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Literature summary extracted from

  • Ouedraogo, D.; Ball, J.; Iyer, A.; Reis, R.A.G.; Vodovoz, M.; Gadda, G.
    Amine oxidation by D-arginine dehydrogenase in Pseudomonas aeruginosa (2017), Arch. Biochem. Biophys., 632, 192-201 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.99.6 structure without added D-amino acids, to 1.06 A resolution, and in presence of D-arginine and D-histidine Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.99.6 0.06
-
 D-arginine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.26
-
 D-Lysine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.8
-
 D-tyrosine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 10
-
 D-methionine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 11
-
 D-phenylalanine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 11
-
 D-histidine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 12
-
 D-leucine pH 8.7, 25°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.4.99.6 Pseudomonas aeruginosa Q9HXE3
-
-
1.4.99.6 Pseudomonas aeruginosa DSM 22644 Q9HXE3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.99.6 D-alanine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa pyruvate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-alanine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa DSM 22644 pyruvate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-arginine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-arginine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa DSM 22644 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-asparagine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 2-oxobutanedioate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-asparagine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa DSM 22644 2-oxobutanedioate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-glutamine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 5-amino-2,5-dioxopentanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-glutamine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa DSM 22644 5-amino-2,5-dioxopentanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-histidine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-isoleucine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 3-methyl-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-leucine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 4-methyl-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-lysine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 7-amino-2-oxoheptanoic acid + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-methionine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-phenylalanine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 2-oxo-3-phenylpropanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-proline + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa DELTA1-pyrroline-2-carboxylate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-serine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 3-hydroxy-2-oxopropanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-threonine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 3-hydroxy-2-oxobutanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-tryptophan + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 3-indole-2-oxopropanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-tyrosine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa (4-hydroxyphenyl)pyruvate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-valine + phenazine methosulfate + H2O
-
 Pseudomonas aeruginosa 2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 additional information no substrates: aspartae, glutamate Pseudomonas aeruginosa ?
-
-
1.4.99.6 additional information no substrates: aspartae, glutamate Pseudomonas aeruginosa DSM 22644 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.4.99.6 DADH
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.99.6 6.4
-
 D-leucine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 23
-
 D-tyrosine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 35
-
 D-histidine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 75
-
 D-phenylalanine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 141
-
 D-Lysine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 154
-
 D-methionine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 204
-
 D-arginine pH 8.7, 25°C Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.99.6 FAD contains a noncovalently linked FAD Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
1.4.99.6 metabolism DADH catalyzes an asynchronous CH and NH bond cleavage via a hydride transfer mechanism. The enzyme follows a ping-pong bibi mechanism. The shape and flexibility of loop L1 in the active site of DADH are important for substrate capture and broad substrate specificity Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.99.6 0.00075
-
 D-threonine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.0038
-
 D-serine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.016
-
 D-asparagine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.041
-
 D-alanine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.047
-
 D-valine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.186
-
 D-glutamine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.195
-
 D-isoleucine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.245
-
 D-tryptophan pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.42
-
 D-proline pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.515
-
 D-leucine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 3.14
-
 D-histidine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 6.9
-
 D-phenylalanine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 14.8
-
 D-methionine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 27.6
-
 D-tyrosine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 530
-
 D-Lysine pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 3400
-
 D-arginine pH 8.7, 25°C Pseudomonas aeruginosa