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Literature summary extracted from

  • Rembeza, E.; Boverio, A.; Fraaije, M.W.; Engqvist, M.K.M.
    Discovery of two novel oxidases using a high-throughput activity screen (2022), Chembiochem, 23, e202100510 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.29 expression in Escherichia coli Ralstonia solanacearum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.20 0.0277
-
 1-Dodecanol pH 7.4, 25°C uncultured marine group II/III euryarchaeote KM3_72_H01
1.1.3.29 0.26
-
 N-acetyl-beta-D-glucosamine pH 7.4, 25°C Ralstonia solanacearum
1.1.3.29 0.32
-
 N-acetyl-D-galactosamine pH 7.4, 25°C Ralstonia solanacearum
1.1.3.29 1.4
-
 D-galactosamine pH 7.4, 25°C Ralstonia solanacearum
1.1.3.29 4.5
-
 D-glucosamine pH 7.4, 25°C Ralstonia solanacearum
1.1.3.29 19
-
 chitobiose pH 7.4, 25°C Ralstonia solanacearum
1.1.3.29 65
-
 D-mannosamine pH 7.4, 25°C Ralstonia solanacearum
1.1.3.29 102
-
 D-galactose pH 7.4, 25°C Ralstonia solanacearum
1.1.3.29 118
-
 D-mannose pH 7.4, 25°C Ralstonia solanacearum
1.1.3.29 182
-
 N-acetyl-D-mannosamine pH 7.4, 25°C Ralstonia solanacearum
1.1.3.29 216
-
 D-glucose pH 7.4, 25°C Ralstonia solanacearum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.20 membrane sequence contains a predicted membrane binding helix within the substrate binding domain uncultured marine group II/III euryarchaeote KM3_72_H01 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.20 uncultured marine group II/III euryarchaeote KM3_72_H01 A0A075HNX4
-
-
1.1.3.29 Ralstonia solanacearum A3RXB7
-
-
1.1.3.29 Ralstonia solanacearum UW551 A3RXB7
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.3.20 enzyme is able to refold and regain its activity upon heating up to 65°C uncultured marine group II/III euryarchaeote KM3_72_H01
1.1.3.29 enzyme is able to refold and regain its activity upon heating to up to 80°C Ralstonia solanacearum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.20 0.23
-
 substrate 1-dodecanol, pH 7.4, 25°C uncultured marine group II/III euryarchaeote KM3_72_H01

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.20 1-decanol + O2
-
 uncultured marine group II/III euryarchaeote KM3_72_H01 decanal + H2O2
-
 ?
1.1.3.20 1-dodecanol + O2
-
 uncultured marine group II/III euryarchaeote KM3_72_H01 dodecanal + H2O2
-
 ?
1.1.3.20 1-octanol + O2
-
 uncultured marine group II/III euryarchaeote KM3_72_H01 octanal + H2O2
-
 ?
1.1.3.20 1-tetradecanol + O2
-
 uncultured marine group II/III euryarchaeote KM3_72_H01 tetradecanal + H2O2
-
 ?
1.1.3.20 additional information enzyme is active with substrates having a carbon chain length of six and above, showing the highest activity with 1-dodecanol uncultured marine group II/III euryarchaeote KM3_72_H01 ?
-
-
1.1.3.29 chitobiose + O2
-
 Ralstonia solanacearum ?
-
 ?
1.1.3.29 D-galactosamine + O2
-
 Ralstonia solanacearum ?
-
 ?
1.1.3.29 D-galactosamine + O2
-
 Ralstonia solanacearum UW551 ?
-
 ?
1.1.3.29 D-galactose + O2
-
 Ralstonia solanacearum ?
-
 ?
1.1.3.29 D-glucosamine + O2
-
 Ralstonia solanacearum ?
-
 ?
1.1.3.29 D-glucosamine + O2
-
 Ralstonia solanacearum UW551 ?
-
 ?
1.1.3.29 D-glucose + O2
-
 Ralstonia solanacearum ?
-
 ?
1.1.3.29 D-mannosamine + O2
-
 Ralstonia solanacearum ?
-
 ?
1.1.3.29 D-mannose + O2
-
 Ralstonia solanacearum ?
-
 ?
1.1.3.29 N-acetyl-beta-D-glucosamine + O2
-
 Ralstonia solanacearum N-acetyl-D-glucosaminolactone + H2O2
-
 ?
1.1.3.29 N-acetyl-beta-D-glucosamine + O2
-
 Ralstonia solanacearum UW551 N-acetyl-D-glucosaminolactone + H2O2
-
 ?
1.1.3.29 N-acetyl-D-galactosamine + O2
-
 Ralstonia solanacearum N-acetyl-D-galactosaminolactone + H2O2
-
 ?
1.1.3.29 N-acetyl-D-galactosamine + O2
-
 Ralstonia solanacearum UW551 N-acetyl-D-galactosaminolactone + H2O2
-
 ?
1.1.3.29 N-acetyl-D-mannosamine + O2
-
 Ralstonia solanacearum N-acetyl-D-mannosaminolactone + H2O2
-
 ?
1.1.3.29 N-acetyl-D-mannosamine + O2
-
 Ralstonia solanacearum UW551 N-acetyl-D-mannosaminolactone + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.29 RRSL_02030
-
 Ralstonia solanacearum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.20 80
-
 30 min, complete loss of activity uncultured marine group II/III euryarchaeote KM3_72_H01
1.1.3.29 39
-
 melting temperature, in buffers with pH 6.5 and above Ralstonia solanacearum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.20 8
-
-
 uncultured marine group II/III euryarchaeote KM3_72_H01

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.20 5
-
 almost complete loss of activity uncultured marine group II/III euryarchaeote KM3_72_H01

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.29 FAD absorption spectrum displays maxima at 375 and 445 nm, FAD is bound to residues His64 and Cys123 Ralstonia solanacearum