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Literature summary extracted from

  • Freedman, R.; Yu, R.; Sarkis, A.W.; Hedstrom, L.
    A structural determinant of mycophenolic acid resistance in eukaryotic inosine 5'-monophosphate dehydrogenases (2020), Protein Sci., 29, 686-694 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.205 expressed in Escherichia coli BL21(DE3) DELTAguaB cells Aspergillus nidulans
1.1.1.205 expressed in Escherichia coli BL21(DE3) DELTAguaB cells Penicillium brevicompactum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.205 A267S the mutant enzyme is inhibited by mycophenolic acid Aspergillus nidulans
1.1.1.205 S267A the mutant enzyme of isoform IMPDHA is inhibited by mycophenolic acid Penicillium brevicompactum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.205 additional information the enzyme is not inhibited by mycophenolic acid Aspergillus nidulans
1.1.1.205 additional information the enzyme is not inhibited by mycophenolic acid Penicillium brevicompactum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.205 0.015
-
 IMP wild type enzyme, at pH 8.0 and 25°C Aspergillus nidulans
1.1.1.205 0.015
-
 IMP mutant enzyme A267S, at pH 8.0 and 25°C Aspergillus nidulans
1.1.1.205 0.079
-
 IMP mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
1.1.1.205 0.093
-
 IMP wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
1.1.1.205 0.12
-
 NAD+ wild type enzyme, at pH 8.0 and 25°C Aspergillus nidulans
1.1.1.205 0.19
-
 NAD+ mutant enzyme A267S, at pH 8.0 and 25°C Aspergillus nidulans
1.1.1.205 0.33
-
 NAD+ mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
1.1.1.205 0.4
-
 NAD+ wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.205 IMP + NAD+ + H2O Aspergillus nidulans
-
 XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O Penicillium brevicompactum
-
 XMP + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.205 Aspergillus nidulans
-
-
-
1.1.1.205 Penicillium brevicompactum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.205 IMP + NAD+ + H2O
-
 Aspergillus nidulans XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O
-
 Penicillium brevicompactum XMP + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.205 IMPDH
-
 Aspergillus nidulans
1.1.1.205 IMPDHA isoform Aspergillus nidulans
1.1.1.205 IMPDHA isoform Penicillium brevicompactum
1.1.1.205 inosine 5'-monophosphate dehydrogenase
-
 Aspergillus nidulans
1.1.1.205 inosine 5'-monophosphate dehydrogenase
-
 Penicillium brevicompactum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.205 1.6
-
 NAD+ mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
1.1.1.205 1.6
-
 IMP mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
1.1.1.205 1.9
-
 NAD+ wild type enzyme, at pH 8.0 and 25°C Aspergillus nidulans
1.1.1.205 1.9
-
 IMP wild type enzyme, at pH 8.0 and 25°C Aspergillus nidulans
1.1.1.205 1.9
-
 NAD+ mutant enzyme A267S, at pH 8.0 and 25°C Aspergillus nidulans
1.1.1.205 1.9
-
 IMP mutant enzyme A267S, at pH 8.0 and 25°C Aspergillus nidulans
1.1.1.205 2.7
-
 NAD+ wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum
1.1.1.205 2.7
-
 IMP wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C Penicillium brevicompactum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.205 NAD+
-
 Aspergillus nidulans
1.1.1.205 NAD+
-
 Penicillium brevicompactum