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Literature summary extracted from

  • Nguyen, Q.T.; Romero, E.; Dijkman, W.P.; de Vasconcellos, S.P.; Binda, C.; Mattevi, A.; Fraaije, M.W.
    Structure-based engineering of Phanerochaete chrysosporium alcohol oxidase for enhanced oxidative power toward glycerol (2018), Biochemistry, 57, 6209-6218 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.13 expression in Escherichia coli Phanerodontia chrysosporium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.13 structure to 2.6 A resolution. The catalytic center is a solvent-inaccessible cavity located at the re side of the flavin cofactor. Its small size explains the observed preference for methanol and ethanol as best substrates Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.13 F101N with enlarged catalytic cavity, increase in activity with substrates 1-propanol, glycerol, (R)-1,2-propanediol Phanerodontia chrysosporium
1.1.3.13 F101S with enlarged catalytic cavity, retains a high degree of thermostability Phanerodontia chrysosporium
1.1.3.13 M103S with enlarged catalytic cavity, increase in activity with substrates 1-propanol, glycerol, (R)-1,2-propanediol Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 1
-
ethanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1
-
ethanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 2
-
methanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 2
-
methanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 2
-
methanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 2
-
1-propanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 4
-
1-propanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 11
-
ethanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 11
-
1-propanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 14
-
methanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 15
-
ethanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 45
-
1-propanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 110
-
(R)-1,2-Propanediol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 110
-
(R)-1,2-Propanediol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1200
-
(R)-1,2-Propanediol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1600
-
(R)-1,2-Propanediol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Phanerodontia chrysosporium T2M2J4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 (R)-1,2-propanediol + O2
-
Phanerodontia chrysosporium ? + H2O2
-
?
1.1.3.13 1-propanol + O2
-
Phanerodontia chrysosporium propanal + H2O2
-
?
1.1.3.13 ethanol + O2
-
Phanerodontia chrysosporium acetaldehyde + H2O2
-
?
1.1.3.13 methanol + O2
-
Phanerodontia chrysosporium formaldehyde + H2O2
-
?
1.1.3.13 additional information poor substrate: glycerol Phanerodontia chrysosporium ?
-
-

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 51
-
melting temperature of mutant F101S, 5 mM phoshate buffer pH 7.5 Phanerodontia chrysosporium
1.1.3.13 52
-
melting temperature of mutant F101N, 5 mM phoshate buffer pH 7.5 Phanerodontia chrysosporium
1.1.3.13 58
-
melting temperature of wild-type, 5 mM phoshate buffer pH 7.5 Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.13 0.4
-
(R)-1,2-Propanediol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1
-
ethanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1
-
methanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1
-
1-propanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 4
-
methanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 4
-
(R)-1,2-Propanediol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 5
-
(R)-1,2-Propanediol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 5
-
ethanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 7
-
1-propanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 10
-
ethanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 10
-
methanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 11
-
1-propanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 12
-
1-propanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 13
-
(R)-1,2-Propanediol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 18
-
methanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 19
-
ethanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 9
-
-
Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.13 7 10 more than 75% of maximum activity Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD
-
Phanerodontia chrysosporium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.13 0.003
-
(R)-1,2-Propanediol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 0.004
-
(R)-1,2-Propanediol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 0.011
-
(R)-1,2-Propanediol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 0.036
-
(R)-1,2-Propanediol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 0.24
-
1-propanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 0.5
-
methanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 0.5
-
1-propanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 0.71
-
methanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 0.91
-
ethanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1
-
ethanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1.1
-
1-propanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1.3
-
ethanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 1.8
-
1-propanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 2
-
methanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 5
-
ethanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1.3.13 9
-
methanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium