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Literature summary extracted from

  • Masamura, N.; Ohashi, W.; Tsuge, N.; Imai, S.; Ishii-Nakamura, A.; Hirota, H.; Nagata, T.; Kumagai, H.
    Identification of amino acid residues essential for onion lachrymatory factor synthase activity (2012), Biosci. Biotechnol. Biochem., 76, 447-453 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.12 expressed in Escherichia coli BL-21-Gold cells Allium cepa

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.12 C69S/R71L/K74L inactive Allium cepa
5.3.99.12 E88Q inactive Allium cepa
5.3.99.12 E88Q/D94N inactive Allium cepa
5.3.99.12 R71L inactive Allium cepa
5.3.99.12 T109A/E112Q/Y114F the mutant shows very low activity compared to the wild type enzyme Allium cepa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.12 1-propenylsulfenic acid Allium cepa
-
propanethial S-oxide
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.12 Allium cepa P59082
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.12 1-propenylsulfenic acid
-
Allium cepa propanethial S-oxide
-
?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.12 lachrymatory factor synthase
-
Allium cepa
5.3.99.12 LFS
-
Allium cepa