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Literature summary extracted from

  • Esipov, R.; Abramchik, Y.; Fateev, I.; Muravyova, T.; Artemova, K.; Konstantinova, I.; Kuranova, I.; Miroshnikov, A.
    Recombinant phosphoribosyl pyrophosphate synthetases from Thermus thermophilus HB27 Isolation and properties (2016), Russ. J. Bioorg. Chem., 42, 512-521 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expression in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.011
-
 D-ribose 5-phosphate pH 8.0, 75°C Thermus thermophilus
2.7.6.1 0.013
-
 ATP pH 8.0, 75°C Thermus thermophilus
2.7.6.1 0.034
-
 D-ribose 5-phosphate pH 8.0, 75°C Thermus thermophilus
2.7.6.1 0.038
-
 ATP pH 8.0, 75°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 150000
-
 gel filtration Thermus thermophilus
2.7.6.1 158000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Thermus thermophilus Q72I58 isoform PRPPS2
-
2.7.6.1 Thermus thermophilus Q72IE8 isoform PRPPS1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Thermus thermophilus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.1 hexamer 6 * 34300, SDS-PAGE Thermus thermophilus
2.7.6.1 hexamer 6 * 34600, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.7.6.1 PRPPS1
-
 Thermus thermophilus
2.7.6.1 PRPPS2
-
 Thermus thermophilus
2.7.6.1 TT_C1184
-
 Thermus thermophilus
2.7.6.1 TT_C1274
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.6.1 0.43
-
 D-ribose 5-phosphate pH 8.0, 75°C Thermus thermophilus
2.7.6.1 0.53
-
 ATP pH 8.0, 75°C Thermus thermophilus
2.7.6.1 12
-
 ATP pH 8.0, 75°C Thermus thermophilus
2.7.6.1 13
-
 D-ribose 5-phosphate pH 8.0, 75°C Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.6.1 14
-
 ATP pH 8.0, 75°C Thermus thermophilus
2.7.6.1 39
-
 D-ribose 5-phosphate pH 8.0, 75°C Thermus thermophilus
2.7.6.1 390
-
 D-ribose 5-phosphate pH 8.0, 75°C Thermus thermophilus
2.7.6.1 960
-
 ATP pH 8.0, 75°C Thermus thermophilus