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Literature summary extracted from

  • Yang, D.D.; de Billerbeck, G.M.; Zhang, J.J.; Rosenzweig, F.; Francois, J.M.
    Deciphering the origin, evolution, and physiological function of the subtelomeric aryl-alcohol dehydrogenase gene family in the yeast Saccharomyces cerevisiae (2018), Appl. Environ. Microbiol., 84, e01553-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.91 gene AAD1, phylogenetic analysis, subcloning of tagged PcAAD4 into the multicopy YEplac195 plasmid under the control of the strong constitutive PGK1 promoter and recombinant expression in Saccharomyces wild-type strain BY4741, activity in BY4741 cell crude extracts expressing PcAad1p is 2fold higher than that of the blank-plasmid control Phanerodontia chrysosporium
1.1.1.91 gene AAD14, phylogenetic analysis, subcloning of tagged ScAAD14 into the multicopy YEplac195 plasmid under the control of the strong constitutive PGK1 promoter and recombinant expression in Saccharomyces wild-type strain BY4741, the enzyme activity in cells expressing ScAd14p does not significantly differ from control Saccharomyces cerevisiae
1.1.1.91 gene AAD4, phylogenetic analysis, subcloning of tagged ScAAD4 into the multicopy YEplac195 plasmid under the control of the strong constitutive PGK1 promoter and recombinant expression in Saccharomyces wild-type strain BY4741, the enzyme activity in cells expressing ScAd4p does not significantly differ from control Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.91 additional information construction of a 46-residue N-terminal truncation mutant Saccharomyces cerevisiae
1.1.1.91 Y76C site-directed mutagenesis Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.91 0.012
-
 3,4-dimethoxybenzaldehyde pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium
1.1.1.91 1.7
-
 benzaldehyde pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium
1.1.1.91 3.4
-
 cinnamaldehyde pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.91 Phanerodontia chrysosporium
-
-
-
1.1.1.91 Saccharomyces cerevisiae P42884
-
-
1.1.1.91 Saccharomyces cerevisiae Q07747
-
-
1.1.1.91 Saccharomyces cerevisiae ATCC 204508 P42884
-
-
1.1.1.91 Saccharomyces cerevisiae ATCC 204508 Q07747
-
-
1.1.1.91 Saccharomyces cerevisiae BY4741 P42884
-
-
1.1.1.91 Saccharomyces cerevisiae BY4741 Q07747
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.91 3,4-dimethoxybenzaldehyde + NADPH + H+
-
 Phanerodontia chrysosporium 3,4-dimethoxybenzol + NADP+
-
 r
1.1.1.91 3,4-dimethoxybenzaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae 3,4-dimethoxybenzol + NADP+
-
 r
1.1.1.91 3,4-dimethoxybenzaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae BY4741 3,4-dimethoxybenzol + NADP+
-
 r
1.1.1.91 3,4-dimethoxybenzaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ATCC 204508 3,4-dimethoxybenzol + NADP+
-
 r
1.1.1.91 4-nitrobenzaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae 4-nitrobenzylalcohol + NADP+
-
 r
1.1.1.91 4-nitrobenzaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae BY4741 4-nitrobenzylalcohol + NADP+
-
 r
1.1.1.91 4-nitrobenzaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ATCC 204508 4-nitrobenzylalcohol + NADP+
-
 r
1.1.1.91 benzaldehyde + NADPH + H+
-
 Phanerodontia chrysosporium benzyl alcohol + NADP+
-
 r
1.1.1.91 cinnamaldehyde + NADPH + H+
-
 Phanerodontia chrysosporium cinnamoyl alcohol + NADP+
-
 r
1.1.1.91 heptanal + NADPH + H+
-
 Phanerodontia chrysosporium heptanol + NADP+
-
 r
1.1.1.91 heptanal + NADPH + H+
-
 Saccharomyces cerevisiae heptanol + NADP+
-
 r
1.1.1.91 heptanal + NADPH + H+
-
 Saccharomyces cerevisiae BY4741 heptanol + NADP+
-
 r
1.1.1.91 heptanal + NADPH + H+
-
 Saccharomyces cerevisiae ATCC 204508 heptanol + NADP+
-
 r
1.1.1.91 hexanal + NADPH + H+
-
 Phanerodontia chrysosporium hexanol + NADP+
-
 r
1.1.1.91 hexanal + NADPH + H+
-
 Saccharomyces cerevisiae hexanol + NADP+
-
 r
1.1.1.91 hexanal + NADPH + H+
-
 Saccharomyces cerevisiae BY4741 hexanol + NADP+
-
 r
1.1.1.91 hexanal + NADPH + H+
-
 Saccharomyces cerevisiae ATCC 204508 hexanol + NADP+
-
 r
1.1.1.91 additional information substrate specificity, overview. PcAad1p is active on a broader spectrum of aryl-aldehyde substrates. PcAad1p can also act as an oxidase against several aliphatic and aromatic alcohols (at pH 10.3, with NADP+ as a cofactor) Phanerodontia chrysosporium ?
-
-
1.1.1.91 additional information substrate specificity, overview. ScAad14p exhibits its highest activity on aryl-aldehydes substituted at position 2. No activity with cinnamaldehyde Saccharomyces cerevisiae ?
-
-
1.1.1.91 additional information substrate specificity, overview. ScAad4p is more active on aryl-aldehyde-bearing substituents at positions 3, 4, and 5, or even those bearing double and triple substitutions on the aromatic ring, while ScAad4p is inactive on aryl-aldehydes that have position 2 substitutions. No activity with 4-nitrobenzaldehyde Saccharomyces cerevisiae ?
-
-
1.1.1.91 additional information substrate specificity, overview. ScAad14p exhibits its highest activity on aryl-aldehydes substituted at position 2. No activity with cinnamaldehyde Saccharomyces cerevisiae BY4741 ?
-
-
1.1.1.91 additional information substrate specificity, overview. ScAad4p is more active on aryl-aldehyde-bearing substituents at positions 3, 4, and 5, or even those bearing double and triple substitutions on the aromatic ring, while ScAad4p is inactive on aryl-aldehydes that have position 2 substitutions. No activity with 4-nitrobenzaldehyde Saccharomyces cerevisiae BY4741 ?
-
-
1.1.1.91 additional information substrate specificity, overview. ScAad14p exhibits its highest activity on aryl-aldehydes substituted at position 2. No activity with cinnamaldehyde Saccharomyces cerevisiae ATCC 204508 ?
-
-
1.1.1.91 additional information substrate specificity, overview. ScAad4p is more active on aryl-aldehyde-bearing substituents at positions 3, 4, and 5, or even those bearing double and triple substitutions on the aromatic ring, while ScAad4p is inactive on aryl-aldehydes that have position 2 substitutions. No activity with 4-nitrobenzaldehyde Saccharomyces cerevisiae ATCC 204508 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.91 AAD1
-
 Phanerodontia chrysosporium
1.1.1.91 AAD14
-
 Saccharomyces cerevisiae
1.1.1.91 Aad14p
-
 Saccharomyces cerevisiae
1.1.1.91 Aad1p
-
 Phanerodontia chrysosporium
1.1.1.91 AAD4
-
 Saccharomyces cerevisiae
1.1.1.91 Aad4p
-
 Saccharomyces cerevisiae
1.1.1.91 aryl-alcohol dehydrogenase
-
 Phanerodontia chrysosporium
1.1.1.91 aryl-alcohol dehydrogenase
-
 Saccharomyces cerevisiae
1.1.1.91 PcAad1p
-
 Phanerodontia chrysosporium
1.1.1.91 ScAad14p protein
-
 Saccharomyces cerevisiae
1.1.1.91 ScAad4p protein
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.91 0.37
-
 4-nitrobenzaldehyde pH 6.1, temperature not specified in the publication Saccharomyces cerevisiae
1.1.1.91 0.77
-
 Heptanal pH 6.1, temperature not specified in the publication Saccharomyces cerevisiae
1.1.1.91 0.83
-
 hexanal pH 6.1, temperature not specified in the publication Saccharomyces cerevisiae
1.1.1.91 1.07
-
 Heptanal pH 6.1, temperature not specified in the publication Saccharomyces cerevisiae
1.1.1.91 2.3
-
 Heptanal pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium
1.1.1.91 2.37
-
 3,4-dimethoxybenzaldehyde pH 6.1, temperature not specified in the publication Saccharomyces cerevisiae
1.1.1.91 2.87
-
 hexanal pH 6.1, temperature not specified in the publication Saccharomyces cerevisiae
1.1.1.91 4.12
-
 hexanal pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium
1.1.1.91 7.17
-
 benzaldehyde pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium
1.1.1.91 8.83
-
 3,4-dimethoxybenzaldehyde pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium
1.1.1.91 11.17
-
 cinnamaldehyde pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.91 6.1
-
 assay at Phanerodontia chrysosporium
1.1.1.91 6.1
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.91 additional information no activity with NADH Saccharomyces cerevisiae
1.1.1.91 additional information the enzyme is active with NADPH and NADH Phanerodontia chrysosporium
1.1.1.91 NADH
-
 Phanerodontia chrysosporium
1.1.1.91 NADP+
-
 Saccharomyces cerevisiae
1.1.1.91 NADPH
-
 Saccharomyces cerevisiae
1.1.1.91 NADPH preferred cofactor Phanerodontia chrysosporium

Expression

EC Number Organism Comment Expression
1.1.1.91 Saccharomyces cerevisiae yeast AAD gene expression is not induced by aryl-aldehydes additional information

General Information

EC Number General Information Comment Organism
1.1.1.91 evolution phylogenetic analysis Phanerodontia chrysosporium
1.1.1.91 evolution function and phylogeny of the yeast Aad gene family reveals that only two genes of the family (AAD4 and AAD14) encode enzymatic activities on aliphatic and aryl-aldehydes, whereas the other five putative members are being pseudogenized Saccharomyces cerevisiae
1.1.1.91 evolution natural single-base-pair substitutions, truncations, and deletions in the AAD gene family of yeast S288C, overview. Phylogenetic analysis Saccharomyces cerevisiae
1.1.1.91 malfunction natural single-base-pair substitutions, truncations, and deletions in the AAD gene family of yeast S288C, overview. No mutations in Asd14 detected. Yeast AAD overexpression does not increase yeast resistance to aryl-aldehyde compounds Saccharomyces cerevisiae
1.1.1.91 malfunction natural single-base-pair substitutions, truncations, and deletions in the AAD gene family of yeast S288C, overview. Yeast AAD overexpression does not increase yeast resistance to aryl-aldehyde compounds Saccharomyces cerevisiae
1.1.1.91 physiological function Phanerochaete chrysosporium Aad1p can reduce aryl-aldehyde derivatives to their corresponding less-toxic alcohol forms Phanerodontia chrysosporium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.91 3.29
-
 cinnamaldehyde pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium
1.1.1.91 4.2
-
 benzaldehyde pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium
1.1.1.91 735.8
-
 3,4-dimethoxybenzaldehyde pH 6.1, temperature not specified in the publication Phanerodontia chrysosporium