Literature summary extracted from

Pantazaki, A.A.; Tambaka, M.G.; Langlois, V.; Guerin, P.; Kyriakidis, D.A.
Polyhydroxyalkanoate (PHA) biosynthesis in Thermus thermophilus purification and biochemical properties of PHA synthase (2003), Mol. Cell. Biochem., 254, 173-183 .

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.3.1.304	acetyl phosphate	10 mM acetyl phosphate activates both the native and the dephosphorylated enzyme 6fold and 2fold, respectively	Thermus thermophilus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.3.1.304	N-ethyl-maleimide	1 mM N-ethyl-maleimide for 15 min inhibits the enzyme by 56%	Thermus thermophilus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.3.1.304	cytosol	-	Thermus thermophilus	5829	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.1.304	Thermus thermophilus	-	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
2.3.1.304	phosphoprotein	-	Thermus thermophilus

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.1.304	ammonium sulfate precipitation, DEAE-cellulose column chromatography, Cibacron Blue column chromatography, omega-amino octyl-agarose column chromatography, and hydroxyapatite column chromatography	Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.3.1.304	0.48	-	crude extract, at pH 8.5 and 70°C	Thermus thermophilus
2.3.1.304	2050	-	after 4271fold purification, at pH 8.5 and 70°C	Thermus thermophilus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.1.304	DL-3-beta-hydroxybutyryl-CoA + poly[DL-3-hydroxybutyrate]n	-	Thermus thermophilus	CoA + poly[DL-3-hydroxybutyrate]n+1	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.3.1.304	?	x * 55000, SDS-PAGE	Thermus thermophilus

Synonyms

EC Number	Synonyms	Comment	Organism
2.3.1.304	PHA synthase	-	Thermus thermophilus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.3.1.304	70	-	-	Thermus thermophilus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.3.1.304	7.3	-	-	Thermus thermophilus

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Release 2023.1 (January 2023)