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Literature summary extracted from

  • Senavirathne, G.; Lopez, M.A.; Messer, R.; Fishel, R.; Yoder, K.E.
    Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule fluorescence imaging (2018), Anal. Biochem., 556, 78-84 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.3 overexpression in Escherichia coli BL21 with a octa-Histidine (8xHis) tag Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3 a highly active enzyme without nuclease contaminations is purified by Ni-NTA affinity chromatography and size exclusion chromatography for prolonged single-molecule fluorescence imaging Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.13.11.3 PCD
-
 Pseudomonas putida