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Literature summary extracted from

  • Vafiadi, C.; Topakas, E.; Biely, P.; Christakopoulos, P.
    Purification, characterization and mass spectrometric sequencing of a thermophilic glucuronoyl esterase from Sporotrichum thermophile (2009), FEMS Microbiol. Lett., 296, 178-184 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.117 1.3
-
 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside pH 6.0, 50°C Thermothelomyces thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.117 extracellular
-
 Thermothelomyces thermophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.117 27000
-
 gel filtration Thermothelomyces thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.117 additional information Thermothelomyces thermophilus the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols ?
-
-
3.1.1.117 additional information Thermothelomyces thermophilus ATCC 34628 the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.117 Thermothelomyces thermophilus
-
-
-
3.1.1.117 Thermothelomyces thermophilus ATCC 34628
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.117 glycoprotein StGE1 contains a noncatalytic carbohydrate-binding module Thermothelomyces thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.117 native extracellular enzyme 74fold from culture broth, by ultrafiltration, two different steps of anion exchange chromatography, again ultrafiltration, and two different steps of gel filtration, followed by hydrophobic interaction chromatography Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.117 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Thermothelomyces thermophilus methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside
-
 ?
3.1.1.117 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Thermothelomyces thermophilus ATCC 34628 methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside
-
 ?
3.1.1.117 additional information the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols Thermothelomyces thermophilus ?
-
-
3.1.1.117 additional information the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols Thermothelomyces thermophilus ATCC 34628 ?
-
-

Subunits

EC Number Subunits Comment Organism
3.1.1.117 ? x * 58000, SDS-PAGE Thermothelomyces thermophilus
3.1.1.117 More StGE1 is a modular enzyme, peptide fragmentation fingerprint, and mass spectrometric analysis, amino acid sequence comparison Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.117 glucuronoyl esterase
-
 Thermothelomyces thermophilus
3.1.1.117 StGE1
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.117 60
-
-
 Thermothelomyces thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.117 40 70 activity range, profile overview Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.117 45 55 purified esterase, 50% activity is retained after 430 and 286 min at 50°C and 55°C, and over 90% of maximal activity at 45°C after 6 h, inactivation after 6 h at 60°C Thermothelomyces thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.117 0.808
-
 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside pH 6.0, 50°C Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.117 6
-
-
 Thermothelomyces thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.117 3 9 activity range, profile overview Thermothelomyces thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.117 8
-
 purified esterase, 6 h, 50°C, over 90% activity remaining at pH 8.0, 40% activity at pH 5.0 and pH 10.0, 20% at pH 11.0, and 10% at pH 4.0, inactivation after 6 h at pH 3.0 Thermothelomyces thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.117 Thermothelomyces thermophilus isoelectric focusing
-
 6.7

General Information

EC Number General Information Comment Organism
3.1.1.117 evolution enzyme StGE1 is a family 15 glucuronoyl esterase that shows the highest homology with the hypothetical glucuronoyl esterase CHGG_10774 (UniProt ID Q2GMN0) of Chaetomium globosum strain CBS 148.51 Thermothelomyces thermophilus
3.1.1.117 physiological function the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols Thermothelomyces thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.117 0.622
-
 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside pH 6.0, 50°C Thermothelomyces thermophilus