Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shimosaka, T.; Makarova, K.S.; Koonin, E.V.; Atomi, H.
    Identification of dephospho-coenzyme A (dephospho-CoA) kinase in Thermococcus kodakarensis and elucidation of the entire CoA biosynthesis pathway in Archaea (2019), mBio, 10, e01146-19 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.24 gene TK1697, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli Thermococcus kodakarensis
2.7.1.237 gene TK1697, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.24 additional information disruption of the TK1697 resulting in CoA auxotrophy. The DELTATK1697 mutant is inoculated in ASW-YT-pyruvate-agmatine medium, but no growth is observed. When 1 mM CoA is added to the medium, the growth defect is partially complemented, with lower growth rate and less cell yield than the wild-type strain KPD1. In the strain transformed with pRPETK1697, the growth defect is almost fully complemented Thermococcus kodakarensis
2.7.1.237 additional information disruption of the TK1697 resulting in CoA auxotrophy. The DELTATK1697 mutant is inoculated in ASW-YT-pyruvate-agmatine medium, but no growth is observed. When 1 mM CoA is added to the medium, the growth defect is partially complemented, with lower growth rate and less cell yield than the wild-type strain KPD1. In the strain transformed with pRPETK1697, the growth defect is almost fully complemented Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.24 additional information excess concentrations of CoA inhibit growth Thermococcus kodakarensis
2.7.1.24 UTP at high concentrations Thermococcus kodakarensis
2.7.1.237 additional information excess concentrations of CoA inhibit growth Thermococcus kodakarensis
2.7.1.237 UTP at high concentrations Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.24 additional information
-
 additional information Michaelis-Menten kinetics Thermococcus kodakarensis
2.7.1.24 0.14
-
 3'-dephospho-CoA pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
2.7.1.24 0.26
-
 GTP pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
2.7.1.237 additional information
-
 additional information Michaelis-Menten kinetics Thermococcus kodakarensis
2.7.1.237 0.14
-
 3'-dephospho-CoA pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
2.7.1.237 0.26
-
 GTP pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.24 20800
-
 recombinant enzyme, gel filtration Thermococcus kodakarensis
2.7.1.237 20800
-
 recombinant enzyme, gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.24 Thermococcus kodakarensis Q5JIY7
-
-
2.7.1.24 Thermococcus kodakarensis ATCC BAA-918 Q5JIY7
-
-
2.7.1.24 Thermococcus kodakarensis JCM 12380 Q5JIY7
-
-
2.7.1.237 Thermococcus kodakarensis Q5JIY7
-
-
2.7.1.237 Thermococcus kodakarensis ATCC BAA-918 Q5JIY7
-
-
2.7.1.237 Thermococcus kodakarensis JCM 12380 Q5JIY7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.24 recombinant enzyme from Escherichia coli to homogeneity Thermococcus kodakarensis
2.7.1.237 recombinant enzyme from Escherichia coli to homogeneity Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.24 ATP + 3'-dephospho-CoA low activity Thermococcus kodakarensis ADP + CoA
-
 ?
2.7.1.24 ATP + 3'-dephospho-CoA low activity Thermococcus kodakarensis JCM 12380 ADP + CoA
-
 ?
2.7.1.24 ATP + 3'-dephospho-CoA low activity Thermococcus kodakarensis ATCC BAA-918 ADP + CoA
-
 ?
2.7.1.24 GTP + 3'-dephospho-CoA gene TK1697 encodes a GTP-dependent dephospho-CoA kinase Thermococcus kodakarensis GDP + CoA
-
 ?
2.7.1.24 GTP + 3'-dephospho-CoA gene TK1697 encodes a GTP-dependent dephospho-CoA kinase Thermococcus kodakarensis JCM 12380 GDP + CoA
-
 ?
2.7.1.24 GTP + 3'-dephospho-CoA gene TK1697 encodes a GTP-dependent dephospho-CoA kinase Thermococcus kodakarensis ATCC BAA-918 GDP + CoA
-
 ?
2.7.1.24 additional information when NADx02, ADP, AMP, adenosine, or ribose are substituted for dephospho-CoA as phosphate acceptors, no detectable amount of GDP is produced, indicating specificity of the TK1697 protein toward dephospho-CoA. GTP is the preferred phosphate donor Thermococcus kodakarensis ?
-
-
2.7.1.24 additional information when NADx02, ADP, AMP, adenosine, or ribose are substituted for dephospho-CoA as phosphate acceptors, no detectable amount of GDP is produced, indicating specificity of the TK1697 protein toward dephospho-CoA. GTP is the preferred phosphate donor Thermococcus kodakarensis JCM 12380 ?
-
-
2.7.1.24 additional information when NADx02, ADP, AMP, adenosine, or ribose are substituted for dephospho-CoA as phosphate acceptors, no detectable amount of GDP is produced, indicating specificity of the TK1697 protein toward dephospho-CoA. GTP is the preferred phosphate donor Thermococcus kodakarensis ATCC BAA-918 ?
-
-
2.7.1.237 ATP + 3'-dephospho-CoA low activity Thermococcus kodakarensis ADP + CoA
-
 ?
2.7.1.237 ATP + 3'-dephospho-CoA low activity Thermococcus kodakarensis JCM 12380 ADP + CoA
-
 ?
2.7.1.237 ATP + 3'-dephospho-CoA low activity Thermococcus kodakarensis ATCC BAA-918 ADP + CoA
-
 ?
2.7.1.237 GTP + 3'-dephospho-CoA gene TK1697 encodes a GTP-dependent dephospho-CoA kinase Thermococcus kodakarensis GDP + CoA
-
 ?
2.7.1.237 additional information when NADx02, ADP, AMP, adenosine, or ribose are substituted for dephospho-CoA as phosphate acceptors, no detectable amount of GDP is produced, indicating specificity of the TK1697 protein toward dephospho-CoA. GTP is the preferred phosphate donor Thermococcus kodakarensis ?
-
-
2.7.1.237 additional information when NADx02, ADP, AMP, adenosine, or ribose are substituted for dephospho-CoA as phosphate acceptors, no detectable amount of GDP is produced, indicating specificity of the TK1697 protein toward dephospho-CoA. GTP is the preferred phosphate donor Thermococcus kodakarensis JCM 12380 ?
-
-
2.7.1.237 additional information when NADx02, ADP, AMP, adenosine, or ribose are substituted for dephospho-CoA as phosphate acceptors, no detectable amount of GDP is produced, indicating specificity of the TK1697 protein toward dephospho-CoA. GTP is the preferred phosphate donor Thermococcus kodakarensis ATCC BAA-918 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.1.24 monomer 1 * 19657, sequence calculation Thermococcus kodakarensis
2.7.1.237 monomer 1 * 19657, sequence calculation Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.24 dephospho-CoA kinase
-
 Thermococcus kodakarensis
2.7.1.24 dephospho-coenzyme A kinase
-
 Thermococcus kodakarensis
2.7.1.24 DPCK
-
 Thermococcus kodakarensis
2.7.1.24 GTP-dependent dephospho-CoA kinase UniProt Thermococcus kodakarensis
2.7.1.24 GTP-dependent DPCK
-
 Thermococcus kodakarensis
2.7.1.24 TK1697
-
 Thermococcus kodakarensis
2.7.1.237 dephospho-CoA kinase
-
 Thermococcus kodakarensis
2.7.1.237 dephospho-coenzyme A kinase
-
 Thermococcus kodakarensis
2.7.1.237 DPCK
-
 Thermococcus kodakarensis
2.7.1.237 GTP-dependent dephospho-CoA kinase UniProt Thermococcus kodakarensis
2.7.1.237 GTP-dependent DPCK
-
 Thermococcus kodakarensis
2.7.1.237 TK1697
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.24 80
-
 recombinant enzyme Thermococcus kodakarensis
2.7.1.237 80
-
 recombinant enzyme Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.24 70 90 over 50% of maximal activity within this range Thermococcus kodakarensis
2.7.1.237 70 90 over 50% of maximal activity within this range Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.24 70 80 purified recombinant enzyme, pH 8.0, 2 h, no loss of activity Thermococcus kodakarensis
2.7.1.24 90
-
 purified recombinant enzyme, pH 8.0, half-life at 90°C is 24 min Thermococcus kodakarensis
2.7.1.237 70 80 purified recombinant enzyme, pH 8.0, 2 h, no loss of activity Thermococcus kodakarensis
2.7.1.237 90
-
 purified recombinant enzyme, pH 8.0, half-life at 90°C is 24 min Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.24 5.57
-
 3'-dephospho-CoA pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
2.7.1.24 6.68
-
 GTP pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
2.7.1.237 5.57
-
 3'-dephospho-CoA pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
2.7.1.237 6.68
-
 GTP pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.24 8
-
 recombinant enzyme Thermococcus kodakarensis
2.7.1.237 8
-
 recombinant enzyme Thermococcus kodakarensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.24 7 9 over 60% of maximal activity within this range Thermococcus kodakarensis
2.7.1.237 7 9 over 60% of maximal activity within this range Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
2.7.1.24 evolution enzyme DPCK, encoded by the TK1697 gene of Thermococcus kodakarensis is not homologous to the classical DPCK from bacteria and eukaryotes but is distantly related to bacterial and eukaryotic thiamine diphosphokinases. Orthologues of TK1697 are widely distributed in archaea, suggesting that this form of DPCK is responsible for the last step of CoA biosynthesis in most of the archaea Thermococcus kodakarensis
2.7.1.24 malfunction disruption of the TK1697 results in CoA auxotrophy Thermococcus kodakarensis
2.7.1.24 metabolism the pathway for CoA biosynthesis in archaea compared to those of plants and bacteria, overview Thermococcus kodakarensis
2.7.1.24 physiological function TK1697 gene-encoded DPCK contributes to CoA biosynthesis in Thermococcus kodakarensis. CoA is utilized in a wide range of metabolic pathways, and its biosynthesis is essential for life Thermococcus kodakarensis
2.7.1.237 evolution enzyme DPCK, encoded by the TK1697 gene of Thermococcus kodakarensis is not homologous to the classical DPCK from bacteria and eukaryotes but is distantly related to bacterial and eukaryotic thiamine diphosphokinases. Orthologues of TK1697 are widely distributed in archaea, suggesting that this form of DPCK is responsible for the last step of CoA biosynthesis in most of the archaea Thermococcus kodakarensis
2.7.1.237 malfunction disruption of the TK1697 results in CoA auxotrophy Thermococcus kodakarensis
2.7.1.237 metabolism the pathway for CoA biosynthesis in archaea compared to those of plants and bacteria, overview Thermococcus kodakarensis
2.7.1.237 physiological function TK1697 gene-encoded DPCK contributes to CoA biosynthesis in Thermococcus kodakarensis. CoA is utilized in a wide range of metabolic pathways, and its biosynthesis is essential for life Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.24 25.69
-
 GTP pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
2.7.1.24 39.79
-
 3'-dephospho-CoA pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
2.7.1.237 25.69
-
 GTP pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
2.7.1.237 39.79
-
 3'-dephospho-CoA pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis