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Literature summary extracted from

  • Gollapali, P.; Chavdi, M.; Sudharshan, S.; Hanumanthappa, M.
    Expression, purification, and characterization of pyruvate kinase from Mycobacterium tuberculosis a key allosteric regulatory enzyme (2018), Int. J. Mycobacteriol., 7, 368-374 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40 gene pyk, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Mg2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate Mycobacterium tuberculosis
-
 ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate Mycobacterium tuberculosis H37Rv
-
 ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate Mycobacterium tuberculosis ATCC 25618
-
 ATP + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Mycobacterium tuberculosis P9WKE5
-
-
2.7.1.40 Mycobacterium tuberculosis ATCC 25618 P9WKE5
-
-
2.7.1.40 Mycobacterium tuberculosis H37Rv P9WKE5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 5.31
-
 purified recombinant His-tagged enzyme, pH 7.6, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Mycobacterium tuberculosis ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate
-
 Mycobacterium tuberculosis H37Rv ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate
-
 Mycobacterium tuberculosis ATCC 25618 ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 ? x * 54000, recombinant enzyme, SDS-PAGE Mycobacterium tuberculosis
2.7.1.40 More secondary structure analysis by circular dichroism spectroscopy revealing a content of 17% alpha-helix, 34% beta-sheet, and 49% turns in the enzyme Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.40 PYK
-
 Mycobacterium tuberculosis
2.7.1.40 Rv1617
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7.6
-
 assay at Mycobacterium tuberculosis