Any feedback?
Literature summary extracted from
- Cloning, purification and characterization of a novel recombinant trehalose synthase (TreS) from Acidiplasma sp. MBA-1 (2019), J. Microbiol. Biotechnol. Food Sci., 8, 1298-1302 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.16 | EDTA | 1 mM, slightly enhances the activity (9.6%) | Acidiplasma sp. MBA-1 |  |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | synthesis | the enzyme (TreS) produced from Acidiplasma spMBA-1 can catalyze a considerable amount of maltose into trehalose in a single step reaction. Maltose is a relatively cheap substrate. Hence, TreS can be used as an alternative commercial enzyme to produce trehalose commercially. A significant amount of glucose is being produced as a byproduct which hinders the production of commercial trehalose. If it is possible to suppress glucose production by genetic modifications, it could enhance trehalose production | Acidiplasma sp. MBA-1 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | expression in Escherichia coli BL21 (DE) | Acidiplasma sp. MBA-1 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.16 | Co2+ | 1 mM, 38% loss of activity | Acidiplasma sp. MBA-1 | |
| 5.4.99.16 | Cu2+ | 1 mM, complete inhibition | Acidiplasma sp. MBA-1 | |
| 5.4.99.16 | Ni2+ | 1 mM, 58% loss of activity | Acidiplasma sp. MBA-1 | |
| 5.4.99.16 | Zn2+ | 1 mM, 67% loss of activity | Acidiplasma sp. MBA-1 | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.16 | Li+ | 1 mM, slightly enhances the activity (6.7%) | Acidiplasma sp. MBA-1 | |
| 5.4.99.16 | Mg2+ | 1 mM, slightly enhances the activity (10.9%) | Acidiplasma sp. MBA-1 | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 65900 | - |
SDS-PAGE | Acidiplasma sp. MBA-1 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.16 | Acidiplasma sp. MBA-1 | A0A0D8DNG2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | - |
Acidiplasma sp. MBA-1 |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 3.57 | - |
pH and temperature not specified in the publication | Acidiplasma sp. MBA-1 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | alpha,alpha-trehalose | - |
Acidiplasma sp. MBA-1 | maltose | - |
? | |
| 5.4.99.16 | maltose | conversion of trehalose from maltose is 43.62%. At the same time, TreS produces about 23.85% glucose as a by product after 10 h of incubation | Acidiplasma sp. MBA-1 | alpha,alpha-trehalose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | ? | x * 65000, SDS-PAGE | Acidiplasma sp. MBA-1 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | TreS | - |
Acidiplasma sp. MBA-1 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 40 | - |
recombinant enzyme | Acidiplasma sp. MBA-1 |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 25 | 55 | 25°C: about 40% of maximal activity, 55°C: about 60% of maximal activity, recombinant enzyme | Acidiplasma sp. MBA-1 |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 40 | - |
1 h, stable | Acidiplasma sp. MBA-1 |
| 5.4.99.16 | 45 | - |
1 h, about 10% loss of activity | Acidiplasma sp. MBA-1 |
| 5.4.99.16 | 50 | - |
1 h, about 30% loss of activity | Acidiplasma sp. MBA-1 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 6.5 | - |
recombinant enzyme | Acidiplasma sp. MBA-1 |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 5 | 7.5 | pH 5.0: about 80% of maximal activity, pH 7.5: about 50% of maximal activity | Acidiplasma sp. MBA-1 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
