EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.47 | biotechnology | (±)-ethyl mandelate are important intermediates in the synthesis of numerous pharmaceuticals. Efficient routes for the production of these derivatives are highly desirable. A co-immobilization strategy is developed to overcome the issue of NADPH demand in the short-chain dehydrogenase/reductase (SDR) catalytic process. The SDR from Thermus thermophilus HB8 and the NAD(P)-dependent glucose dehydrogenase (GDH) from Thermoplasma acidophilum DSM 1728 are co-immobilized on silica gel. This dual-system offers an efficient route for the biosynthesis of (+/-)-ethyl mandelate | Thermoplasma acidophilum |
1.1.1.47 | synthesis | (±)-ethyl mandelate are important intermediates in the synthesis of numerous pharmaceuticals. Efficient routes for the production of these derivatives are highly desirable. A co-immobilization strategy is developed to overcome the issue of NADPH demand in the short-chain dehydrogenase/reductase (SDR) catalytic process. The SDR from Thermus thermophilus HB8 and the NAD(P)-dependent glucose dehydrogenase (GDH) from Thermoplasma acidophilum DSM 1728 are co-immobilized on silica gel. This dual-system offers an efficient route for the biosynthesis of (+/-)-ethyl mandelate | Thermoplasma acidophilum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.47 | overexpression in Escherichia coli, short-chain dehydrogenase/reductase from Thermus thermophilus HB8 and the glucose dehydrogenase from Thermoplasma acidophilum DSM 1728 are simultaneously over-expressed by heterologous expression with pETDuet | Thermoplasma acidophilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 40250 | - |
calculated from sequence | Thermoplasma acidophilum |
EC Number | Organism | UniProt | Comment | Textmining |
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1.1.1.47 | Thermoplasma acidophilum | - |
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EC Number | Synonyms | Comment | Organism |
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1.1.1.47 | NAD(P)-dependent glucose dehydrogenase | - |
Thermoplasma acidophilum |