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Literature summary extracted from

  • Duranova, M.; Spanikova, S.; Woesten, H.A.; Biely, P.; de Vries, R.P.
    Two glucuronoyl esterases of Phanerochaete chrysosporium (2009), Arch. Microbiol., 191, 133-140 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.117 gene ge1 or e_gwh2.18.77.1, sequence analysis of the promoter region of gene ge1 reveals several putative regulatory sites, a TATA box is located -62 bp and a CCAAT-box -72 bp, upstream of ge1 translation initial site, sequence comparisons and phylogenetic analysis, functional recombinant heterologous expression of the extracellular glucuronoyl esterase in Aspergillus niger, Aspergillus vadensis strain NW282 (under control of the glucoamylase glaA promoter), Pycnoporus cinnabarinus strain BMR44 (under control of the GPD promoter), and Schizophyllum commune strain 4.8B (under control of the SC3 promoter), the enzyme expressed in Schizophyllum commune is secreted to the culture medium, the enzyme expressed in Aspergillus niger is inactive Phanerodontia chrysosporium
3.1.1.117 gene ge2 or fgenesh1_pg.C_scaffold_11000167, sequence analysis of the promoter region of gene ge2 reveals several putative regulatory sites, the ge2 promoter contains a TATA box at position -62, sequence comparisons and phylogenetic analysis, functional recombinant heterologous expression of the extracellular glucuronoyl esterase in Schizophyllum commune strain 4.8B, the enzyme is secreted to the culture medium Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.117 Phanerodontia chrysosporium P0CT87 Sporotrichum pruinosum
-
3.1.1.117 Phanerodontia chrysosporium P0CT88 Sporotrichum pruinosum
-
3.1.1.117 Phanerodontia chrysosporium ATCC MYA-4764 P0CT87 Sporotrichum pruinosum
-
3.1.1.117 Phanerodontia chrysosporium ATCC MYA-4764 P0CT88 Sporotrichum pruinosum
-
3.1.1.117 Phanerodontia chrysosporium FGSC 9002 P0CT87 Sporotrichum pruinosum
-
3.1.1.117 Phanerodontia chrysosporium FGSC 9002 P0CT88 Sporotrichum pruinosum
-
3.1.1.117 Phanerodontia chrysosporium RP-78 P0CT87 Sporotrichum pruinosum
-
3.1.1.117 Phanerodontia chrysosporium RP-78 P0CT88 Sporotrichum pruinosum
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.117 additional information
-
 recombinant enzyme expressed in Aspergillus vadensis strain NW282 shows 0.036 U/ml activity, the enzyme expressed from Pycnoporus cinnabarinus strain BMR44 shows 0.417 U/ml, and the enzyme from Schizophyllum commune strain 4.8B 0.453 U/ml, at pH 6.0, 30°C Phanerodontia chrysosporium
3.1.1.117 additional information
-
 recombinant enzyme expressed in Schizophyllum commune strain 4.8B shows 0.530 U/ml activity, at pH 6.0, 30°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.117 4-nitrophenyl-2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
3.1.1.117 4-nitrophenyl-2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Phanerodontia chrysosporium ATCC MYA-4764 ?
-
 ?
3.1.1.117 4-nitrophenyl-2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Phanerodontia chrysosporium FGSC 9002 ?
-
 ?
3.1.1.117 4-nitrophenyl-2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Phanerodontia chrysosporium RP-78 ?
-
 ?
3.1.1.117 methyl-4-O-methyl-D-glucuronate + H2O
-
 Phanerodontia chrysosporium methanol + 4-O-methyl-D-glucuronic acid
-
 ?
3.1.1.117 methyl-4-O-methyl-D-glucuronate + H2O
-
 Phanerodontia chrysosporium ATCC MYA-4764 methanol + 4-O-methyl-D-glucuronic acid
-
 ?
3.1.1.117 methyl-4-O-methyl-D-glucuronate + H2O
-
 Phanerodontia chrysosporium FGSC 9002 methanol + 4-O-methyl-D-glucuronic acid
-
 ?
3.1.1.117 methyl-4-O-methyl-D-glucuronate + H2O
-
 Phanerodontia chrysosporium RP-78 methanol + 4-O-methyl-D-glucuronic acid
-
 ?
3.1.1.117 additional information the substrate and the reaction product 4-O-methyl-D-glucuronic acid are visualized with N-(1-naphthyl)ethylenediamine dihydrochloride reagent Phanerodontia chrysosporium ?
-
-
3.1.1.117 additional information the substrate and the reaction product 4-O-methyl-D-glucuronic acid are visualized with N-(1-naphthyl)ethylenediamine dihydrochloride reagent Phanerodontia chrysosporium ATCC MYA-4764 ?
-
-
3.1.1.117 additional information the substrate and the reaction product 4-O-methyl-D-glucuronic acid are visualized with N-(1-naphthyl)ethylenediamine dihydrochloride reagent Phanerodontia chrysosporium FGSC 9002 ?
-
-
3.1.1.117 additional information the substrate and the reaction product 4-O-methyl-D-glucuronic acid are visualized with N-(1-naphthyl)ethylenediamine dihydrochloride reagent Phanerodontia chrysosporium RP-78 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
3.1.1.117 4-O-methyl-glucuronoyl methylesterase 1
-
 Phanerodontia chrysosporium
3.1.1.117 4-O-methyl-glucuronoyl methylesterase 2
-
 Phanerodontia chrysosporium
3.1.1.117 GE1
-
 Phanerodontia chrysosporium
3.1.1.117 Ge2
-
 Phanerodontia chrysosporium
3.1.1.117 glucuronoyl esterase
-
 Phanerodontia chrysosporium
3.1.1.117 glucuronoyl methylesterase 1
-
 Phanerodontia chrysosporium
3.1.1.117 glucuronoyl methylesterase 2
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.117 30
-
 assay at Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.117 6
-
 assay at Phanerodontia chrysosporium

General Information

EC Number General Information Comment Organism
3.1.1.117 evolution the amino acid sequence of GE1 is used to identify homologous genes in the genomes of twenty-four fungi. Approximately half of the genomes, both from ascomycetes and basidiomycetes, contain putative orthologues, but their presence cannot be assigned to any of fungal class or subclass. Comparison of the amino acid sequences of identified and putative glucuronoyl esterases to other types of carbohydrate esterases (CE) confirms that they form a separate family of CEs Phanerodontia chrysosporium