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Literature summary extracted from

  • Brown, A.; Sridharan, S.; Kremer, L.; Lindenberg, S.; Dover, L.; Sacchettini, J.; Besra, G.
    Probing the mechanism of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III mtFabH Factors influencing catalysis and substrate specificity (2005), J. Biol. Chem., 280, 32539-32547 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.301 expression in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.301 C122A 0.25% of wild-type activity Mycobacterium tuberculosis
2.3.1.301 H258A 0.46% of wild-type activity Mycobacterium tuberculosis
2.3.1.301 N289A 0.51% of wild-type activity Mycobacterium tuberculosis
2.3.1.301 R161A 69.7% of wild-type activity Mycobacterium tuberculosis
2.3.1.301 R46A 7.3% of wild-type activity Mycobacterium tuberculosis
2.3.1.301 R46A/R161A 0.31% of wild-type activity Mycobacterium tuberculosis
2.3.1.301 T97F 0.47% of wild-type activity Mycobacterium tuberculosis
2.3.1.301 W42A 21.5% of wild-type activity Mycobacterium tuberculosis
2.3.1.301 W42A/R161A 0.24% of wild-type activity Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.301 Mycobacterium tuberculosis P9WNG3
-
-
2.3.1.301 Mycobacterium tuberculosis H37Rv P9WNG3
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.301 0.00006
-
 substrates arachoyl-CoA and malonyl-[ACP], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.0004
-
 substrates hexanoyl-CoA and malonyl-[ACP], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.0006
-
 substrates stearoyl-CoA and malonyl-[ACP], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.0013
-
 substrates palmitoyl-CoA and malonyl-[ACP], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.00174
-
 substrates hexanoyl-CoA and malonyl-[AcpM], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.0022
-
 substrates tetradecanoyl-CoA and malonyl-[ACP], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.00549
-
 substrates octanoyl-CoA and malonyl-[AcpM], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.006
-
 substrates octanoyl-CoA and malonyl-[ACP], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.00616
-
 substrates decanoyl-CoA and malonyl-[AcpM], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.0063
-
 substrates decanoyl-CoA and malonyl-[ACP], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.00724
-
 substrates dodecanoyl-CoA and malonyl-[AcpM], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.008
-
 substrates dodecanoyl-CoA and malonyl-[ACP], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.009
-
 substrates arachoyl-CoA and malonyl-[AcpM], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.00927
-
 substrates palmitoyl-CoA and malonyl-[AcpM], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.00946
-
 substrates tetradecanoyl-CoA and malonyl-[AcpM], pH 7, 37°C Mycobacterium tuberculosis
2.3.1.301 0.0104
-
 substrates stearoyl-CoA and malonyl-[AcpM], pH 7, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.301 arachoyl-CoA + malonyl-[ACP]
-
 Mycobacterium tuberculosis 3-oxodocosanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 arachoyl-CoA + malonyl-[ACP]
-
 Mycobacterium tuberculosis H37Rv 3-oxodocosanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 decanoyl-CoA + malonyl-[ACP]
-
 Mycobacterium tuberculosis 3-oxododecanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 decanoyl-CoA + malonyl-[ACP]
-
 Mycobacterium tuberculosis H37Rv 3-oxododecanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 dodecanoyl-CoA + malonyl-[ACP]
-
 Mycobacterium tuberculosis 3-oxotetradecanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 dodecanoyl-CoA + malonyl-[ACP]
-
 Mycobacterium tuberculosis H37Rv 3-oxotetradecanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 hexanoyl-CoA + malonyl-[ACP] ACP i.e. Escherichia coli acyl-carrier protein Mycobacterium tuberculosis 3-oxooctanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 hexanoyl-CoA + malonyl-[ACP] ACP i.e. Escherichia coli acyl-carrier protein Mycobacterium tuberculosis H37Rv 3-oxooctanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 octanoyl-CoA + malonyl-[AcpM] AcpM i.e. natural partner Mycobacterium tuberculosis acyl-carrier protein Mycobacterium tuberculosis 3-oxodecanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 octanoyl-CoA + malonyl-[AcpM] AcpM i.e. natural partner Mycobacterium tuberculosis acyl-carrier protein Mycobacterium tuberculosis H37Rv 3-oxodecanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 palmitoyl-CoA + malonyl-[ACP]
-
 Mycobacterium tuberculosis 3-oxostearoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 stearoyl-CoA + malonyl-[ACP]
-
 Mycobacterium tuberculosis 3-oxoicosanoyl-[ACP] + CoA + CO2
-
 ?
2.3.1.301 tetradecanoyl-CoA + malonyl-[ACP]
-
 Mycobacterium tuberculosis 3-oxopalmitoyl-[ACP] + CoA + CO2
-
 ?