Literature summary extracted from

Zheng, W.; Wang, Q.; Bi, Q.
Construction, expression, and characterization of recombinant Pfu DNA polymerase in Escherichia coli (2016), Protein J., 35, 145-153 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.7.7	recombinant plasmid pLysS His6-tagged Pfu-pET28a is constructed. His-tagged Pfu is expressed in Escherichia coli BL21 (DE3) competent cells	Pyrococcus furiosus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.7.7	90000	-	SDS-PAGE	Pyrococcus furiosus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.7	Pyrococcus furiosus	P61875	-	-
2.7.7.7	Pyrococcus furiosus ATCC 43587	P61875	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.7	purified with the AKTAprime plus compact one-step purification system by Ni2+ chelating affinity chromatography	Pyrococcus furiosus

Subunits

EC Number	Subunits	Comment	Organism
2.7.7.7	?	x * 90000, SDS-PAGE	Pyrococcus furiosus

Synonyms

EC Number	Synonyms	Comment	Organism
2.7.7.7	Pfu DNA polymerase	-	Pyrococcus furiosus

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
2.7.7.7	Pyrococcus furiosus	isoelectric focusing electrophoresis	7.35	6.85

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Release 2023.1 (January 2023)