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Literature summary extracted from

  • Beaupre, B.A.; Forouzesh, D.C.; Moran, G.R.
    Transient-state analysis of porcine dihydropyrimidine dehydrogenase reveals reductive activation by NADPH (2020), Biochemistry, 59, 2419-2431 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.2 C671S mutation of the pyrimidine site candidate general acid, slows the turnover of the enzyme by approximately 60fold for uracil and 1600fold for thymine Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.2 0.00079
-
 thymine wild-type, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.00087
-
 Uracil wild-type, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.0015
-
 Uracil mutant C671S, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.0023
-
 NADPH wild-type, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.0046
-
 thymine wild-type, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.0054
-
 NADPH wild-type, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.0055
-
 Uracil mutant C671S, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.0063
-
 Uracil wild-type, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.0093
-
 NADPH mutant C671S, anaerobic conditions, pH 7.0, 20°C Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Sus scrofa Q28943
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 thymine + NADPH + H+
-
 Sus scrofa 5,6-dihydrothymine + NADP+
-
 ?
1.3.1.2 uracil + NADPH + H+
-
 Sus scrofa 5,6-dihydrouracil + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.2 DPD
-
 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.2 0.00024
-
 thymine mutant C671S, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.015
-
 NADPH mutant C671S, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.015
-
 Uracil mutant C671S, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.3
-
 Uracil mutant C671S, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.39
-
 thymine wild-type, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.42
-
 thymine wild-type, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.65
-
 NADPH wild-type, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.73
-
 Uracil wild-type, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.78
-
 Uracil wild-type, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 0.85
-
 NADPH wild-type, anaerobic conditions, pH 7.0, 20°C Sus scrofa

General Information

EC Number General Information Comment Organism
1.3.1.2 metabolism the reductive activation of DPD results in the reduction of one flavin per dimer consistent with alternating site behavior. During pyrimidine reduction, electron transfer across the flavins and Fe4S4 centers is rapid relative to the other process. The net rate of transmission of electrons from NADPH to the pyrimidine (kcat) must be determined exclusively by the rate of proton transfer from general acid cysteine 671 Sus scrofa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.2 2
-
 NADPH mutant C671S, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 3
-
 Uracil mutant C671S, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 20
-
 Uracil mutant C671S, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 90
-
 thymine wild-type, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 110
-
 Uracil wild-type, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 120
-
 NADPH wild-type, aerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 360
-
 NADPH wild-type, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 500
-
 thymine wild-type, anaerobic conditions, pH 7.0, 20°C Sus scrofa
1.3.1.2 900
-
 Uracil wild-type, anaerobic conditions, pH 7.0, 20°C Sus scrofa