EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.21 | structures of Lin1840 wild-type and mutant D270A in complexes with sophorose and laminaribiose. Residue Arg572 forms many hydrogen bonds with sophorose and laminaribiose at subsite +1, a key factor for substrate selectivity. The opposite side of subsite +1 from Arg572 is connected to a large empty space, which may be subsite +2 for the binding of sophorotriose | Listeria innocua Clip11262 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.21 | D270A | nucleophile mutant, crystallization data | Listeria innocua Clip11262 |
3.2.1.21 | R572K | mutation in residue important for substrate recognition, 10fold increase in Km value for sophorose | Listeria innocua Clip11262 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | 1-deoxynojirimycin | competitive | Listeria innocua Clip11262 | |
3.2.1.21 | cellobiose | mixed type inhibition | Listeria innocua Clip11262 | |
3.2.1.21 | D-glucose | mixed type inhibition | Listeria innocua Clip11262 | |
3.2.1.21 | gentiobiose | mixed type inhibition | Listeria innocua Clip11262 | |
3.2.1.21 | gluconic acid delta-lactone | competitive | Listeria innocua Clip11262 | |
3.2.1.21 | isofagomine D-tartrate | competitive | Listeria innocua Clip11262 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 2 | - |
sophorose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 2.7 | - |
laminaribiose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 3.1 | - |
4-nitrophenyl-beta-D-glucoside | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 3.7 | - |
laminaritriose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 5.4 | - |
laminaritetraose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 5.5 | - |
Laminaripentaose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 9.2 | - |
laminaribiose | mutant R572K, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 10 | - |
sophorotetraose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 11 | - |
sophoropentaose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 15 | - |
sophorotriose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 21 | - |
sophorose | mutant R572K, pH 6.0, 20°C | Listeria innocua Clip11262 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 150000 | - |
gel filtration | Listeria innocua Clip11262 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Listeria innocua Clip11262 | Q92AS9 | - |
- |
3.2.1.21 | Listeria innocua Clip11262 ATCC BAA-680 | Q92AS9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 4-nitrophenyl-beta-D-glucoside + H2O | - |
Listeria innocua Clip11262 | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.21 | 4-nitrophenyl-beta-D-glucoside + H2O | - |
Listeria innocua Clip11262 ATCC BAA-680 | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.21 | laminaribiose + H2O | - |
Listeria innocua Clip11262 | 2 D-glucose | - |
? | |
3.2.1.21 | laminaribiose + H2O | - |
Listeria innocua Clip11262 ATCC BAA-680 | 2 D-glucose | - |
? | |
3.2.1.21 | laminaripentaose + H2O | - |
Listeria innocua Clip11262 | laminaritetraose + D-glucose | - |
? | |
3.2.1.21 | laminaritetraose + H2O | - |
Listeria innocua Clip11262 | laminaritriose + D-glucose | - |
? | |
3.2.1.21 | laminaritriose + H2O | - |
Listeria innocua Clip11262 | laminaribiose + D-glucose | - |
? | |
3.2.1.21 | laminaritriose + H2O | - |
Listeria innocua Clip11262 ATCC BAA-680 | laminaribiose + D-glucose | - |
? | |
3.2.1.21 | additional information | no substrates: cellobiose, gentiobiose | Listeria innocua Clip11262 | ? | - |
- |
|
3.2.1.21 | additional information | no substrates: cellobiose, gentiobiose | Listeria innocua Clip11262 ATCC BAA-680 | ? | - |
- |
|
3.2.1.21 | sophoropentaose + H2O | - |
Listeria innocua Clip11262 | sophorotetraose + D-glucose | - |
? | |
3.2.1.21 | sophorose + H2O | - |
Listeria innocua Clip11262 | 2 D-glucose | - |
? | |
3.2.1.21 | sophorose + H2O | - |
Listeria innocua Clip11262 ATCC BAA-680 | 2 D-glucose | - |
? | |
3.2.1.21 | sophorotetraose + H2O | - |
Listeria innocua Clip11262 | sophorotriose + D-glucose | - |
? | |
3.2.1.21 | sophorotriose + H2O | - |
Listeria innocua Clip11262 | sophorose + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | dimer | 2 * 79466, calculated from sequence | Listeria innocua Clip11262 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.21 | Lin1840 | - |
Listeria innocua Clip11262 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 37 | - |
- |
Listeria innocua Clip11262 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 40 | - |
drastic loss of activity above | Listeria innocua Clip11262 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 1 | - |
sophoropentaose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 3.5 | - |
sophorotetraose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 11 | - |
laminaritriose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 12 | - |
Laminaripentaose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 14 | - |
laminaribiose | mutant R572K, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 14 | - |
laminaritetraose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 15 | - |
sophorose | mutant R572K, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 23 | - |
laminaribiose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 41 | - |
sophorose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 48 | - |
4-nitrophenyl-beta-D-glucoside | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 56 | - |
sophorotriose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 6 | - |
- |
Listeria innocua Clip11262 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5 | 9 | - |
Listeria innocua Clip11262 |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.0041 | - |
isofagomine D-tartrate | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 0.29 | - |
1-deoxynojirimycin | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 0.3 | - |
gluconic acid delta-lactone | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 2.8 | - |
gentiobiose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 3.8 | - |
D-glucose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 21 | - |
cellobiose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.098 | - |
sophoropentaose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 0.34 | - |
sophorotetraose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 0.69 | - |
sophorose | mutant R572K, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 1.5 | - |
laminaribiose | mutant R572K, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 2.1 | - |
Laminaripentaose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 2.6 | - |
laminaritetraose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 2.9 | - |
laminaritriose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 3.8 | - |
sophorotriose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 8.8 | - |
laminaribiose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 15 | - |
4-nitrophenyl-beta-D-glucoside | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 | |
3.2.1.21 | 21 | - |
sophorose | wild-type, pH 6.0, 20°C | Listeria innocua Clip11262 |