Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mine, S.; Niiyama, M.; Hashimoto, W.; Ikegami, T.; Koma, D.; Ohmoto, T.; Fukuda, Y.; Inoue, T.; Abe, Y.; Ueda, T.; Morita, J.; Uegaki, K.; Nakamura, T.
    Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N-diacetylchitobiose deacetylase (2014), FEBS J., 281, 2584-2596 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.136 no expression in the soluble fraction of Escherichia coli harboring the expression plasmid. Insertion of the target genes into the chromosome of Escherichia coli yields the soluble recombinant protein Pyrococcus horikoshii
3.5.1.136 no expression in the soluble fraction of Escherichia coli harboring the expression plasmid. Insertion of the target genes into the chromosome of Escherichia coli yields the soluble recombinant protein Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.136 hanging-drop vapor-diffusion method, the crystal structure is determined at resolutions of 2.0 A Pyrococcus horikoshii
3.5.1.136 the crystal structure is determined at resolutions of 1.54 A Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.136 D46A complete loss of activity Pyrococcus horikoshii
3.5.1.136 H264A complete loss of activity Pyrococcus horikoshii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.136 Zn2+ the catalytic Zn2+ is situated at the end of the intermolecular cleft (which is formed by two polypeptides in the trimeric assembly) is coordinated by three side chain ligands from His44, Asp47, and His155, and by a phosphate ion derived from the crystallization reservoir solution Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.136 N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O Pyrococcus furiosus
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O Pyrococcus furiosus ATCC 43587
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii ATCC 700860
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.136 Pyrococcus furiosus Q8U3V1
-
-
3.5.1.136 Pyrococcus furiosus ATCC 43587 Q8U3V1
-
-
3.5.1.136 Pyrococcus horikoshii O58235
-
-
3.5.1.136 Pyrococcus horikoshii ATCC 700860 O58235
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.136
-
 Pyrococcus horikoshii
3.5.1.136
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.136 N,N'-diacetylchitobiose + H2O
-
 Pyrococcus horikoshii beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O
-
 Pyrococcus furiosus beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O
-
 Pyrococcus furiosus ATCC 43587 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O
-
 Pyrococcus horikoshii ATCC 700860 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.136 hexamer composed of two trimers Pyrococcus horikoshii
3.5.1.136 hexamer composed of two trimers Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.136 Dac
-
 Pyrococcus horikoshii
3.5.1.136 Dac
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.136 85
-
 thermostable up to Pyrococcus horikoshii
3.5.1.136 85
-
 thermostable up to Pyrococcus furiosus