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Literature summary extracted from

  • Jiang, W.; Wang, Y.L.; Fang, B.S.
    Resolution mechanism and characterization of an ammonium chloride-tolerant, high-thermostable, and salt-tolerant phenylalanine dehydrogenase from Bacillus halodurans (2018), Appl. Biochem. Biotechnol., 186, 789-804 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.20 NaCl 2 h in 3 M NaCl at 60 °C, the residual activity is 1.8fold higher than that of the control without NaCl Halalkalibacterium halodurans
1.4.1.20 NH4+ enzyme tolerates high concentrations of ammonium chloride and its activity can also be enhanced by high concentration of ammonium salts Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Halalkalibacterium halodurans Q9KG94
-
-
1.4.1.20 Halalkalibacterium halodurans DSM 18197 Q9KG94
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.20 3.6
-
 oxidative deamination, substrate phnylpyruvate, pH 10.4, 25°C Halalkalibacterium halodurans
1.4.1.20 26.6
-
 reductive amination, substrate phenylpyruvate, pH 10.4, 25°C Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 L-phenylalanine + H2O + NAD+
-
 Halalkalibacterium halodurans phenylpyruvate + NH3 + NADH + H+
-
 ?
1.4.1.20 L-phenylalanine + H2O + NAD+
-
 Halalkalibacterium halodurans DSM 18197 phenylpyruvate + NH3 + NADH + H+
-
 ?
1.4.1.20 phenylpyruvate + NH3 + NADH + H+
-
 Halalkalibacterium halodurans L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 phenylpyruvate + NH3 + NADH + H+
-
 Halalkalibacterium halodurans DSM 18197 L-phenylalanine + H2O + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.20 ? x * 41800, calculated from sequence Halalkalibacterium halodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.20 60
-
 reductive amination Halalkalibacterium halodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.20 37 60 2 h incubation, pH 7.0, enzyme retaines approximately 80% of maximum activity Halalkalibacterium halodurans
1.4.1.20 70
-
 2 h, less than 30% residual activity Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.20 7
-
-
 Halalkalibacterium halodurans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.20 7 8.5 more than 70% of maximum activity Halalkalibacterium halodurans