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Literature summary extracted from

  • Lee, H.S.; Park, J.; Yoo, Y.J.; Yeon, Y.J.
    Engineering D-lactate dehydrogenase from Pediococcus acidilactici for improved ctivity on 2-hydroxy acids with bulky C3 functional group (2019), Appl. Biochem. Biotechnol., 189, 1141-1155 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.28 homology modeling of structure Pediococcus acidilactici

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.28 F298L negligible activity Pediococcus acidilactici
1.1.1.28 F298V negligible activity Pediococcus acidilactici
1.1.1.28 N76A reduced activity compared to wild-type Pediococcus acidilactici
1.1.1.28 N76I negligible activity Pediococcus acidilactici
1.1.1.28 N76L reduced activity compared to wild-type Pediococcus acidilactici
1.1.1.28 N76V reduced activity compared to wild-type Pediococcus acidilactici
1.1.1.28 V77I reduced activity compared to wild-type Pediococcus acidilactici
1.1.1.28 Y100F reduced activity compared to wild-type Pediococcus acidilactici
1.1.1.28 Y100I negligible activity Pediococcus acidilactici
1.1.1.28 Y100L negligible activity Pediococcus acidilactici
1.1.1.28 Y51A mutant with improved catalytic efficiency on phenylpyruvate Pediococcus acidilactici
1.1.1.28 Y51F mutant with improved catalytic efficiency on 2-oxobutanoate and 3-methyl-2-oxobutanoate of 37.2 and 23.2 per s and mM, respectively Pediococcus acidilactici
1.1.1.28 Y51L mutant with improved catalytic efficiency on phenylpyruvate of 2200 per s and mM Pediococcus acidilactici
1.1.1.28 Y51M mutant with improved catalytic efficiency on phenylpyruvate Pediococcus acidilactici
1.1.1.28 Y51S mutant with improved catalytic efficiency on phenylpyruvate Pediococcus acidilactici

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 0.16
-
 phenylpyruvate mutant Y51A, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 0.17
-
 phenylpyruvate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 0.22
-
 phenylpyruvate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 0.22
-
 phenylpyruvate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 0.4
-
 phenylpyruvate mutant Y51S, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 2.6
-
 phenylpyruvate wild-type, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 2.7
-
 2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 7.5
-
 2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 7.7
-
 3-methyl-2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 8.6
-
 3-methyl-2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 11
-
 2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 12
-
 2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 12
-
 3-methyl-2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 38
-
 3-methyl-2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Pediococcus acidilactici E0NDE9
-
-
1.1.1.28 Pediococcus acidilactici DSM 20284 E0NDE9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 2-oxobutanoate + NADH + H+
-
 Pediococcus acidilactici 2-hydroxybutanoate + NAD+
-
 ?
1.1.1.28 2-oxobutanoate + NADH + H+
-
 Pediococcus acidilactici DSM 20284 2-hydroxybutanoate + NAD+
-
 ?
1.1.1.28 3-methyl-2-oxobutanoate + NADH + H+
-
 Pediococcus acidilactici 2-hydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.28 3-methyl-2-oxobutanoate + NADH + H+
-
 Pediococcus acidilactici DSM 20284 2-hydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.28 phenylpyruvate + NADH + H+
-
 Pediococcus acidilactici (R)-3-phenyllactate + NAD+
-
 ?
1.1.1.28 phenylpyruvate + NADH + H+
-
 Pediococcus acidilactici DSM 20284 (R)-3-phenyllactate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 D-LDH
-
 Pediococcus acidilactici
1.1.1.28 ldhd
-
 Pediococcus acidilactici

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.28 33.9
-
 3-methyl-2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 41.3
-
 phenylpyruvate wild-type, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 50.7
-
 2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 76.2
-
 3-methyl-2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 90.2
-
 3-methyl-2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 99.4
-
 2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 152
-
 2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 179
-
 3-methyl-2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 199
-
 2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 239
-
 phenylpyruvate mutant Y51S, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 269
-
 phenylpyruvate mutant Y51A, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 376
-
 phenylpyruvate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 391
-
 phenylpyruvate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 462
-
 phenylpyruvate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.28 0.89
-
 3-methyl-2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 4.4
-
 2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 6 12 phenylpyruvate mutant Y51S, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 7.4
-
 3-methyl-2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 8.9
-
 3-methyl-2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 15.9
-
 phenylpyruvate wild-type, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 16.8
-
 2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 20.7
-
 2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 23.2
-
 3-methyl-2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 37.2
-
 2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 1720
-
 phenylpyruvate mutant Y51A, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 1880
-
 phenylpyruvate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 2130
-
 phenylpyruvate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
1.1.1.28 2200
-
 phenylpyruvate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici