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Literature summary extracted from

  • Igamberdiev, A.; Lernmark, U.; Gardestroem, P.
    Activity of the mitochondrial pyruvate dehydrogenase complex in plants is stimulated in the presence of malate (2014), Mitochondrion, 19, 184-190 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.104 malate malate is stimulatory for the mitochondrial pyruvate dehydrogenase complex, but not for barley chloroplast pyruvate dehydrogenase complex. The stimulation is saturated below 1 mM malate and is related to the partially activated complex, which activity increases in the presence of malate by about twofold. Malate also reverses the reduction of pyruvate dehydrogenase complex activity in the presence of glycine Hordeum vulgare
1.2.1.104 malate malate is stimulatory for the mitochondrial pyruvate dehydrogenase complex, but not for barley chloroplast pyruvate dehydrogenase complex. The stimulation is saturated below 1 mM malate and is related to the partially activated complex, which activity increases in the presence of malate by about twofold. Malate also reverses the reduction of pyruvate dehydrogenase complex activity in the presence of glycine Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.104 0.0132
-
 CoA absence of malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
1.2.1.104 0.076
-
 NAD+ absence of malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
1.2.1.104 0.123
-
 pyruvate absence of malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
1.2.1.104 0.128
-
 pyruvate presence of 1 mM malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
1.2.1.104 0.136
-
 NAD+ presence of 1 mM malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
1.2.1.104 0.61
-
 CoA presence of 1 mM malate, pH 7.6, temperature not specified in the publication Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.104 mitochondrion
-
 Hordeum vulgare 5739
-
1.2.1.104 mitochondrion
-
 Pisum sativum 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Hordeum vulgare
-
-
-
1.2.1.104 Pisum sativum P52904 and P52902 and P31023 P52904 i.e. E1 component subunit beta, cf. EC 1.2.4.1, P52902 i.e. E1 component subunit alpha, cf. EC 1.2.4.1, P31023 i.e. dihydrolipoyl dehydrogenase, cf. EC 1.8.1.4
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.104 leaf
-
 Hordeum vulgare
-
1.2.1.104 leaf
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.104 pyruvate + CoA + NAD+
-
 Hordeum vulgare acetyl-CoA + CO2 + NADH
-
 ?