Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hagedoorn, P.L.
    Steady-state kinetics of the tungsten containing aldehyde ferredoxin oxidoreductases from the hyperthermophilic archaeon Pyrococcus furiosus (2019), J. Biotechnol., 306, 142-148 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.6 NaCl enzyme shows partial substrate inhibition, inhibition is alleviated completely by a 1M NaCl resulting in increased enzyme activity at high substrate concentrations Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.6 D-glyceraldehyde 3-phosphate partial substrate inhibition, inhibition is alleviated completely by a 1M NaCl resulting in increased enzyme activity at high substrate concentrations Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.B2 0.22
-
 formaldehyde pH 8.4, 25°C Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.B2 Pyrococcus furiosus Q8U1K3
-
-
1.2.7.6 Pyrococcus furiosus Q8U3K2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.B2 formaldehyde + H2O + 2 oxidized methyl viologen
-
 Pyrococcus furiosus formate + 2 reduced methyl viologen + 2 H+
-
 ?
1.2.7.6 D-glyceraldehyde 3-phosphate + H2O + 2 oxidized benzyl viologen D-glyceraldehyde 3-phosphate is the only substrate oxidized by GAPOR, and the kinetics of the enzyme are unaffected by the presence of L-glyceraldehyde 3-phosphate Pyrococcus furiosus 3-phospho-D-glycerate + 2 H+ + 2 reduced benzyl viologen
-
 ?
1.2.7.6 additional information substrate glyceraldehyde-3-phosphate degrades at 60°C by non-enzymatic elimination of the phosphate group to methylglyoxal with a half-life of 6.5 min. Methylglyoxal is not a substrate or inhibitor of GAPOR Pyrococcus furiosus ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.2.7.B2 FOR
-
 Pyrococcus furiosus
1.2.7.6 Gor
-
 Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.6 9
-
 at pH 9, the substrate is a divalent anion Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
1.2.7.B2 physiological function enzyme exhibits a significant primary kinetic isotope effect of the apparent Vmax for the deuterated substrate, formaldehyde-d2, which shows that the rate-determining step involves a C-H bond break from the aldehyde Pyrococcus furiosus