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Literature summary extracted from

  • Zhao, L.; Jiang, Y.; Fang, H.; Zhang, H.; Cheng, S.; Rajoka, M.S.R.; Wu, Y.
    Biotransformation of isoeugenol into vanillin using immobilized recombinant cells containing isoeugenol monooxygenase active aggregates (2019), Appl. Biochem. Biotechnol., 189, 448-458 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.88 the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase is efficiently expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

General Stability

EC Number General Stability Organism
1.13.11.88 the relative activity of the immobilized enzyme (with 100 mM glutaraldehyde) maintains over 60% after 7 recycles Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.88 isoeugenol + O2 Pseudomonas putida
-
 vanillin + acetaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.88 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.88 isoeugenol + O2
-
 Pseudomonas putida vanillin + acetaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.88 ? x * 50000, recombinant IEM720-18A fusion protein, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.13.11.88 iem
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.88 25
-
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.88 10.5
-
-
 Pseudomonas putida