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Literature summary extracted from

  • Kulikova, V.V.; Revtovich, S.V.; Bazhulina, N.P.; Anufrieva, N.V.; Kotlov, M.I.; Koval, V.S.; Morozova, E.A.; Hayashi, H.; Belyi, Y.F.; Demidkina, T.V.
    Identification of O-acetylhomoserine sulfhydrylase, a putative enzyme responsible for methionine biosynthesis in Clostridioides difficile Gene cloning and biochemical characterizations (2019), IUBMB Life, 71, 1815-1823 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 L-methionine product inhibition Clostridioides difficile
2.5.1.49 L-propargylglycine irreversible, rapid inactivation; suicide inhibitor Clostridioides difficile
2.5.1.49 Ngamma-acetyl-L-2,4-diaminobutyric acid competitive; competitive and reversible inhibition Clostridioides difficile

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 0.4
-
 hydrogen sulfide pH 7.5, 25°C Clostridioides difficile
2.5.1.49 0.4
-
 methanethiol pH 7.5, 25°C Clostridioides difficile
2.5.1.49 0.6
-
 O-acetyl-L-homoserine pH 7.5, 25°C Clostridioides difficile

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.49 180000
-
 gel filtration Clostridioides difficile
2.5.1.49 180000
-
 gel fitration Clostridioides difficile

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Clostridioides difficile Q187D4
-
-
2.5.1.49 Clostridioides difficile 630 Q187D4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 50
-
 pH 7.5, 25°C Clostridioides difficile

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 O-acetyl-L-homoserine + hydrogen sulfide
-
 Clostridioides difficile L-homocysteine + acetate
-
 ?
2.5.1.49 O-acetyl-L-homoserine + hydrogen sulfide
-
 Clostridioides difficile 630 L-homocysteine + acetate
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methanethiol
-
 Clostridioides difficile L-methionine + acetate
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methanethiol
-
 Clostridioides difficile 630 L-methionine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.49 tetramer 4 * 46000, SDS-PAGE, 4 * 46355, calculated from sequence Clostridioides difficile

Synonyms

EC Number Synonyms Comment Organism
2.5.1.49 MetY
-
 Clostridioides difficile
2.5.1.49 OAHS
-
 Clostridioides difficile
2.5.1.49 Q187D4
-
 Clostridioides difficile

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.49 94.12
-
 O-acetyl-L-homoserine pH 7.5, 25°C Clostridioides difficile
2.5.1.49 96.65
-
 hydrogen sulfide pH 7.5, 25°C Clostridioides difficile
2.5.1.49 96.65
-
 methanethiol pH 7.5, 25°C Clostridioides difficile

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.49 7.5
-
 higher activity is shown in potassium phosphate buffer compared to sodium phosphate and Tris-HCl buffers Clostridioides difficile

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate
-
 Clostridioides difficile
2.5.1.49 pyridoxal 5'-phosphate the absorption spectrum of the holoenzyme at pH 7.5 shows a major band in the region of 415-416 nm corresponding to ketoenamine tautomer of the internal aldimines Clostridioides difficile

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.49 0.04
-
 Ngamma-acetyl-L-2,4-diaminobutyric acid pH 7.5, 25°C Clostridioides difficile
2.5.1.49 9
-
 L-methionine pH 7.5, 25°C Clostridioides difficile

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.49 160
-
 O-acetyl-L-homoserine pH 7.5, 25°C Clostridioides difficile
2.5.1.49 240
-
 hydrogen sulfide pH 7.5, 25°C Clostridioides difficile
2.5.1.49 240
-
 methanethiol pH 7.5, 25°C Clostridioides difficile