinone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.7 Natrialba magadii D3SS28
-
-
1.8.5.7 Natrialba magadii ATCC 43099 D3SS28
-
-
1.8.5.7 Natrialba magadii CCM 3739 D3SS28
-
-
1.8.5.7 Natrialba magadii CIP 104546 D3SS28
-
-
1.8.5.7 Natrialba magadii DSM 3394 D3SS28
-
-
1.8.5.7 Natrialba magadii IAM 13178 D3SS28
-
-
1.8.5.7 Natrialba magadii JCM 8861 D3SS28
-
-
1.8.5.7 Natrialba magadii MS3 D3SS28
-
-
1.8.5.7 Natrialba magadii NBRC 102185 D3SS28
-
-
1.8.5.7 Natrialba magadii NCIMB 2190 D3SS28
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.7 recombinant N-terminally His6-tagged enzyme from Escherichia coli Natrialba magadii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii ?
-
 ?
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii ATCC 43099 ?
-
 ?
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii NBRC 102185 ?
-
 ?
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii DSM 3394 ?
-
 ?
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii CCM 3739 ?
-
 ?
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii NCIMB 2190 ?
-
 ?
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii JCM 8861 ?
-
 ?
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii CIP 104546 ?
-
 ?
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii MS3 ?
-
 ?
1.8.5.7 1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii IAM 13178 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii ATCC 43099 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii NBRC 102185 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii DSM 3394 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii CCM 3739 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii NCIMB 2190 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii JCM 8861 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii CIP 104546 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii MS3 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene
-
 Natrialba magadii IAM 13178 ?
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii ATCC 43099 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii NBRC 102185 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii DSM 3394 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii CCM 3739 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii NCIMB 2190 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii JCM 8861 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii CIP 104546 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii MS3 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii IAM 13178 gamma-glutamylcysteine glutahione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii gamma-glutamylcysteine glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 gamma-glutamylcysteine + ethacrynic acid
-
 Natrialba magadii ?
-
 ?
1.8.5.7 glutathione + 1,4-benzoquinone
-
 Natrialba magadii ?
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii ATCC 43099 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii NBRC 102185 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii DSM 3394 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii CCM 3739 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii NCIMB 2190 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii JCM 8861 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii CIP 104546 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii MS3 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Natrialba magadii IAM 13178 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-hydroxyethyldisulfide
-
 Natrialba magadii ?
-
 ?
1.8.5.7 glutathione + dehydroascorbic acid
-
 Natrialba magadii ?
-
 ?
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii ?
-
-
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii ATCC 43099 ?
-
-
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii NBRC 102185 ?
-
-
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii DSM 3394 ?
-
-
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii CCM 3739 ?
-
-
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii NCIMB 2190 ?
-
-
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii JCM 8861 ?
-
-
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii CIP 104546 ?
-
-
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii MS3 ?
-
-
1.8.5.7 additional information the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate Natrialba magadii IAM 13178 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.8.5.7 ? x * 38000, recombinant N-terminally His6-tagged enzyme, SDS-PAGE Natrialba magadii
1.8.5.7 More structure analysis of enzyme NmGHR, detailed overview Natrialba magadii

Synonyms

EC Number Synonyms Comment Organism
1.8.5.7 nmagghr
-
 Natrialba magadii
1.8.5.7 Nmag_1170
-
 Natrialba magadii
1.8.5.7 NmGHR
-
 Natrialba magadii
1.8.5.7 Xi class glutathione transferase
-
 Natrialba magadii
1.8.5.7 Xi class GST
-
 Natrialba magadii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.5.7 25 40 assay at Natrialba magadii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.7 6.5 8 assay at Natrialba magadii

General Information

EC Number General Information Comment Organism
1.8.5.7 evolution Xi class glutathione transferases (GSTs) are a group, within this large superfamily of enzymes, specifically endowed with glutathione-dependent reductase activity on glutathionyl-hydroquinone. Enzymes belonging to this group are widely distributed in bacteria, fungi, and plants but not in higher eukaryotes. Xi class GSTs are also frequently found in archaea. Multiple alignment analysis of NmGHR in comparison with other Xi class GSTs show that all sequences display several common motifs. All members exhibit a strictly conserved CPWA motif that could play a key role in the binding of GS-(hydro)-quinones. Phylogenetic analysis, members of prokaryotic and eukaryotic Xi class GSTs and their homologous belonging to different GST classes are characterized by the presence of a cysteine residue in the G-site, overview Natrialba magadii
1.8.5.7 additional information enzyme NmGHR displays is enriched in negatively charged residues, which are evenly distributed along the surface of the protein, and is characterized by a peculiar distribution of hydrophobic residues. The thiol binding site (G-site) of the enzyme is well suited for hosting gamma-glutamyl-cysteine. Circular dichroism analysis of NmGHR Natrialba magadii