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Literature summary extracted from

  • Berini, F.; Verce, M.; Ausec, L.; Rosini, E.; Tonin, F.; Pollegioni, L.; Mandic-Mulec, I.
    Isolation and characterization of a heterologously expressed bacterial laccase from the anaerobe Geobacter metallireducens (2018), Appl. Microbiol. Biotechnol., 102, 2425-2439 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 His6-tagged enzyme is heterologously expressed in Escherichia coli Geobacter metallireducens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2,6-dimethoxyphenol substrate inhibition Geobacter metallireducens
1.10.3.2 DMSO 10%, 50% loss of the original activity Geobacter metallireducens
1.10.3.2 Tween-80 1%, more than 50% loss of activity Geobacter metallireducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.01
-
 O2 25°C, pH 5.6 Geobacter metallireducens
1.10.3.2 0.62
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 25°C, pH 5.6 Geobacter metallireducens
1.10.3.2 5.1
-
 2,6-dimethoxyphenol 25°C, pH 5.6 Geobacter metallireducens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 cytoplasm
-
 Geobacter metallireducens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper multicopper oxidase. The apoenzyme is inactive. Incubation with CuSO4 allows a 43fold increase of the specific activity yielding a metallo-enzyme Geobacter metallireducens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 52700
-
 gel filtration Geobacter metallireducens

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Geobacter metallireducens Q39TP1
-
-
1.10.3.2 Geobacter metallireducens GS-15 Q39TP1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Geobacter metallireducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Geobacter metallireducens ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Geobacter metallireducens ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Geobacter metallireducens ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?
1.10.3.2 K4[Fe(CN)6] + O2
-
 Geobacter metallireducens ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Geobacter metallireducens ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Geobacter metallireducens ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Geobacter metallireducens GS-15 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 GMET_RS10855
-
 Geobacter metallireducens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 75
-
-
 Geobacter metallireducens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 6
-
 oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol Geobacter metallireducens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 5 6 good stability in the pH range 5.0-6.0 Geobacter metallireducens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.10.3.2 95.4
-
 2,6-dimethoxyphenol 25°C, pH 5.6 Geobacter metallireducens