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Literature summary extracted from

  • Araya-Cloutier, C.; Martens, B.; Schaftenaar, G.; Leipoldt, F.; Gruppen, H.; Vincken, J.
    Structural basis for non-genuine phenolic acceptor substrate specificity of Streptomyces roseochromogenes prenyltransferase CloQ from the ABBA/PT-barrel superfamily (2017), PLoS ONE, 12, e0174665 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.111 expressed in Escherichia coli Rosetta (DE3) pLysS cells Streptomyces roseochromogenus subsp. oscitans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.111 Mg2+ 7 mM used in assay conditions Streptomyces roseochromogenus subsp. oscitans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate Streptomyces roseochromogenus subsp. oscitans
-
 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.111 Streptomyces roseochromogenus subsp. oscitans Q8GHB2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.111 HisTrap column chromatography Streptomyces roseochromogenus subsp. oscitans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.111 dimethylallyl diphosphate + 3'-hydroxydaidzein 2% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4'-hydroxy-6-methoxyflavone 1% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4'-hydroxy-7-methoxyflavone 2% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4'-hydroxy-7-methoxyflavone 2% conversion Streptomyces roseochromogenus subsp. oscitans diphosphate + 2-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-7-methoxy-4H-chromen-4-one + 7-methoxy-2-[4-[(3-methylbut-2-en-1-yl)oxy]phenyl]-4H-chromen-4-one
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
-
 Streptomyces roseochromogenus subsp. oscitans diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate 84% conversion Streptomyces roseochromogenus subsp. oscitans diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 7,4'-dihydroxyflavone 4% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 dimethylallyl diphosphate + coumestrol 1% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 dimethylallyl diphosphate + daidzein less than 1% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 dimethylallyl diphosphate + equol 8% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 dimethylallyl diphosphate + equol 2% conversion Streptomyces roseochromogenus subsp. oscitans diphosphate + 3-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-3,4-dihydro-2H-chromen-7-ol + 3-(4-hydroxyphenyl)-6-(3-methylbut-2-en-1-yl)-3,4-dihydro-2H-chromen-7-ol
-
 ?
2.5.1.111 dimethylallyl diphosphate + genistein less than 1% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 dimethylallyl diphosphate + luteolin 3% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 dimethylallyl diphosphate + resveratrol 2% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
2.5.1.111 additional information the enzyme shows no activity with isoflavone and glycitein Streptomyces roseochromogenus subsp. oscitans ?
-
-

Subunits

EC Number Subunits Comment Organism
2.5.1.111 ? x * 35000, SDS-PAGE Streptomyces roseochromogenus subsp. oscitans

Synonyms

EC Number Synonyms Comment Organism
2.5.1.111 CloQ
-
 Streptomyces roseochromogenus subsp. oscitans