Literature summary extracted from

Wohlschlager, L.; Csarman, F.; Zrilic, M.; Seiboth, B.; Ludwig, R.
Comparative characterization of glyoxal oxidase from Phanerochaete chrysosporium expressed at high levels in Pichia pastoris and Trichoderma reesei (2021), Enzyme Microb. Technol., 145, 109748 .

Application

EC Number	Application	Comment	Organism
1.2.3.15	synthesis	efficient expression in Pichia pastoris and Trichoderma reesei with subsequent purification by anion exchange and hydrophobic interaction chromatography. Both processes are suitable for the production of the target protein at high levels. GLOX produced in T. reesei carries mainly Man5 glycosylation while the enzyme produced in P. pastoris exhibits the typical high-mannose type N-glycosylation. The enzyme expressed in P. pastoris shows slightly higher specific activities which correlates with the higher copper loading of 65.5 % compared to 51.9 % for the protein from T. reesei	Phanerodontia chrysosporium

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.2.3.15	Phanerodontia chrysosporium	Q01772	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
1.2.3.15	GLOX	-	Phanerodontia chrysosporium

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.2.3.15	6	-	GLOX activity is highly affected by different buffer species	Phanerodontia chrysosporium

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Release 2023.1 (January 2023)