EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.79 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells | Sinorhizobium meliloti |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.79 | enzyme with NADP+ plus sulfate, sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M ammonium sulfate. Enzyme with NADPH plus oxalate, sitting drop vapor diffusion method, using 0.2 M ammonium citrate tribasic pH 7.0, 20% (w/v) PEG 3350. Apo enzyme form, sitting drop vapor diffusion method, using 0.1 M sodium citrate, pH 5.6, 20% (v/v) 2-propanol, 20% (w/v) PEG 4000 | Sinorhizobium meliloti |
1.1.1.81 | structures in apo forms and in complexes with cofactors and substrates/substrate analogues | Sinorhizobium meliloti |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.79 | 9.7 | - |
pyruvate | at pH 7.5 and 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 0.0105 | - |
NADPH | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 0.059 | - |
NADPH | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 5.59 | - |
phenylpyruvate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 6.14 | - |
3-hydroxypyruvate | K0.5 value, Hill coefficient 1.74, pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 6.58 | - |
phenylpyruvate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 9.35 | - |
3-hydroxypyruvate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 9.69 | - |
pyruvate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 11 | - |
2-dehydro-D-gluconate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 12.3 | - |
glyoxylate | K0.5 value, Hill coefficient 1.83, pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 80 | - |
glyoxylate | K0.5 value, Hill coefficient 1.48, pH 7.5, 25°C | Sinorhizobium meliloti |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.79 | glyoxylate + NADPH + H+ | Sinorhizobium meliloti | enzyme GhrA shows highest catalytic efficiency for glyoxylate | glycolate + NADP+ | - |
? | |
1.1.1.79 | hydroxypyruvate + NADH + H+ | Sinorhizobium meliloti | with hydroxypyruvate as a substrate at a saturating concentration (66.7 mM), the enzyme GhrA exhibits 2-3% activity with 0.4 mM NADH as compared to 0.4 mM NADPH | D-glycerate + NAD+ | - |
? | |
1.1.1.79 | hydroxypyruvate + NADPH + H+ | Sinorhizobium meliloti | - |
D-glycerate + NADP+ | - |
? | |
1.1.1.79 | additional information | Sinorhizobium meliloti | no activity with 2-oxo-D-gluconate | ? | - |
- |
|
1.1.1.79 | oxalate + NADPH + H+ | Sinorhizobium meliloti | - |
? | - |
? | |
1.1.1.79 | pyruvate + NADPH + H+ | Sinorhizobium meliloti | low efficiency | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.79 | Sinorhizobium meliloti | - |
- |
- |
1.1.1.81 | Sinorhizobium meliloti | Q92LZ4 | - |
- |
1.1.1.81 | Sinorhizobium meliloti | Q92T34 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.79 | Ni-NTA resin column chromatography and Superdex 200 pg gel filtration | Sinorhizobium meliloti |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.79 | glyoxylate + NADPH + H+ | enzyme GhrA shows highest catalytic efficiency for glyoxylate | Sinorhizobium meliloti | glycolate + NADP+ | - |
? | |
1.1.1.79 | hydroxypyruvate + NADH + H+ | with hydroxypyruvate as a substrate at a saturating concentration (66.7 mM), the enzyme GhrA exhibits 2-3% activity with 0.4 mM NADH as compared to 0.4 mM NADPH | Sinorhizobium meliloti | D-glycerate + NAD+ | - |
? | |
1.1.1.79 | hydroxypyruvate + NADPH + H+ | - |
Sinorhizobium meliloti | D-glycerate + NADP+ | - |
? | |
1.1.1.79 | additional information | no activity with 2-oxo-D-gluconate | Sinorhizobium meliloti | ? | - |
- |
|
1.1.1.79 | oxalate + NADPH + H+ | - |
Sinorhizobium meliloti | ? | - |
? | |
1.1.1.79 | pyruvate + NADPH + H+ | low efficiency | Sinorhizobium meliloti | ? | - |
? | |
1.1.1.81 | 2-dehydro-D-gluconate + NADPH + H+ | - |
Sinorhizobium meliloti | D-gluconate + NADP+ | - |
? | |
1.1.1.81 | 3-hydroxypyruvate + NADPH + H+ | - |
Sinorhizobium meliloti | D-glycerate + NADP+ | - |
? | |
1.1.1.81 | glyoxylate + NADPH + H+ | - |
Sinorhizobium meliloti | glycolate + NADP+ | - |
? | |
1.1.1.81 | phenylpyruvate + NADPH + H+ | - |
Sinorhizobium meliloti | phenyllactate + NADP+ | - |
? | |
1.1.1.81 | pyruvate + NADPH + H+ | - |
Sinorhizobium meliloti | lactate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.79 | homodimer | x-ray crystallography | Sinorhizobium meliloti |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.79 | GhrA | - |
Sinorhizobium meliloti |
1.1.1.81 | GhrA | - |
Sinorhizobium meliloti |
1.1.1.81 | GhrB | - |
Sinorhizobium meliloti |
1.1.1.81 | SMc02828 | - |
Sinorhizobium meliloti |
1.1.1.81 | SMc04462 | - |
Sinorhizobium meliloti |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.79 | NADPH | - |
Sinorhizobium meliloti | |
1.1.1.81 | NADPH | - |
Sinorhizobium meliloti |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.81 | 0.057 | - |
pyruvate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 0.27 | - |
phenylpyruvate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 0.37 | - |
phenylpyruvate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 0.81 | - |
glyoxylate | K0.5 value, Hill coefficient 1.48, pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 0.94 | - |
2-dehydro-D-gluconate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 1.2 | - |
3-hydroxypyruvate | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 1.72 | - |
glyoxylate | Hill coefficient 1.83, pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 9.31 | - |
3-hydroxypyruvate | K0.5 value, Hill coefficient 1.74, pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 371 | - |
NADPH | pH 7.5, 25°C | Sinorhizobium meliloti | |
1.1.1.81 | 773 | - |
NADPH | pH 7.5, 25°C | Sinorhizobium meliloti |