Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Srikalaivani, R.; Singh, A.; Vijayan, M.; Surolia, A.
    Structure, interactions and action of Mycobacterium tuberculosis 3-hydroxyisobutyric acid dehydrogenase (2018), Biochem. J., 475, 2457-2471 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.31 expressed in Escherichia coli BL21(lambdaDE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.31 native enzyme, the holoenzyme, binary complexes with NAD+, (S)-3-hydroxyisobutyrate, (R)-3-hydroxyisobutyrate, L-serine and 3-hydroxypropanoic acid and ternary complexes involving the substrates and NAD+, microbatch-under-oil method, using 0.02 M MgCl2, 0.1 M HEPES (pH 7.5), 22% (w/v) polyacrylic acid sodium salt 5100 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.31 D244A the mutant shows strong reduction in the activity compared to the wild type enzyme Mycobacterium tuberculosis
1.1.1.31 D275A the mutant shows a marginal loss of activity compared to the wild type enzyme Mycobacterium tuberculosis
1.1.1.31 F236A the mutant shows reduction in the activity compared to the wild type enzyme Mycobacterium tuberculosis
1.1.1.31 K168A inactive Mycobacterium tuberculosis
1.1.1.31 N172A the mutant shows dramatic reduction in the activity compared to the wild type enzyme Mycobacterium tuberculosis
1.1.1.31 S119A inactive Mycobacterium tuberculosis
1.1.1.31 T207A the mutant shows reduction in the activity compared to the wild type enzyme Mycobacterium tuberculosis
1.1.1.31 W211A the mutant shows dramatic reduction in the activity compared to the wild type enzyme Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.31 NADH competitive inhibition in the presence of NADH when NAD+ is varied, noncompetitive inhibition with NADH when (S)-3-hydroxyisobutyrate is varied Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.31 0.563
-
 NAD+ with (S)-3-hydroxyisobutyrate as cosubstrate, pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 0.574
-
 (S)-3-hydroxyisobutyrate at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 0.582
-
 NADP+ with (S)-3-hydroxyisobutyrate as cosubstrate, pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 2.5 3 3-hydroxypropanoic acid at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 4.56
-
 (R)-3-hydroxyisobutyrate at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 21.33
-
 L-serine at pH 8.5 and 30°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.31 66000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ Mycobacterium tuberculosis the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ Mycobacterium tuberculosis H37Rv the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor 2-methyl-3-oxopropanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.31 Mycobacterium tuberculosis P9WNY5
-
-
1.1.1.31 Mycobacterium tuberculosis H37Rv P9WNY5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.31 Ni-NTA resin column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.31 (R)-3-hydroxyisobutyrate + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 (R)-3-hydroxyisobutyrate + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis H37Rv 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor Mycobacterium tuberculosis 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is specific to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor Mycobacterium tuberculosis H37Rv 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is specific to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis H37Rv 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 (S)-3-hydroxyisobutyrate + NADP+
-
 Mycobacterium tuberculosis 2-methyl-3-oxopropanoate + NADPH + H+
-
 ?
1.1.1.31 3-hydroxypropanoic acid + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis 3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 L-serine + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis ?
-
 ?
1.1.1.31 L-serine + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis H37Rv ?
-
 ?
1.1.1.31 additional information no activity with D-serine, (S)-3-hydroxyisobutyrate methyl ester, (R)-3-hydroxyisobutyrate methyl ester, L-threonine and D-threonine Mycobacterium tuberculosis ?
-
-
1.1.1.31 additional information no activity with D-serine, (S)-3-hydroxyisobutyrate methyl ester, (R)-3-hydroxyisobutyrate methyl ester, L-threonine and D-threonine Mycobacterium tuberculosis H37Rv ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.1.31 homodimer 2 * 30875, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.31 3-hydroxyisobutyric acid dehydrogenase
-
 Mycobacterium tuberculosis
1.1.1.31 HIBADH
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.31 34.5
-
 at pH 11.0, the melting temperature is 34.5°C Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.31 0.205
-
 (R)-3-hydroxyisobutyrate at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 0.262
-
 L-serine at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 0.29
-
 NADP+ with (S)-3-hydroxyisobutyrate as cosubstrate, pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 1.018
-
 (S)-3-hydroxyisobutyrate at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 1.182
-
 NAD+ with (S)-3-hydroxyisobutyrate as cosubstrate, pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 2.53
-
 3-hydroxypropanoic acid at pH 8.5 and 30°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.31 11
-
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.31 NAD+ the enzyme preferentially uses NAD+ as the cofactor Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.31 0.044
-
 L-serine at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 0.0448
-
 (R)-3-hydroxyisobutyrate at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 0.1036
-
 3-hydroxypropanoic acid at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 0.498
-
 NADP+ with (S)-3-hydroxyisobutyrate as cosubstrate, pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 1.773
-
 (S)-3-hydroxyisobutyrate at pH 8.5 and 30°C Mycobacterium tuberculosis
1.1.1.31 2.1
-
 NAD+ with (S)-3-hydroxyisobutyrate as cosubstrate, pH 8.5 and 30°C Mycobacterium tuberculosis