Any feedback?
Literature summary extracted from
- Production and characterization of a recombinant thermophilic trehalose synthase from Thermus antranikianii (2020), J. Biosci. Bioeng., 129, 418-422 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | synthesis | good application potential of the recombinant enzyme in the efficient conversion of trehalose from maltose | Thermus antranikianii |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.245 | recombinantly expressed in Escherichia coli BL21(DE3) | Thermus antranikianii |
| 5.4.99.16 | recombinantly expressed in Escherichia coli BL21(DE3) | Thermus antranikianii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.16 | Ca2+ | 10 mM, 29% loss of activity | Thermus antranikianii |  |
| 5.4.99.16 | Co2+ | 1 mM, 56% loss of activity | Thermus antranikianii | |
| 5.4.99.16 | Cu2+ | 1 mM, 83% loss of activity | Thermus antranikianii | |
| 5.4.99.16 | SDS | 1 mM, 99% loss of activity | Thermus antranikianii | |
| 5.4.99.16 | Tris | 1 mM, 46% loss of activity | Thermus antranikianii | |
| 5.4.99.16 | Zn2+ | 1 mM, 33% loss of activity | Thermus antranikianii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.16 | 73.2 | - |
alpha,alpha-trehalose | pH 7.0, 60°C | Thermus antranikianii | |
| 5.4.99.16 | 112.43 | - |
maltose | pH 7.0, 60°C | Thermus antranikianii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 112000 | - |
SDS-PAGE | Thermus antranikianii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.245 | Thermus antranikianii | WP_028494267 | - |
- |
| 5.4.99.16 | Thermus antranikianii | WP_028494267 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | - |
Thermus antranikianii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | alpha,alpha-trehalose | 1.48fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose | Thermus antranikianii | maltose | - |
r | |
| 5.4.99.16 | maltose | 1.48fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose | Thermus antranikianii | alpha,alpha-trehalose | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | ? | x * 112000, SDS-PAGE | Thermus antranikianii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.245 | TaTS | - |
Thermus antranikianii |
| 5.4.99.16 | TaTS | - |
Thermus antranikianii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.245 | 30 | - |
maximum trehalose yield (76.8%) | Thermus antranikianii |
| 2.4.1.245 | 60 | - |
highest activity (76.8%) | Thermus antranikianii |
| 5.4.99.16 | 60 | - |
highest activity (76.8%) | Thermus antranikianii |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 40 | 80 | 40°C: about 40% of maximal activity, 80°C: about 50% of maximal activity | Thermus antranikianii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 70 | - |
6 h, 5% loss of activity | Thermus antranikianii |
| 5.4.99.16 | 80 | - |
6 h, 50% loss of activity | Thermus antranikianii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.16 | 64.84 | - |
alpha,alpha-trehalose | pH 7.0, 60°C | Thermus antranikianii | |
| 5.4.99.16 | 148.04 | - |
maltose | pH 7.0, 60°C | Thermus antranikianii | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.245 | 7 | - |
highest activity and maximum trehalose yield | Thermus antranikianii |
| 5.4.99.16 | 7 | - |
highest activity and maximum trehalose yield | Thermus antranikianii |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 6 | 10 | stable for 6 h | Thermus antranikianii |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.16 | 0.885 | - |
alpha,alpha-trehalose | pH 7.0, 60°C | Thermus antranikianii | |
| 5.4.99.16 | 1.31 | - |
maltose | pH 7.0, 60°C | Thermus antranikianii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
