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Literature summary extracted from

  • Garcia-Huertas, P.; Mejia-Jaramillo, A.M.; Gonzalez, L.; Triana-Chavez, O.
    Transcriptome and functional genomics reveal the participation of adenine phosphoribosyltransferase in Trypanosoma cruzi resistance to benznidazole (2017), J. Cell. Biochem., 118, 1936-1945 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.7 transcriptome sequencing and analysis, recombinant overexpression of GFP-tagged enzyme in Trypanosoma cruzi parasites, subcloning in Escherichia coli strain DH5alpha, quantitative RT-PCR expression analysis Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.7 benznidazole transcriptome and functional genomics reveal the participation of adenine phosphoribosyltransferase in Trypanosoma cruzi resistance to benznidazole Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Trypanosoma cruzi
-
 AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Trypanosoma cruzi MHOM
-
 AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Trypanosoma cruzi DA (TcI)
-
 AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Trypanosoma cruzi COL
-
 AMP + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Trypanosoma cruzi
-
-
-
2.4.2.7 Trypanosoma cruzi COL
-
-
-
2.4.2.7 Trypanosoma cruzi DA (TcI)
-
-
-
2.4.2.7 Trypanosoma cruzi MHOM
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.7 epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Trypanosoma cruzi AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Trypanosoma cruzi MHOM AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Trypanosoma cruzi DA (TcI) AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Trypanosoma cruzi COL AMP + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.7 ? x * 25860, recombinant enzyme, SDS-PAGE Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
2.4.2.7 APRT
-
 Trypanosoma cruzi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.2.7 Trypanosoma cruzi recombinant enzyme, isoelectric focusing
-
 7.32

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.2.7 0.0327
-
 DA strain, pH and temperature not specified in the publication Trypanosoma cruzi benznidazole

Expression

EC Number Organism Comment Expression
2.4.2.7 Trypanosoma cruzi the gene encoding adenine phosphoribosyltransferase (APRT) is downregulated by 5.2fold in the parasites resistant against benznidazole (Bz) and nifurtimox (Nfx), and its expression profile is confirmed by 2D electrophoresis analysis down
2.4.2.7 Trypanosoma cruzi overexpression of APRT (pTREX-APRT) in benznidazole sensitive parasites, relative to the control parasites transfected with GFP (pTREX-GFP), confirmed by quantitative RT-PCR and northern blot analysis. 15.6fold and 4.6fold overexpressions are detected for the two different techniques, respectively up

General Information

EC Number General Information Comment Organism
2.4.2.7 additional information Trypanosoma cruzi regulation is mainly posttranscriptional Trypanosoma cruzi
2.4.2.7 physiological function expression and role of adenine phosphoribosyltransferase (APRT) in Trypanosoma cruzi resistance to benznidazole (Bz), overview Trypanosoma cruzi