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Literature summary extracted from

  • Hennessy, J.E.; Latter, M.J.; Fazelinejad, S.; Philbrook, A.; Bartkus, D.M.; Kim, H.K.; Onagi, H.; Oakeshott, J.G.; Scott, C.; Alissandratos, A.; Easton, C.J.
    Hyperthermophilic carbamate kinase stability and anabolic in vitro activity at alkaline pH (2018), Appl. Environ. Microbiol., 84, e02250-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.2 expressed in Escherichia coli BL21(DE3) cells Pyrococcus furiosus
2.7.2.2 expressed in Escherichia coli BL21(DE3) cells Thermococcus sibiricus
2.7.2.2 expressed in Escherichia coli BL21(DE3) cells Thermococcus barophilus
2.7.2.2 expressed in Escherichia coli BL21(DE3) cells Fervidobacterium nodosum
2.7.2.2 expressed in Escherichia coli BL21(DE3) cells Thermosipho melanesiensis
2.7.2.2 expressed in Escherichia coli BL21(DE3) cells Enterococcus faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.2 Mg2+ 10 mM used in assay conditions Pyrococcus furiosus
2.7.2.2 Mg2+ 10 mM used in assay conditions Thermococcus sibiricus
2.7.2.2 Mg2+ 10 mM used in assay conditions Thermococcus barophilus
2.7.2.2 Mg2+ 10 mM used in assay conditions Fervidobacterium nodosum
2.7.2.2 Mg2+ 10 mM used in assay conditions Thermosipho melanesiensis
2.7.2.2 Mg2+ 10 mM used in assay conditions Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.2 ATP + carbamate Pyrococcus furiosus
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Thermococcus sibiricus
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Thermococcus barophilus
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Fervidobacterium nodosum
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Thermosipho melanesiensis
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Enterococcus faecalis
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Enterococcus faecalis ATCC 700802
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Pyrococcus furiosus ATCC 43587
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Fervidobacterium nodosum ATCC 35602
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Thermosipho melanesiensis DSM 120209
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Thermococcus sibiricus DSM 12597
-
 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate Thermococcus barophilus DSM 11836
-
 ADP + carbamoyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.2 Enterococcus faecalis P0A2X7
-
-
2.7.2.2 Enterococcus faecalis ATCC 700802 P0A2X7
-
-
2.7.2.2 Fervidobacterium nodosum A7HNY8
-
-
2.7.2.2 Fervidobacterium nodosum ATCC 35602 A7HNY8
-
-
2.7.2.2 no activity in Clostridium tetani
-
-
-
2.7.2.2 Pyrococcus furiosus P95474
-
-
2.7.2.2 Pyrococcus furiosus ATCC 43587 P95474
-
-
2.7.2.2 Thermococcus barophilus F0LK57
-
-
2.7.2.2 Thermococcus barophilus DSM 11836 F0LK57
-
-
2.7.2.2 Thermococcus sibiricus C6A5J0
-
-
2.7.2.2 Thermococcus sibiricus DSM 12597 C6A5J0
-
-
2.7.2.2 Thermosipho melanesiensis A6LPA8
-
-
2.7.2.2 Thermosipho melanesiensis DSM 120209 A6LPA8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.2 Ni-affinity column chromatography Pyrococcus furiosus
2.7.2.2 Ni-affinity column chromatography Thermococcus sibiricus
2.7.2.2 Ni-affinity column chromatography Thermococcus barophilus
2.7.2.2 Ni-affinity column chromatography Fervidobacterium nodosum
2.7.2.2 Ni-affinity column chromatography Thermosipho melanesiensis
2.7.2.2 Ni-affinity column chromatography Enterococcus faecalis

Storage Stability

EC Number Storage Stability Organism
2.7.2.2 4°C, purified enzyme, 6 months, no loss of activity Pyrococcus furiosus
2.7.2.2 4°C, purified enzyme, 6 months, no loss of activity Thermococcus barophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.2 ATP + carbamate
-
 Pyrococcus furiosus ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Thermococcus sibiricus ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Thermococcus barophilus ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Fervidobacterium nodosum ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Thermosipho melanesiensis ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Enterococcus faecalis ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Enterococcus faecalis ATCC 700802 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Pyrococcus furiosus ATCC 43587 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Fervidobacterium nodosum ATCC 35602 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Thermosipho melanesiensis DSM 120209 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Thermococcus sibiricus DSM 12597 ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + carbamate
-
 Thermococcus barophilus DSM 11836 ADP + carbamoyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.2 ? x * about 40000, SDS-PAGE Pyrococcus furiosus
2.7.2.2 ? x * about 45000, SDS-PAGE Thermococcus sibiricus
2.7.2.2 ? x * about 35000, SDS-PAGE Thermococcus barophilus
2.7.2.2 ? x * about 35000, SDS-PAGE Fervidobacterium nodosum
2.7.2.2 ? x * about 35000, SDS-PAGE Thermosipho melanesiensis
2.7.2.2 ? x * about 35000, SDS-PAGE Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.2 40
-
-
 Fervidobacterium nodosum
2.7.2.2 80
-
-
 Pyrococcus furiosus
2.7.2.2 80
-
-
 Thermococcus sibiricus
2.7.2.2 80
-
-
 Thermococcus barophilus
2.7.2.2 80
-
-
 Thermosipho melanesiensis
2.7.2.2 80
-
-
 Enterococcus faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.2 40
-
 the enzyme is inactivated after incubation at 40°C for 60 h Fervidobacterium nodosum
2.7.2.2 40
-
 there is no measurable change in catalysis after incubation at 40°C for 60 h Pyrococcus furiosus
2.7.2.2 40
-
 there is no measurable change in catalysis after incubation at 40°C for 60 h Thermococcus sibiricus
2.7.2.2 40
-
 there is no measurable change in catalysis after incubation at 40°C for 60 h Thermococcus barophilus
2.7.2.2 40
-
 there is no measurable change in catalysis after incubation at 40°C for 60 h Thermosipho melanesiensis
2.7.2.2 40
-
 there is no measurable change in catalysis after incubation at 40°C for 60 h Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.2 9.9
-
-
 Fervidobacterium nodosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.2 7.4 11.4 the enzyme is active over the entire pH range studied (7.4-11.4), with optimum activity at pH 9.9 and above Pyrococcus furiosus
2.7.2.2 9.4 11.4 the enzyme shows high activity between pH 9.4 and 11.4 Thermococcus sibiricus
2.7.2.2 9.4 11.4 the enzyme shows high activity between pH 9.4 and 11.4 Thermococcus barophilus