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Literature summary extracted from

  • Hiraga, K.; Kochi, H.; Motokawa, Y.; Kikuchi, G.
    Enzyme complex nature of the reversible glycine cleavage system of cock liver mitochondria (1972), J. Biochem., 72, 1285-1289 .
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.1.27 mitochondrion associated to inner membrane Gallus gallus 5739
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.27 40000
-
 T-protein, gel fuiltration Gallus gallus
1.4.1.27 220000
-
 complex of P-, L-protein, gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.27 Gallus gallus P15505 i.e. component P-protein, glycine dehydrogenase, cf. EC 1.4.4.2
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.27 isolation from mitochondria by ammunium sulfate fractionation and gel filtration Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.27 liver
-
 Gallus gallus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.27 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
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 Gallus gallus glycine + tetrahydrofolate + NAD+
-
 r
1.4.1.27 glycine + tetrahydrofolate + NAD+
-
 Gallus gallus 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.27 multimer x * 220000, complex of P-, L-protein, x * 40000, T-protein, gel filtration Gallus gallus

General Information

EC Number General Information Comment Organism
1.4.1.27 physiological function the reversible glycine cleavage system in liver mitochondria involves four enzyme proteins designated as P-protein (a pyridoxal phosphate requiring protein), H-protein (a hydrogen carrier protein), L-protein (exhibiting a lipoamide dehydrogenase activity) and T-protein (a H4-folate requiring protein). All three protein fractions obtained during purification are essential for the overall reactions of glycine cleavage and glycine synthesis, while only P-, L-protein and H-protein are required for the glycine-14CO2 exchange Gallus gallus