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Literature summary extracted from

  • Miyazawa, K.; Yoshimi, A.; Kasahara, S.; Sugahara, A.; Koizumi, A.; Yano, S.; Kimura, S.; Iwata, T.; Sano, M.; Abe, K.
    Molecular mass and localization of alpha-1,3-glucan in cell wall control the degree of hyphal aggregation in liquid culture of Aspergillus nidulans (2018), Front. Microbiol., 9, 2623 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.183 gene agsA, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans Aspergillus nidulans
2.4.1.183 gene agsB, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans Aspergillus nidulans
2.4.1.183 gene agsB, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.183 additional information overexpression of agsA in Aspergillus nidulans under the control of a constitutive promoter in the genetic background of agsB disruptants, the native promoter is replaced by the constitutive tef1 promoter. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan. The agsA gene is disrupted by using the Cre/loxP marker recycling system. Generation of an agsA deletion mutant strain agsADELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan Aspergillus nidulans
2.4.1.183 additional information overexpression of agsB in Aspergillus nidulans under control of a constitutive promoter in the genetic background of agsA disruptants, the native promoter is replaced by the constitutive tef1 promoter. Generation of an agsB deletion mutant strain agsBDELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan Aspergillus nidulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.183 cell wall
-
 Aspergillus nidulans 5618
-
2.4.1.183 cell wall
-
 Aspergillus oryzae 5618
-
2.4.1.183 intracellular
-
 Aspergillus nidulans 5622
-
2.4.1.183 additional information alpha-1,3-glucan is located in the inner layer in the agsA overexpressing strain Aspergillus nidulans
-
-
2.4.1.183 additional information alpha-1,3-glucan is located in the outer layer of the cell wall in the agsB overexpressing strain Aspergillus nidulans
-
-
2.4.1.183 additional information alpha-1,3-glucan is located in the outer layer of the cell wall in the agsB overexpressing strain Aspergillus oryzae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n Aspergillus nidulans
-
 UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n Aspergillus oryzae
-
 UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n Aspergillus nidulans ABPU1
-
 UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.183 Aspergillus nidulans
-
-
-
2.4.1.183 Aspergillus nidulans ABPU1
-
-
-
2.4.1.183 Aspergillus oryzae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.183 huelle cell nursing cells of cleistothecia Aspergillus nidulans
-
2.4.1.183 hypha
-
 Aspergillus nidulans
-
2.4.1.183 hypha
-
 Aspergillus oryzae
-
2.4.1.183 mycelium
-
 Aspergillus nidulans
-
2.4.1.183 mycelium
-
 Aspergillus oryzae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n
-
 Aspergillus nidulans UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n
-
 Aspergillus oryzae UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n
-
 Aspergillus nidulans ABPU1 UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.183 agsA
-
 Aspergillus nidulans
2.4.1.183 agsB
-
 Aspergillus nidulans
2.4.1.183 agsB
-
 Aspergillus oryzae
2.4.1.183 alpha-1,3-glucan synthase
-
 Aspergillus nidulans
2.4.1.183 alpha-1,3-glucan synthase
-
 Aspergillus oryzae

General Information

EC Number General Information Comment Organism
2.4.1.183 malfunction the disruption of the agsA gene does not alter or slightly increase the alpha-1,3-glucan content compared to wild-type. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan Aspergillus nidulans
2.4.1.183 malfunction the disruption of the agsB gene leads to loss of alpha-1,3-glucan. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan Aspergillus nidulans
2.4.1.183 malfunction the disruption of the agsB gene leads to the loss of alpha-1,3-glucan. Overexpression of agsB increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan. The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains Aspergillus oryzae
2.4.1.183 additional information determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the inner layer in the agsA overexpressing strain Aspergillus nidulans
2.4.1.183 additional information determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the outer layer of the cell wall in the agsB overexpressing strain Aspergillus nidulans
2.4.1.183 additional information determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the outer layer of the cell wall in the agsB overexpressing strain Aspergillus oryzae
2.4.1.183 physiological function alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular Aspergillus nidulans
2.4.1.183 physiological function alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions Aspergillus nidulans
2.4.1.183 physiological function alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions Aspergillus oryzae