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Literature summary extracted from

  • Singh, M.; Tiwari, P.; Arora, G.; Agarwal, S.; Kidwai, S.; Singh, R.
    Establishing Virulence Associated Polyphosphate Kinase 2 as a drug target for Mycobacterium tuberculosis (2016), Sci. Rep., 6, 26900 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1
-
 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 F125A the proteins has significantly reduced activity as compared to wild type protein Mycobacterium tuberculosis
2.7.4.1 G72A the activity of MBP-G72APPK-2 and wild type proteins are similar Mycobacterium tuberculosis
2.7.4.1 W129A the proteins has significantly reduced activity as compared to wild type protein Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 NSC-30205 0.1 mM, inhibits more than 80%, cytotoxic to THP-1 macrophages with TC50 value 0.01 mM Mycobacterium tuberculosis
2.7.4.1 NSC-345647 0.1 mM, inhibits more than 80%, cytotoxic to THP-1 macrophages with TC50 value 0.005 mM Mycobacterium tuberculosis
2.7.4.1 NSC-35676 0.1 mM, inhibits more than 80%, non-cytotoxic to THP-1 cells even at 0.05 mM concentration Mycobacterium tuberculosis
2.7.4.1 NSC-9037 0.1 mM, inhibits more than 80%, non-cytotoxic to THP-1 cells even at 0.05 mM concentration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ADP + (phosphate)n+1 Mycobacterium tuberculosis
-
 ATP + (phosphate)n
-
 ?
2.7.4.1 ADP + (phosphate)n+1 Mycobacterium tuberculosis H37Rv
-
 ATP + (phosphate)n
-
 ?
2.7.4.1 ADP + (phosphate)n+1 Mycobacterium tuberculosis ATCC 25618
-
 ATP + (phosphate)n
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Mycobacterium tuberculosis O05877
-
-
2.7.4.1 Mycobacterium tuberculosis H37Rv O05877
-
-
2.7.4.34 Mycobacterium tuberculosis O05877
-
-
2.7.4.34 Mycobacterium tuberculosis ATCC 25618 O05877
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + (phosphate)3 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis ATP + (phosphate)2
-
 ?
2.7.4.1 ADP + (phosphate)3 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis H37Rv ATP + (phosphate)2
-
 ?
2.7.4.1 ADP + (phosphate)3 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis ATCC 25618 ATP + (phosphate)2
-
 ?
2.7.4.1 ADP + (phosphate)n+1
-
 Mycobacterium tuberculosis ATP + (phosphate)n
-
 ?
2.7.4.1 ADP + (phosphate)n+1 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis ATP + (phosphate)n
-
 ?
2.7.4.1 ADP + (phosphate)n+1
-
 Mycobacterium tuberculosis H37Rv ATP + (phosphate)n
-
 ?
2.7.4.1 ADP + (phosphate)n+1 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis H37Rv ATP + (phosphate)n
-
 ?
2.7.4.1 ADP + (phosphate)n+1
-
 Mycobacterium tuberculosis ATCC 25618 ATP + (phosphate)n
-
 ?
2.7.4.1 ADP + (phosphate)n+1 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis ATCC 25618 ATP + (phosphate)n
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 polyphosphate kinase 2
-
 Mycobacterium tuberculosis
2.7.4.1 PPK-2
-
 Mycobacterium tuberculosis
2.7.4.1 Rv3232c
-
 Mycobacterium tuberculosis
2.7.4.34 polyphosphate:GDP phosphotransferase
-
 Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
2.7.4.1 Mycobacterium tuberculosis a marginal decrease in ppk-2 transcript level is observed upon exposure of Mycobacterium tuberculosis to nutritional stress down
2.7.4.1 Mycobacterium tuberculosis ppk-2 transcripts are upregulated by 2.0-2.5 fold during mid-log, late-log and stationary phase stages of Mycobacterium tuberculosis growth in liquid cultures up

General Information

EC Number General Information Comment Organism
2.7.4.1 malfunction ppk-2 mutant strain infected guinea pigs have significantly reduced bacterial loads and tissue pathology in comparison to wild type infected guinea pigs at later stages of infection. The ppk-2 mutant strainis more tolerant to isoniazid and impaired for survival in THP-1 macrophages Mycobacterium tuberculosis
2.7.4.1 physiological function PPK-2 enzyme is required for Mycobacterium tuberculosis growth during acute and chronic stage of infection. PPK-2 enzyme contributes to the ability of Mycobacterium tuberculosis to cause disease in guinea pigs Mycobacterium tuberculosis
2.7.4.34 physiological function PPK2 contributes to Mycobacterium tuberculosis ability to cause disease in guinea pigs. PPK2 mutant-infected guinea pigs have significantly reduced bacterial loads and tissue pathology in comparison to wild-type-infected guinea pigs at later stages of infection. In comparison to the wild-type strain, the PPK2 mutant strain is more tolerant to isoniazid and impaired for survival in THP-1 macrophages Mycobacterium tuberculosis