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Literature summary extracted from

  • Matthews, R.G.; Kaufman, S.
    Characterization of the dihydropterin reductase activity of pig liver methylenetetrahydrofolate reductase (1980), J. Biol. Chem., 255, 6014-6017 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.53 dihydropteroylhexaglutamate inhibits reduction of quinonoid dihydropterins Sus scrofa
1.5.1.53 S-adenosyl-L-methionine inhibits reduction of quinonoid dihydropterins Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.53 0.016
-
NADPH cosubstrate 5,10-methylenetetrahydrofolate, pH 7.2, 25°C Sus scrofa
1.5.1.53 0.02
-
5,10-methylenetetrahydrofolate cosubstrate NADH, pH 7.2, 25°C Sus scrofa
1.5.1.53 0.02
-
5,10-methylenetetrahydrofolate cosubstrate NADPH, pH 7.2, 25°C Sus scrofa
1.5.1.53 0.03
-
dihydrobiopterin cosubstrate NADPH, pH 7.2, 25°C Sus scrofa
1.5.1.53 0.04
-
dihydrofolate cosubstrate NADPH, pH 7.2, 25°C Sus scrofa
1.5.1.53 0.1
-
NADH cosubstrate 5,10-methylenetetrahydrofolate, pH 7.2, 25°C Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.53 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.53 liver
-
Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.53 5,10-methylenetetrahydrofolate + NADH + H+
-
Sus scrofa 5-methyltetrahydrofolate + NAD+
-
?
1.5.1.53 5,10-methylenetetrahydrofolate + NADPH + H+
-
Sus scrofa 5-methyltetrahydrofolate + NADP+
-
?
1.5.1.53 6,7-dimethyl-7,8-dihydropterin + NADH + H+
-
Sus scrofa 6,7-dimethyl-5,6,7,8-tetrahydropterin + NADP+
-
?
1.5.1.53 dihydrobiopterin + NADPH + H+
-
Sus scrofa tetrahydrobiopterin + NADP+
-
?
1.5.1.53 dihydrofolate + NADPH + H+
-
Sus scrofa tetrahydrofolate + NADP+
-
?
1.5.1.53 additional information under Vmax conditions, the turnover numbers for the NADPH-linked reductions of the quinonoid forms of 6,7-dimethyldihydropterin, dihydrobiopterin, and dihydrofolate are all about the same as that for the reduction of methylenetetrahydrofolate. The reduction of quinonoid dihydropterins is inhibited by S-adenosyl-L-methionine and dihydropteroylhexaglutamate Sus scrofa ?
-
-

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.53 NADH NADPH is 6fold preferred over NADH Sus scrofa
1.5.1.53 NADPH
-
Sus scrofa