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Literature summary extracted from

  • Zimmerling, J.; Oelschlaegel, M.; Grossmann, C.; Voitel, M.; Schloemann, M.; Tischler, D.
    Biochemical characterization of phenylacetaldehyde dehydrogenases from styrene-degrading soil bacteria (2021), Appl. Biochem. Biotechnol., 193, 650-667 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.39 expressed in Escherichia coli BL21(DE3) pLysS cells Gordonia rubripertincta
1.2.1.39 expressed in Escherichia coli BL21(DE3) pLysS cells Rhodococcus opacus
1.2.1.39 expressed in Escherichia coli BL21(DE3) pLysS cells Escherichia coli
1.2.1.39 expressed in Escherichia coli BL21(DE3) pLysS cells Sphingopyxis fribergensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.39 0.022
-
 phenylacetaldehyde with NAD+ as cosubstrate, at pH 7.7, temperature not specified in the publication Sphingopyxis fribergensis
1.2.1.39 0.04
-
 phenylacetaldehyde with NAD+ as cosubstrate, at pH 7.7, temperature not specified in the publication Rhodococcus opacus
1.2.1.39 0.06
-
 phenylacetaldehyde with NAD+ as cosubstrate, at pH 7.7, temperature not specified in the publication Gordonia rubripertincta
1.2.1.39 0.08
-
 NAD+ with phenylacetaldehyde as cosubstrate, at pH 7.7, temperature not specified in the publication Gordonia rubripertincta
1.2.1.39 0.38
-
 NAD+ with phenylacetaldehyde as cosubstrate, at pH 7.7, temperature not specified in the publication Rhodococcus opacus
1.2.1.39 0.5
-
 NAD+ with phenylacetaldehyde as cosubstrate, at pH 7.7, temperature not specified in the publication Sphingopyxis fribergensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.39 phenylacetaldehyde + NAD+ + H2O Gordonia rubripertincta
-
 phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O Rhodococcus opacus
-
 phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O Escherichia coli
-
 phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O Sphingopyxis fribergensis
-
 phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O Gordonia rubripertincta CWB2
-
 phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O Rhodococcus opacus 1CP
-
 phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O Sphingopyxis fribergensis Kp5.2
-
 phenylacetate + NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.39 Escherichia coli P80668
-
-
1.2.1.39 Gordonia rubripertincta
-
-
-
1.2.1.39 Gordonia rubripertincta CWB2
-
-
-
1.2.1.39 Rhodococcus opacus
-
-
-
1.2.1.39 Rhodococcus opacus 1CP
-
-
-
1.2.1.39 Sphingopyxis fribergensis A0A0A7PB40
-
-
1.2.1.39 Sphingopyxis fribergensis Kp5.2 A0A0A7PB40
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.39 Ni-affinity column chromatography Gordonia rubripertincta
1.2.1.39 Ni-affinity column chromatography Rhodococcus opacus
1.2.1.39 Ni-affinity column chromatography Escherichia coli
1.2.1.39 Ni-affinity column chromatography Sphingopyxis fribergensis

Storage Stability

EC Number Storage Stability Organism
1.2.1.39 -20°C, phosphate buffer (pH 7.7), 99% (v/v) glycerol, 76 days, 44.3% residual activity Sphingopyxis fribergensis
1.2.1.39 -20°C, phosphate buffer (pH 7.7), 99% (v/v) glycerol, 93 days, 92.3% residual activity Gordonia rubripertincta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.39 1-methylphenylacetaldehyde + NAD+ + H2O lowest activity Gordonia rubripertincta 1-methylphenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 1-methylphenylacetaldehyde + NAD+ + H2O lowest activity Rhodococcus opacus 1-methylphenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 1-methylphenylacetaldehyde + NAD+ + H2O lowest activity Escherichia coli 1-methylphenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 1-methylphenylacetaldehyde + NAD+ + H2O lowest activity Sphingopyxis fribergensis 1-methylphenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 1-methylphenylacetaldehyde + NAD+ + H2O lowest activity Gordonia rubripertincta CWB2 1-methylphenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 1-methylphenylacetaldehyde + NAD+ + H2O lowest activity Rhodococcus opacus 1CP 1-methylphenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 1-methylphenylacetaldehyde + NAD+ + H2O lowest activity Sphingopyxis fribergensis Kp5.2 1-methylphenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-chlorophenylacetaldehyde + NAD+ + H2O
-
 Gordonia rubripertincta 4-chlorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-chlorophenylacetaldehyde + NAD+ + H2O
-
 Rhodococcus opacus 4-chlorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-chlorophenylacetaldehyde + NAD+ + H2O
-
 Escherichia coli 4-chlorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-chlorophenylacetaldehyde + NAD+ + H2O highest activity Sphingopyxis fribergensis 4-chlorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-chlorophenylacetaldehyde + NAD+ + H2O
-
 Gordonia rubripertincta CWB2 4-chlorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-chlorophenylacetaldehyde + NAD+ + H2O
-
 Rhodococcus opacus 1CP 4-chlorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-fluorophenylacetaldehyde + NAD+ + H2O
-
 Rhodococcus opacus 4-fluorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-fluorophenylacetaldehyde + NAD+ + H2O
-
 Escherichia coli 4-fluorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-fluorophenylacetaldehyde + NAD+ + H2O highest activity Gordonia rubripertincta 4-fluorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-fluorophenylacetaldehyde + NAD+ + H2O high activity Sphingopyxis fribergensis 4-fluorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 4-fluorophenylacetaldehyde + NAD+ + H2O highest activity Gordonia rubripertincta CWB2 4-fluorophenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 additional information no activity with phenazine methosulfate Escherichia coli ?
-
-
1.2.1.39 additional information no activity with phenazine methosulfate Sphingopyxis fribergensis ?
-
-
1.2.1.39 additional information no activity with phenazine methosulfate Sphingopyxis fribergensis Kp5.2 ?
-
-
1.2.1.39 phenylacetaldehyde + NAD+ + H2O
-
 Gordonia rubripertincta phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O
-
 Rhodococcus opacus phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O
-
 Escherichia coli phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O
-
 Sphingopyxis fribergensis phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O highest activity Rhodococcus opacus phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O highest activity Escherichia coli phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O highest activity Sphingopyxis fribergensis phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O
-
 Gordonia rubripertincta CWB2 phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O
-
 Rhodococcus opacus 1CP phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O highest activity Rhodococcus opacus 1CP phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O
-
 Sphingopyxis fribergensis Kp5.2 phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O highest activity Sphingopyxis fribergensis Kp5.2 phenylacetate + NADH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NADP+ + H2O 10.6% activity compared to NAD+ Escherichia coli phenylacetate + NADPH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NADP+ + H2O 29.5% activity compared to NAD+ Rhodococcus opacus phenylacetate + NADPH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NADP+ + H2O 31.7% activity compared to NAD+ Gordonia rubripertincta phenylacetate + NADPH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NADP+ + H2O 4% activity compared to NAD+ Sphingopyxis fribergensis phenylacetate + NADPH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NADP+ + H2O 31.7% activity compared to NAD+ Gordonia rubripertincta CWB2 phenylacetate + NADPH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NADP+ + H2O 29.5% activity compared to NAD+ Rhodococcus opacus 1CP phenylacetate + NADPH + 2 H+
-
 ?
1.2.1.39 phenylacetaldehyde + NADP+ + H2O 4% activity compared to NAD+ Sphingopyxis fribergensis Kp5.2 phenylacetate + NADPH + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.39 feaB
-
 Escherichia coli
1.2.1.39 feaB
-
 Sphingopyxis fribergensis
1.2.1.39 PAD
-
 Gordonia rubripertincta
1.2.1.39 PAD
-
 Rhodococcus opacus
1.2.1.39 PAD
-
 Escherichia coli
1.2.1.39 PAD
-
 Sphingopyxis fribergensis
1.2.1.39 styD
-
 Gordonia rubripertincta
1.2.1.39 styD
-
 Rhodococcus opacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.39
-
 50 after 30 min at 0-50°C in steps of 5°C, the residual activity after this treatment is compared with the initial activity of the enzyme preparation Sphingopyxis fribergensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.39 NAD+ preferred co-substrate Gordonia rubripertincta
1.2.1.39 NAD+ preferred co-substrate Rhodococcus opacus
1.2.1.39 NAD+ preferred co-substrate Escherichia coli
1.2.1.39 NAD+ preferred co-substrate Sphingopyxis fribergensis
1.2.1.39 NADP+
-
 Gordonia rubripertincta
1.2.1.39 NADP+
-
 Rhodococcus opacus
1.2.1.39 NADP+
-
 Escherichia coli
1.2.1.39 NADP+
-
 Sphingopyxis fribergensis