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Literature summary extracted from

  • Fernandes, P.; Kinkead, J.; McNae, I.; Bringaud, F.; Michels, P.; Walkinshaw, M.
    The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction (2019), Biochem. J., 476, 179-191 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 AMP 0.5 mM, 10fold activation of reaction with D-fructose 1,6-bisphosphate and ADP Leishmania infantum
2.7.1.11 AMP 0.5 mM, 2fold activation of reaction with D-fructose 1,6-bisphosphate and ADP Trypanosoma cruzi
2.7.1.11 AMP 0.5 mM, 2fold activation of reaction with D-fructose 1,6-bisphosphate and ADP Trypanosoma brucei brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11 expression in Escherichia coli Homo sapiens
2.7.1.11 expression in Escherichia coli Trypanosoma cruzi
2.7.1.11 expression in Escherichia coli Trypanosoma brucei brucei
2.7.1.11 expression in Escherichia coli Leishmania infantum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ADP substrate inhibition above 2 mM; substrate inhibition above 2 mM; substrate inhibition above 2 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 additional information
-
 ADP K0.5: 0.0668 mM, pH 7.4, 25°C, isoenzyme PFK-M Homo sapiens
2.7.1.11 additional information
-
 ADP K0.5: 0.3449 mM, pH 7.4, 25°C, isoenzyme PFK-L Homo sapiens
2.7.1.11 additional information
-
 D-fructose 1,6-bisphosphate K0.5: 0.7178 mM, pH 7.4, 25°C, isoenzyme PFK-P Homo sapiens
2.7.1.11 additional information
-
 D-fructose 1,6-bisphosphate K0.5: 0.804 mM, pH 7.4, 25°C, isoenzyme PFK-M Homo sapiens
2.7.1.11 additional information
-
 D-fructose 1,6-bisphosphate K0.5: 1.287 mM, pH 7.4, 25°C Trypanosoma brucei brucei
2.7.1.11 additional information
-
 ADP K0.5: 1.382 mM, pH 7.4, 25°C Trypanosoma brucei brucei
2.7.1.11 additional information
-
 D-fructose 1,6-bisphosphate K0.5: 1.54, pH 7.4, 25°C Trypanosoma cruzi
2.7.1.11 additional information
-
 ADP K0.5: 1.768 mM, pH 7.4, 25°C Trypanosoma cruzi
2.7.1.11 additional information
-
 D-fructose 1,6-bisphosphate K0.5: 2.1 mM, pH 7.4, 25°C, isoenzyme PFK-L Homo sapiens
2.7.1.11 additional information
-
 D-fructose 1,6-bisphosphate K0.5: 2.495 mM, pH 7.4, 25°C Leishmania infantum
2.7.1.11 additional information
-
 ADP K0.5: 3.137 mM, pH 7.4, 25°C Leishmania infantum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Homo sapiens P08237
-
-
2.7.1.11 Homo sapiens P17858
-
-
2.7.1.11 Homo sapiens Q01813
-
-
2.7.1.11 Leishmania infantum A4I4W5
-
-
2.7.1.11 Trypanosoma brucei brucei O15648
-
-
2.7.1.11 Trypanosoma cruzi Q4E657
-
-
2.7.1.11 Trypanosoma cruzi CL Brener Q4E657
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ADP + D-fructose 1,6-bisphosphate
-
 Trypanosoma cruzi ATP + D-fructose 6-phosphate
-
 r
2.7.1.11 ADP + D-fructose 1,6-bisphosphate
-
 Trypanosoma brucei brucei ATP + D-fructose 6-phosphate
-
 r
2.7.1.11 ADP + D-fructose 1,6-bisphosphate
-
 Leishmania infantum ATP + D-fructose 6-phosphate
-
 r
2.7.1.11 ADP + D-fructose 1,6-bisphosphate for human cells, the measured K0.5(ADP)0:5 values for the reaction with ADP and D-fructose 1,6-bisphosphate exceeds the cytosolic concentrations of ADP, but as steady-state concentrations of F0.5(D-fructose 1,6-bisphosphate) are lower than the K0.5(D-fructose 1,6-bisphosphate) values, it seems likely that under steady-state conditions the reverse reaction would indeed be inefficient. However, cells can be subjected to extreme conditions of stress and nutrient deprivation and the phosphofructokinase reverse flux may be very different in non-steady state, glucose-poor conditions and Q values of 500 or above can easily be achieved if ATP or glucose-6-phosphate are (transiently) depleted Homo sapiens ATP + D-fructose 6-phosphate
-
 r
2.7.1.11 ADP + D-fructose 1,6-bisphosphate
-
 Trypanosoma cruzi CL Brener ATP + D-fructose 6-phosphate
-
 r
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Trypanosoma cruzi ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Trypanosoma brucei brucei ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Leishmania infantum ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 ATP + D-fructose 6-phosphate all three human PFK isoforms can catalyse the reverse reaction in vitro Homo sapiens ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Trypanosoma cruzi CL Brener ADP + D-fructose 1,6-bisphosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 ATP-dependent PFK
-
 Homo sapiens
2.7.1.11 ATP-dependent PFK
-
 Trypanosoma cruzi
2.7.1.11 ATP-dependent PFK
-
 Trypanosoma brucei brucei
2.7.1.11 ATP-dependent PFK
-
 Leishmania infantum
2.7.1.11 LmPFK
-
 Leishmania infantum
2.7.1.11 PFK-L
-
 Homo sapiens
2.7.1.11 PFK-M
-
 Homo sapiens
2.7.1.11 PFK-P
-
 Homo sapiens
2.7.1.11 phosphofructokinase
-
 Homo sapiens
2.7.1.11 phosphofructokinase
-
 Trypanosoma cruzi
2.7.1.11 phosphofructokinase
-
 Trypanosoma brucei brucei
2.7.1.11 phosphofructokinase
-
 Leishmania infantum
2.7.1.11 TbPFK
-
 Trypanosoma brucei brucei
2.7.1.11 TcPFK
-
 Trypanosoma cruzi