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Literature summary extracted from

  • Daubner, S.C.; Matthews, R.G.
    Purification and properties of methylenetetrahydrofolate reductase from pig liver (1982), J. Biol. Chem., 257, 140-145 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.53 0.016
-
 NADPH pH 6.7, 37°C Sus scrofa
1.5.1.53 0.019
-
 5,10-methylenetetrahydrofolate pH 6.7, 37°C Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.53 210000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.53 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.53
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.53 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.53 19.4
-
 pH 6.7, 37°C Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.53 5,10-methylenetetrahydrofolate + NADPH + H+
-
 Sus scrofa 5-methyltetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.53 ? x * 77300, SDS-PAGE, x * 74500, calcuated from sequence Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.53 26.67
-
 NADPH pH 6.7, 37°C Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.53 FAD reduction of 1 mol of enzyme-bound FAD requires 1.1 mol of NADPH. The reduced enzyme can be reoxidized by (6-R)-methylenetetrahydrofolate, again with nearly 1:1 stoichiometry Sus scrofa

General Information

EC Number General Information Comment Organism
1.5.1.53 metabolism kinetics suggest a ping-pong mechanism for methylenetetrahydrofolate reductase, or a ternary complex mechanism in which NADPH binding precedes 5,10-methylenetetrahydrofolate Sus scrofa