EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.8.1.4 | Na2S2O4 | the enzyme activity is significantly improved with the addition of Na2S2O4 as a strong deducing reagent | Pseudomonas putida |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.8.1.4 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
EC Number | General Stability | Organism |
---|---|---|
4.8.1.4 | the enzyme completely loses the catalytic activity in 0.1 M glycine-sodium hydroxide buffer (pH 8.4) | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.8.1.4 | 3.25 | - |
3-pyridine aldoxime | at pH 6.0 and 35°C | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.8.1.4 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.8.1.4 | Ni-NTA column chromatography | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.8.1.4 | (1E)-N-hydroxyethanimine | more than 99.9% conversion | Pseudomonas putida | acetonitrile + H2O | - |
? | |
4.8.1.4 | (1E)-N-hydroxyheptan-1-imine | more than 99.9% conversion | Pseudomonas putida | heptanenitrile + H2O | - |
? | |
4.8.1.4 | (1E)-N-hydroxyhexan-1-imine | more than 99.9% conversion | Pseudomonas putida | hexanenitrile + H2O | - |
? | |
4.8.1.4 | (E)-1-(2-chloro-6-fluorophenyl)-N-hydroxymethanimine | 42.7% conversion | Pseudomonas putida | 2-chloro-6-fluorobenzonitrile + H2O | - |
? | |
4.8.1.4 | (E)-N-hydroxy-1-(pyridin-4-yl)methanimine | 12.3% conversion | Pseudomonas putida | pyridine-4-carbonitrile + H2O | - |
? | |
4.8.1.4 | (E)-N-hydroxy-1-(thiophen-2-yl)methanimine | 99.5% conversion | Pseudomonas putida | thiophene-2-carbonitrile + H2O | - |
? | |
4.8.1.4 | (E)-N-hydroxy-1-phenylmethanimine | more than 99.9% conversion | Pseudomonas putida | benzonitrile + H2O | - |
? | |
4.8.1.4 | (Z)-1-(2-bromophenyl)-N-hydroxymethanimine | more than 99.9% conversion | Pseudomonas putida | 2-bromobenzonitrile + H2O | - |
? | |
4.8.1.4 | (Z)-N-hydroxy-1-(pyridin-2-yl)methanimine | 99.8% conversion | Pseudomonas putida | pyridine-2-carbonitrile + H2O | - |
? | |
4.8.1.4 | 2-[(E)-(hydroxyimino)methyl]phenol | 19% conversion | Pseudomonas putida | 2-hydroxybenzonitrile + H2O | - |
? | |
4.8.1.4 | 3-pyridine aldoxime | more than 99.9% conversion | Pseudomonas putida | pyridine-3-carbonitrile + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.8.1.4 | ? | x * 40000, SDS-PAGE | Pseudomonas putida |
4.8.1.4 | ? | x * 41600, calculated from amino acid sequence | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.8.1.4 | OxdF1 | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.8.1.4 | 35 | - |
- |
Pseudomonas putida |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.8.1.4 | 30 | 40 | the enzymatic activity remains above 80% of the maximal activity between 30 and 40°C | Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.8.1.4 | 30 | 35 | the residual activity of recombinant enzyme maintains above 70% of the initial activity after 3 h at 30°C. When the enzyme is incubated at 35°C, about 20% of the initial activity is retained after 1 h. The half-lives of recombinant enzyme are calculated as 3.8 h and 46 min at 30 and 35°C h, respectively | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.8.1.4 | 0.873 | - |
3-pyridine aldoxime | at pH 6.0 and 35°C | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.8.1.4 | 6 | - |
- |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.8.1.4 | FMN | necessarily required | Pseudomonas putida | |
4.8.1.4 | heme | the enzyme carries protoheme IX as the prosthetic group | Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.8.1.4 | 0.2683 | - |
3-pyridine aldoxime | at pH 6.0 and 35°C | Pseudomonas putida |