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Literature summary extracted from

  • Rowland, J.L.; Niederweis, M.
    A multicopper oxidase is required for copper resistance in Mycobacterium tuberculosis (2013), J. Bacteriol., 195, 3724-3373 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.16.3.1 C35A putative lipidation site is dispensable for MmcO activity: mutation shows only minor impact on enzymatic activity Mycobacterium tuberculosis
1.16.3.1 C486A cysteine 486 is required for MmcO activity. Mutation results in inactive Rv0846c protein which does not protect Mycobacterium tuberculosis against copper stress Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.16.3.1 membrane membrane associated probably by lipidation after export across the inner membrane by the twin-arginine translocation system Mycobacterium tuberculosis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.16.3.1 copper four copper ions are coordinated in two centers comprise the multicopper oxidase active site Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.16.3.1 56000
-
 SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.16.3.1 Fe(II) + 4 H+ + O2 Mycobacterium tuberculosis
-
 Fe(III) + 2 H2O
-
 ?
1.16.3.1 Fe(II) + 4 H+ + O2 Mycobacterium tuberculosis H37Rv
-
 Fe(III) + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.16.3.1 Mycobacterium tuberculosis I6WZK7
-
-
1.16.3.1 Mycobacterium tuberculosis H37Rv I6WZK7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.16.3.1 cell lysate
-
 Mycobacterium tuberculosis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.3.1 Fe(II) + 4 H+ + O2
-
 Mycobacterium tuberculosis Fe(III) + 2 H2O
-
 ?
1.16.3.1 Fe(II) + 4 H+ + O2
-
 Mycobacterium tuberculosis H37Rv Fe(III) + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.16.3.1 ? x * 56000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.16.3.1 MmcO
-
 Mycobacterium tuberculosis
1.16.3.1 multicopper oxidase
-
 Mycobacterium tuberculosis
1.16.3.1 mycobacterial multicopper oxidase
-
 Mycobacterium tuberculosis
1.16.3.1 Rv0846c
-
 Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
1.16.3.1 Mycobacterium tuberculosis expression of the mmcO gene is increased by copper up

General Information

EC Number General Information Comment Organism
1.16.3.1 malfunction deletion of the rv0846c gene increases the susceptibility of Mycobacterium tuberculosis to copper at least 10fold Mycobacterium tuberculosis
1.16.3.1 physiological function multicopper oxidase is required for copper resistance in Mycobacterium tuberculosis Mycobacterium tuberculosis