EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.13 | expression in Escherichia coli | Phanerodontia chrysosporium |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.3.13 | structure to 2.6 A resolution. The catalytic center is a solvent-inaccessible cavity located at the re side of the flavin cofactor. Its small size explains the observed preference for methanol and ethanol as best substrates | Phanerodontia chrysosporium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.3.13 | F101N | with enlarged catalytic cavity, increase in activity with substrates 1-propanol, glycerol, (R)-1,2-propanediol | Phanerodontia chrysosporium |
1.1.3.13 | F101S | with enlarged catalytic cavity, retains a high degree of thermostability | Phanerodontia chrysosporium |
1.1.3.13 | M103S | with enlarged catalytic cavity, increase in activity with substrates 1-propanol, glycerol, (R)-1,2-propanediol | Phanerodontia chrysosporium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.13 | 1 | - |
ethanol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1 | - |
ethanol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 2 | - |
methanol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 2 | - |
methanol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 2 | - |
methanol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 2 | - |
1-propanol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 4 | - |
1-propanol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 11 | - |
ethanol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 11 | - |
1-propanol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 14 | - |
methanol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 15 | - |
ethanol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 45 | - |
1-propanol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 110 | - |
(R)-1,2-Propanediol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 110 | - |
(R)-1,2-Propanediol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1200 | - |
(R)-1,2-Propanediol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1600 | - |
(R)-1,2-Propanediol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.13 | Phanerodontia chrysosporium | T2M2J4 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.13 | (R)-1,2-propanediol + O2 | - |
Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
1.1.3.13 | 1-propanol + O2 | - |
Phanerodontia chrysosporium | propanal + H2O2 | - |
? | |
1.1.3.13 | ethanol + O2 | - |
Phanerodontia chrysosporium | acetaldehyde + H2O2 | - |
? | |
1.1.3.13 | methanol + O2 | - |
Phanerodontia chrysosporium | formaldehyde + H2O2 | - |
? | |
1.1.3.13 | additional information | poor substrate: glycerol | Phanerodontia chrysosporium | ? | - |
- |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.13 | 51 | - |
melting temperature of mutant F101S, 5 mM phoshate buffer pH 7.5 | Phanerodontia chrysosporium |
1.1.3.13 | 52 | - |
melting temperature of mutant F101N, 5 mM phoshate buffer pH 7.5 | Phanerodontia chrysosporium |
1.1.3.13 | 58 | - |
melting temperature of wild-type, 5 mM phoshate buffer pH 7.5 | Phanerodontia chrysosporium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.13 | 0.4 | - |
(R)-1,2-Propanediol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1 | - |
ethanol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1 | - |
methanol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1 | - |
1-propanol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 4 | - |
methanol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 4 | - |
(R)-1,2-Propanediol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 5 | - |
(R)-1,2-Propanediol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 5 | - |
ethanol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 7 | - |
1-propanol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 10 | - |
ethanol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 10 | - |
methanol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 11 | - |
1-propanol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 12 | - |
1-propanol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 13 | - |
(R)-1,2-Propanediol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 18 | - |
methanol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 19 | - |
ethanol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.13 | 9 | - |
- |
Phanerodontia chrysosporium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.13 | 7 | 10 | more than 75% of maximum activity | Phanerodontia chrysosporium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.13 | FAD | - |
Phanerodontia chrysosporium |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.13 | 0.003 | - |
(R)-1,2-Propanediol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 0.004 | - |
(R)-1,2-Propanediol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 0.011 | - |
(R)-1,2-Propanediol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 0.036 | - |
(R)-1,2-Propanediol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 0.24 | - |
1-propanol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 0.5 | - |
methanol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 0.5 | - |
1-propanol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 0.71 | - |
methanol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 0.91 | - |
ethanol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1 | - |
ethanol | mutant M103S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1.1 | - |
1-propanol | mutant F101N, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1.3 | - |
ethanol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 1.8 | - |
1-propanol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 2 | - |
methanol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 5 | - |
ethanol | mutant F101S, pH 7.5, 23°C | Phanerodontia chrysosporium | |
1.1.3.13 | 9 | - |
methanol | wild-type, pH 7.5, 23°C | Phanerodontia chrysosporium |